PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0491 [new locus tag: NWMN_RS02870 ]
• pan locus tag^?: SAUPAN002297000
• symbol: cysE
• pan gene symbol^?: cysE
• synonym:
• product: serine acetyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0491 [new locus tag: NWMN_RS02870 ]
• symbol: cysE
• product: serine acetyltransferase
• replicon: chromosome
• strand: +
• coordinates: 566980..567627
• length: 648
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331965 NCBI
• RefSeq: YP_001331525 NCBI
• BioCyc:
• MicrobesOnline: 3706038 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATCTTGTTAAAAAGAATGAGAGACGATATAAAAATGGTATTTGAGCAGGATCCAGCG
  GCACGTTCAACATTAGAAGTCATTACAACGTATGCAGGTTTACATGCAGTTTGGAGTCAT
  TTGATTGCACATAAGTTATACAACCAAAAAAAATATGTTGCAGCACGCGCGATATCTCAA
  ATTTCAAGATTTTTCACAGGTATAGAAATCCATCCAGGTGCTAAAATTGGAAAGCGTCTA
  TTTATAGATCATGGTATGGGCGTTGTAATAGGAGAAACATGTACAATTGGTGATAATGTG
  ACAATCTATCAAGGCGTGACACTTGGTGGGACAGGGAAAGAAAGAGGGAAAAGACACCCA
  GATATAGGAGACAATGTTTTAATAGCAGCCGGTGCGAAAGTTTTAGGAAATATTAAAATA
  AATTCAAATGTAAATATTGGTGCAAATTCAGTTGTTTTACAATCAGTTCCAAGTTATTCA
  ACGGTTGTTGGTATACCAGGACATATTGTTAAGCAAGATGGTGTTCGAGTTGGAAAAACA
  TTTGATCATCGCCATCTACCTGATCCAATTTATGAACAAATTAAGCATTTAGAACGACAA
  CTTGAAAAGACTAGGAATGGAGAGATTCAAGATGATTACATTATATAA
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⊟Protein

⊟General

• locus tag: NWMN_0491 [new locus tag: NWMN_RS02870 ]
• symbol: CysE
• description: serine acetyltransferase
• length: 215
• theoretical pI: 9.85567
• theoretical MW: 23754.2
• GRAVY: -0.156279

⊟Function

• reaction:
  EC 2.3.1.30?  ExPASy
  Serine O-acetyltransferase Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine
• TIGRFAM:
  Metabolism Amino acid biosynthesis Serine family serine O-acetyltransferase (TIGR01172; EC 2.3.1.30; HMM-score: 250.8)
  and 13 more
  colanic acid biosynthesis acetyltransferase WcaB (TIGR04016; EC 2.3.1.-; HMM-score: 73.2)
  sugar O-acyltransferase, sialic acid O-acetyltransferase NeuD family (TIGR03570; HMM-score: 56.8)
  2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (TIGR03532; EC 2.3.1.89; HMM-score: 52.2)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase LpxD (TIGR01853; EC 2.3.1.191; HMM-score: 39.8)
  phosphonate metabolim protein, transferase hexapeptide repeat family (TIGR03308; HMM-score: 31.7)
  non-ribosomal peptide synthetase terminal domain of unknown function (TIGR02353; HMM-score: 20.6)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase (TIGR01852; EC 2.3.1.129; HMM-score: 20.1)
  colanic acid biosynthesis acetyltransferase WcaF (TIGR04008; EC 2.3.1.-; HMM-score: 17.7)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 17.5)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 17.5)
  Metabolism Central intermediary metabolism Amino sugars UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 17.5)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR03991; EC 2.3.1.157,2.7.7.23; HMM-score: 9.2)
  Metabolism Central intermediary metabolism Amino sugars UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR03991; EC 2.3.1.157,2.7.7.23; HMM-score: 9.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  HEXAPEP (CL0536) Hexapep; Bacterial transferase hexapeptide (six repeats) (PF00132; HMM-score: 57)
  and 2 more
  Hexapep_2; Hexapeptide repeat of succinyl-transferase (PF14602; HMM-score: 19.4)
  no clan defined SATase_N; Serine acetyltransferase, N-terminal (PF06426; HMM-score: 18.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 10
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003478
  • TAT(Tat/SPI): 0.000756
  • LIPO(Sec/SPII): 0.001007
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220703 NCBI
• RefSeq: YP_001331525 NCBI
• UniProt: A0A0H3K767 UniProt

⊟Protein sequence

• MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAHKLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTIYQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYSTVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: cysE > cysS > NWMN_0493 > NWMN_0494 > NWMN_0495 > NWMN_0496

⊟Regulation

• regulator: T-box(Cys) (transcription antitermination) regulon

  T-box(Cys)

  (RNA)

  important in Amino acid metabolism; regulatory site identified based on RegPrecise data for N315 RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications