PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1400 [new locus tag: NWMN_RS07905 ]
• pan locus tag^?: SAUPAN004025000
• symbol: srrA
• pan gene symbol^?: srrA
• synonym:
• product: DNA-binding response regulator SrrA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1400 [new locus tag: NWMN_RS07905 ]
• symbol: srrA
• product: DNA-binding response regulator SrrA
• replicon: chromosome
• strand: -
• coordinates: 1570264..1570989
• length: 726
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332504 NCBI
• RefSeq: YP_001332434 NCBI
• BioCyc:
• MicrobesOnline: 3706952 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGTCGAACGAAATACTTATCGTAGATGATGAGGATAGAATCAGAAGATTACTTAAAATG
  TATTTAGAAAGAGAATCTTTTGAAATCCATGAAGCAAGTAATGGCCAAGAGGCTTATGAA
  CTTGCAATGGAGAATAATTATGCTTGCATACTACTAGATTTAATGTTGCCTGAAATGGAT
  GGTATCCAGGTGGCAACTAAATTGCGTGAACATAAACAAACACCGATTATTATGTTGACT
  GCTAAAGGTGAAGAAACAAACCGTGTTGAAGGTTTTGAATCTGGTGCAGATGATTATATC
  GTCAAACCATTTTCACCAAGAGAAGTAGTCTTAAGAGTTAAAGCACTTCTAAGAAGAACG
  CAATCTACAACTGTAGAACAAAGCGAACCTCACGCACGTGATGTGATTGAATTTAAACAT
  TTAGAAATAGATAATGATGCACATCGCGTACTTGCTGATAATCAAGAAGTTAATTTGACT
  CCTAAAGAGTACGAATTATTAATATATTTAGCTAAAACACCAAATAAAGTATTTGACCGT
  GAACAATTATTAAAAGAAGTTTGGCATTATGAATTCTATGGTGATTTAAGAACAGTTGAT
  ACTCATGTTAAACGACTTAGAGAAAAGTTAAATCGTGTGTCTAGTGAAGCTGCGCATATG
  ATTCAAACAGTCTGGGGCGTTGGGTATAAATTTGAGGTTAAATCTAATGATGAGCCGGCT
  AAATAG
  60
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  726
  

⊟Protein

⊟General

• locus tag: NWMN_1400 [new locus tag: NWMN_RS07905 ]
• symbol: SrrA
• description: DNA-binding response regulator SrrA
• length: 241
• theoretical pI: 5.02595
• theoretical MW: 28160.8
• GRAVY: -0.575519

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 245.3)
  Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 245.3)
  and 8 more
  Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 182.9)
  proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 115.6)
  Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 69.2)
  Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 59)
  Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 59)
  Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 59)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 34.9)
  Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 31.6)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 111.7)
  HTH (CL0123) Trans_reg_C; Transcriptional regulatory protein, C terminal (PF00486; HMM-score: 97.9)
  and 1 more
  GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 10
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001191
  • TAT(Tat/SPI): 0.000151
  • LIPO(Sec/SPII): 0.000213
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221612 NCBI
• RefSeq: YP_001332434 NCBI
• UniProt: A0A0H3KGL8 UniProt

⊟Protein sequence

• MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAMENNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVKPFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLTPKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAAHMIQTVWGVGYKFEVKSNDEPAK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: srrB < srrA

⊟Regulation

• regulator: Rex* (repression) regulon

  Rex*

  (TF)

  important in Energy metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications