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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002638
  • pan locus tag?: SAUPAN006381000
  • symbol: gtfA
  • pan gene symbol?: gtfA
  • synonym:
  • product: accessory Sec system glycosyltransferase GtfA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002638
  • symbol: gtfA
  • product: accessory Sec system glycosyltransferase GtfA
  • replicon: chromosome
  • strand: -
  • coordinates: 2650343..2651851
  • length: 1509
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGACAATATATAATATAAACTTTGGTATAGGCTGGGCGAGTAGCGGTGTTGAATATGCA
    CAAGCTTACCGTGCTAAATTATTGAGACGTACAAATCAAGACACTAAATTTGTGTTTTTA
    GATTTTATACAGTCAGAGAACATACAGACATTAACGCAAAACATTGGGTTTAAAGATCAA
    GAAGTTATTTGGTTGTATCAGTATTTTAGTGATATTTCTATAGCACCAACAACGTACACG
    TTAGATGACATTGAACGTGAGCTTGGTCATGAAATTAGTTTTAGAGAAAGAAATGGTAAG
    ATAGTCCGTCTATATTTTAATGGGAAATCATCATTTGTAACATGTTATCTACAAAATGAA
    CAAAAAGATGTTGTGGATCGTGCAGAATTTGTAATTAATAGTATGTTAGTACGTAAAGAT
    TTTTATAGTTATACGCGTATATTTTCTGAGTATTATGCACCAGCTGATAATAAAGCGAAA
    TTATATATGCGACAATTCTATAATGAAGATGGTTCCATCGCTTATAACGAATATATTGAT
    GGAGACAGCTCTATATATGTGTTTGAAGATGCGGTGTTGTATAACAAAACCGAATTTATT
    GCATACTTTTTACAACGATTGAATTTAACCCGAGACGATATTGTCATATTAGACCGCGCT
    TCTGATATTGGTCAGGCTGTTTTGCAGCATAAAGGTGATAGTAAAGTTGGTGTTGTGATT
    CATGCTGATCATTATAGTAATAATATGATGAGTGAGCAGCATATACTATGGAACAATTAT
    TATGAATATCAATTTTCAAAAGCTAAATACATTGATTTCTTTATTACAGCAACAGATATT
    CAAAATCATATGGTTTGTCGACAATTTGAGCAATATCAAGGTTATCGCCCACGTGTTTAT
    ACTATTCCTGTGGGAAGCATTGATGCATTATCATATCCAACATTATCAAGAAAGCCATAT
    GCGATGATTAGTGCATCTCGTTTAGCGAATGAGAAACATATTGATTGGCTTGTCAAAGCG
    GTTATCGTGGCTAAACGACAAGTGCCAGAATTAACATTTGATATTTATGGAGAAGGTAGC
    GAGAAGACGCGACTGCGTAAAATTATTGATACACACCGCGCACAAGATTATATTCGTTTA
    CTAGGGCATGTTAAACTTGATGAAATTTATAATGATTATGAGTTATTTCTTTCTGCATCA
    ACTAGCGAAGGTTTTGGGTTAACACTAATGGAAGCGGTAGGCTCAGGACTAGGTATGATT
    GGATTAAATGTGAATTATGGTAACCCAACCTTTATTCGAGATGGGGGAAATGGCTACTTG
    GTACCTTTTGATACCGACGAAGATAGTGTGGATGATGTTATCGCAAAACTAGCTCATGCC
    ATTGTAATGTATTTTAACAATGGTCCCCAGACACCACATGACATCTCTTATGAAGTGGCA
    CAGCAATTTATGACTCAAGATATTATTTTAAAATGGGAAACATTAGTTCAAGAGGTGCTA
    CATGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1509

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002638
  • symbol: GtfA
  • description: accessory Sec system glycosyltransferase GtfA
  • length: 502
  • theoretical pI: 4.91105
  • theoretical MW: 58201.2
  • GRAVY: -0.304781

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair accessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 870.5)
    and 9 more
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 53.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 49.1)
    colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 35.6)
    glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 20.1)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 19.5)
    sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 17.2)
    D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 16.3)
    PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 15.4)
    Unknown function Enzymes of unknown specificity glycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 15.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined GtfA_EBD; GtfA extended beta-sheet domain (PF22145; HMM-score: 146.9)
    and 4 more
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 84.8)
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 77.8)
    no clan defined DUF5503; Family of unknown function (DUF5503) (PF17607; HMM-score: 15.1)
    GT-B (CL0113) GT4-conflict; Family 4 Glycosyltransferase in conflict systems (PF20706; HMM-score: 14)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9527
    • Cytoplasmic Membrane Score: 0.0241
    • Cell wall & surface Score: 0.0005
    • Extracellular Score: 0.0227
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014868
    • TAT(Tat/SPI): 0.000543
    • LIPO(Sec/SPII): 0.002457
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQSENIQTLTQNIGFKDQEVIWLYQYFSDISIAPTTYTLDDIERELGHEISFRERNGKIVRLYFNGKSSFVTCYLQNEQKDVVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAKLYMRQFYNEDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILDRASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFITATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHIDWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYNDYELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGGNGYLVPFDTDEDSVDDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation; transcription unit predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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