PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0995 [new locus tag: NWMN_RS05590 ]
• pan locus tag^?: SAUPAN001425000
• symbol: NWMN_0995
• pan gene symbol^?: —
• synonym:
• product: phage anti-repressor protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0995 [new locus tag: NWMN_RS05590 ]
• symbol: NWMN_0995
• product: phage anti-repressor protein
• replicon: chromosome
• strand: +
• coordinates: 1102614..1103402
• length: 789
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330599 NCBI
• RefSeq: YP_001332029 NCBI
• BioCyc:
• MicrobesOnline: 3706546 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGCAAGCATTACAAACATTTAATTTTAAAGAGCTACCAGTAAGAACAGTGGAAATTGAA
  AACGAACCTTATTTTGTAGGAAAAGATATTGCTGAAATTTTAGGATATGCAAGGGCAGAC
  AATGCCATCAGAAATCATGTTGATAGCGAGGACAAGCTGACGCACCAATTTAGTGCATCA
  GGTCAAAACAGAAATATGATCATTATCAATGAATCAGGATTATACAGTTTAATCTTTGAC
  GCTTCTAAACAAAGTAAAAACGAAAAAATCAGAGAAACCGCTCGAAAATTCAAACGATGG
  GTAACATCAGACGTCCTACCAGCCATTCGCAAACACGGTATCTACGCAACAGACAATGTA
  ATTGAACAAACATTAAAAGATCCAGACTACATTATTACAGTGTTGACTGAGTATAAGAAA
  GAAAAAGAGCAAAACTTACTTTTACAACAAGAAATCGGAGAACTAAAACCCAAAGCAGAC
  TATGTAGATGAAATCTTAAAGTCAACTGGCACATTAGCCACAACTCAAATCGCGGCAGAC
  TACGGTATATCAGCACAAAAGTTAAACAAACTACTACACGAAGCTAGACTACAACGAAAA
  GTAAATAAACAGTGGGTGCTTTACTCAGAACACATGGGCAAGAGTTACACAGAATCAGAC
  ACTATACCAATTGTACGCTCTGACGGTAGAGAAGACACAGTTTTACAAACTAGATGGACA
  CAAAAAGGTAGATTGAAAATACATGAAATCATGACTGAATTCGGTTATGAAGCTAACGTA
  ACTGCTTAA
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⊟Protein

⊟General

• locus tag: NWMN_0995 [new locus tag: NWMN_RS05590 ]
• symbol: NWMN_0995
• description: phage anti-repressor protein
• length: 262
• theoretical pI: 7.18406
• theoretical MW: 30139.9
• GRAVY: -0.604962

⊟Function

• TIGRFAM:
• TheSEED: data available for COL, NCTC8325
• PFAM:
  no clan defined ANT; Phage antirepressor protein KilAC domain (PF03374; HMM-score: 101.8)
  Bro-N; BRO family, N-terminal domain (PF02498; HMM-score: 96.3)
  and 2 more
  P22_AR_N; P22_AR N-terminal domain (PF10547; HMM-score: 19.1)
  HTH (CL0123) HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006449
  • TAT(Tat/SPI): 0.000291
  • LIPO(Sec/SPII): 0.000415
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221207 NCBI
• RefSeq: YP_001332029 NCBI
• UniProt: A0A0H3KC58 UniProt

⊟Protein sequence

• MQALQTFNFKELPVRTVEIENEPYFVGKDIAEILGYARADNAIRNHVDSEDKLTHQFSASGQNRNMIIINESGLYSLIFDASKQSKNEKIRETARKFKRWVTSDVLPAIRKHGIYATDNVIEQTLKDPDYIITVLTEYKKEKEQNLLLQQEIGELKPKADYVDEILKSTGTLATTQIAADYGISAQKLNKLLHEARLQRKVNKQWVLYSEHMGKSYTESDTIPIVRSDGREDTVLQTRWTQKGRLKIHEIMTEFGYEANVTA

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications