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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0247 [new locus tag: NWMN_RS01365 ]
  • pan locus tag?: SAUPAN001223000
  • symbol: NWMN_0247
  • pan gene symbol?: —
  • synonym:
  • product: formate/nitrite transporter family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0247 [new locus tag: NWMN_RS01365 ]
  • symbol: NWMN_0247
  • product: formate/nitrite transporter family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 300845..301690
  • length: 846
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAAAAGTGAGGATGTCATTGTGAAGGAGAAACATATTAAATGGGATAAGATTTTTTAT
    GGGGACGACTGGGTAAATAACGTAGTCGAAACGATAAGAACAAAAGATATATTACAAAGC
    GTTTATTTAAAACGTTATTTATTACGCGCGATGATGGCAGGATTTATTATCGGGATTATT
    ACGGTCTTCGTATTATCAGTTAAAGCAACACACGAACCAGATTTACCGCCAGGCATTGTG
    AATATGGCCAGTGCCATTACATTCAGCTTTGCGTTAGTACTCATTTTATTTACAAACTCC
    GAACTACTAACCAGTAACTTCATGTACTTTACTGTAGGCCTGTATTATAAAGTAATTAAA
    CCAACTAGAGTATTGAAAATATTTTTATTATGCTTTGCAGGAAATATTTTAGGTGCTGCT
    ATTTTATTTAGTTTCATGCGTTTTTCAAATGTAATGACGCCAGATATGTTAAATCAGTTA
    TCAGCAGTTATAGAGCATAAAACGTTGTCTACTGGTTTTGTAAGTATATTAATGAAAGCG
    ATCTTCGCCAATTTCTTTATCAATATTTCATTAGTTATTGCCATGCAAATTGACGATGTA
    CTCGCAAAAATGTTTGTCATGATGTTCGGTGTTACCATATTCGCCTTTATGGGTTATGAA
    CACGTCGTTTACAACAGTTGCTTATTTATGGGTGGTTTAATTTATCAAGTTGATACATTG
    CACTTCATACCAGCTATTTCGAATATCGCTGCGGCTTTTATCGGTAACTATATTGGAGGC
    GGACTCATTATAGGTTTATTCTATGCTTACTTGAATGATCATCATCAATTTTATAAAAAT
    AATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    846

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0247 [new locus tag: NWMN_RS01365 ]
  • symbol: NWMN_0247
  • description: formate/nitrite transporter family protein
  • length: 281
  • theoretical pI: 8.58218
  • theoretical MW: 31789.6
  • GRAVY: 0.713523

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Anions formate/nitrite transporter (TIGR00790; HMM-score: 128.8)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids formate transporter FocA (TIGR04060; HMM-score: 107)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined Form_Nir_trans; Formate/nitrite transporter (PF01226; HMM-score: 165.2)
    and 2 more
    DUF1980; Domain of unknown function (DUF1980) (PF09323; HMM-score: 14.8)
    DUF2613; Protein of unknown function (DUF2613) (PF11021; HMM-score: 14.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 0
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008879
    • TAT(Tat/SPI): 0.000303
    • LIPO(Sec/SPII): 0.001984
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKSEDVIVKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAMMAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMYFTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHKTLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYNSCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Rex* (repression) regulon
    Rex*(TF)important in Energy metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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