PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1633 [new locus tag: NWMN_RS09175 ]
• pan locus tag^?: SAUPAN004407000
• symbol: murC
• pan gene symbol^?: murC
• synonym:
• product: UDP-N-acetylmuramate--L-alanine ligase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1633 [new locus tag: NWMN_RS09175 ]
• symbol: murC
• product: UDP-N-acetylmuramate--L-alanine ligase
• replicon: chromosome
• strand: -
• coordinates: 1818622..1819935
• length: 1314
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330958 NCBI
• RefSeq: YP_001332667 NCBI
• BioCyc:
• MicrobesOnline: 3707185 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGACACACTATCATTTTGTCGGAATTAAAGGTTCTGGCATGAGTTCATTAGCACAAATC
  ATGCATGATTTAGGACATGAAGTTCAAGGATCGGATATTGAGAACTACGTATTTACAGAA
  GTTGCTCTTAGAAATAAGGGGATAAAAATATTACCATTTGATGCTAATAACATAAAAGAA
  GATATGGTAGTTATACAAGGTAATGCATTCGCGAGTAGCCATGAAGAAATAGTACGTGCA
  CATCAATTGAAATTAGATGTTGTAAGTTATAATGATTTTTTAGGACAGATTATTGATCAA
  TATACTTCAGTAGCTGTAACTGGTGCACATGGTAAAACTTCTACAACAGGTTTATTATCA
  CATGTTATGAATGGTGATAAAAAGACTTCATTTTTAATTGGTGATGGCACAGGTATGGGA
  TTGCCTGAAAGTGATTATTTCGCTTTTGAGGCATGTGAATATAGACGTCACTTTTTAAGT
  TATAAACCTGATTACGCAATTATGACAAATATTGATTTCGATCATCCTGATTATTTTAAA
  GATATTAATGATGTTTTTGATGCATTCCAAGAAATGGCACATAATGTTAAAAAAGGTATT
  ATTGCTTGGGGTGATGATGAACATCTACGTAAAATTGAAGCAGATGTTCCAATTTATTAT
  TATGGATTTAAAGATTCGGATGACATTTATGCTCAAAATATTCAAATTACGGATAAAGGT
  ACTGCTTTTGATGTGTATGTGGATGGTGAGTTTTATGATCACTTCCTGTCTCCACAATAT
  GGTGACCATACAGTTTTAAATGCATTAGCTGTAATTGCGATTAGTTATTTAGAGAAGCTA
  GATGTTACAAATATTAAAGAAGCATTAGAAACGTTTGGTGGTGTTAAACGTCGTTTCAAT
  GAAACTACAATTGCAAATCAAGTTATTGTAGATGATTATGCACACCATCCAAGAGAAATT
  AGTGCTACAATTGAAACAGCACGAAAGAAATATCCACATAAAGAAGTTGTTGCAGTATTT
  CAACCACACACTTTCTCTAGAACACAGGCATTTTTAAATGAATTTGCAGAAAGTTTAAGT
  AAAGCAGATCGTGTATTCTTATGTGAAATTTTTGGATCAATTAGAGAAAATACTGGCGCA
  TTAACGATACAAGATTTAATTGATAAAATTGAAGGTGCATCGTTAATTAATGAAGATTCT
  ATTAATGTATTAGAACAATTTGATAATGCTGTTATTTTATTTATGGGTGCAGGTGATATT
  CAAAAATTACAAAATGCATATTTAGATAAATTAGGCATGAAAAATGCGTTTTAA
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⊟Protein

⊟General

• locus tag: NWMN_1633 [new locus tag: NWMN_RS09175 ]
• symbol: MurC
• description: UDP-N-acetylmuramate--L-alanine ligase
• length: 437
• theoretical pI: 4.77031
• theoretical MW: 49187.9
• GRAVY: -0.222197

⊟Function

• reaction:
  EC 6.3.2.8?  ExPASy
  UDP-N-acetylmuramate--L-alanine ligase ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 416.7)
  and 6 more
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (TIGR01081; HMM-score: 171.9)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 78.6)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 75.6)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 67.2)
  Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 25.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid bifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 21.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined Mur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 87.3)
  NADP_Rossmann (CL0063) Mur_ligase; Mur ligase family, catalytic domain (PF01225; HMM-score: 81.1)
  no clan defined Mur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 73.2)
  and 1 more
  SGNH_hydrolase (CL0264) DUF459; Protein of unknown function (DUF459) (PF04311; HMM-score: 11.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.018125
  • TAT(Tat/SPI): 0.001102
  • LIPO(Sec/SPII): 0.001738
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221845 NCBI
• RefSeq: YP_001332667 NCBI
• UniProt: A6QHS3 UniProt

⊟Protein sequence

• MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALRNKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYTSVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLSYKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPIYYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISYLEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHKEVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEGASLINEDSINVLEQFDNAVILFMGAGDIQKLQNAYLDKLGMKNAF

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1631 < NWMN_1632 < murC < NWMN_1634 < NWMN_1635 < NWMN_1636 < NWMN_1637 < NWMN_1638

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications