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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002483
  • pan locus tag?: SAUPAN006131000
  • symbol: gntK
  • pan gene symbol?: gntK
  • synonym:
  • product: gluconokinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002483
  • symbol: gntK
  • product: gluconokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2488512..2490065
  • length: 1554
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAATATATGATTGGTGTCGACATTGGAACGACGAGTACCAAGTCAGTCTTATATGAT
    GAAAATGGAACATTCATCATGAAACATCAAATCGGCTATGATTTACACACACCAAACGTT
    GATGTCTCAGAAGAAAACCCAGATGAATTATTTGATGCGGTATTAATGACTATTAAATAT
    GTTATGAGAGAATCCAAAGTTAATAAAGATGATATTAAATTTGTGTCATTTAGTGCGCAA
    ATGCATAGCTTGATTGCGATGGATCAGAAACATCAAAGATTAACAAATAATATTACTTGG
    GCAGATAACCGCGCTGCAAAATATGCAACAGTAATAAATGAAGTGCATGATGGCAATGCG
    ATTTATCAGCGAACAGGTACGCCTATTCATCCTATGTCGCCTTTAGTGAAAATTTTTTGG
    ATGAAACATGAATGGCAAGATGTATTTCAACGTACTGCTAAGTTTGCAGATATTAAAACA
    TACATTTTCTATCATTTATTTGATACATATATCATTGATTATTCAATGGCTTCCGCAACA
    GGGATGTTTAATTTAGAAACATTAGATTGGGATGTTGGGGCATTAGAATTGCTTGGTATT
    TCCAAGGAAATGTTGCCGGAATTAGTGCCAACAACGTACGTAATGAAAGGCATGAAAGAA
    CGTTATGCAACATTAATGGGGCTTAATGAAGATACACCGTTTGTTATTGGTGCGAGTGAT
    GGGGTCCTTTCTAATTTGGGTGTCAATAGCGTTGGTAAAGGAGAAGTTGCTGTCACAATC
    GGTACATCTGGGGCGATTCGTACTGTGATAGATAAACCGCGTACTGATTACAAAGGTAGA
    ATATTTTGTTATGTCTTAACAGAGGACCACTACGTCATCGGAGGTCCTGTAAATAATGGT
    GGTGTCGTATTGAGATGGTTGCGCGACGAGTTGCTAGCGAGTGAAGTCGAAACTGCGAAA
    CGTCTCGGTGTTGATCCTTATGATGTCTTAACTCAAATTGCAAAGCGTGTTAAACCAGGT
    GCAGATGGTTTAATATTCCATCCCTATTTAGCTGGAGAACGTGCGCCGCTTTGGAACGCA
    AATGCAAGAGGTTCATTCTTCGGTTTAACTTTATCTCATAAAAAAGAACATATGATTCGC
    GCTGCATTAGAAGGTGTTCTTTACAATTTGTATACCGTCTACCTTGCACTCATTGAAGTA
    ATGAATGAAACGCCTAAGATGATAAAAGCAACAGGTGGTTTTGCGAAAAGTGAAGTATGG
    CGTCAAATGATGTCAGATATATTTGACACAGAGTTAGTGGTTCCTGAAAGCTATGAAAGT
    TCATGCTTAGGTGCCTGCGTGCTTGGACTTAAAGCTGTAGGTGACATTGAAGATTTTTCA
    ATCGTTTCATCGATGGTCGGTGCTACAAATAATCATATGCCGTTTGAAGAAAATGTCGCT
    GTTTACCAAGAGCTCGTATCCATTTTTATCAATTTAAGTCGTTCTTTAACAGAGAATTAT
    GAACAAATTGCAGATTTTCAACGCCAACATATGGCTGAAAATAAAACACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1554

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002483
  • symbol: GntK
  • description: gluconokinase
  • length: 517
  • theoretical pI: 5.23399
  • theoretical MW: 58103.2
  • GRAVY: -0.110058

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.12?  ExPASy
    Gluconokinase ATP + D-gluconate = ADP + 6-phospho-D-gluconate
  • TIGRFAM:
    Metabolism Energy metabolism Sugars gluconate kinase (TIGR01314; EC 2.7.1.12; HMM-score: 1005.4)
    and 8 more
    Metabolism Energy metabolism Sugars xylulokinase (TIGR01312; EC 2.7.1.17; HMM-score: 333.4)
    Metabolism Energy metabolism Other glycerol kinase (TIGR01311; EC 2.7.1.30; HMM-score: 209.2)
    Metabolism Energy metabolism Sugars L-fuculokinase (TIGR02628; EC 2.7.1.51; HMM-score: 115.5)
    FGGY-family pentulose kinase (TIGR01315; EC 2.7.1.-; HMM-score: 97.8)
    Metabolism Energy metabolism Sugars ribulokinase (TIGR01234; EC 2.7.1.16; HMM-score: 59.4)
    rhamnulokinase (TIGR02627; EC 2.7.1.5; HMM-score: 58.4)
    putative CoA-substrate-specific enzyme activase (TIGR00241; HMM-score: 20.8)
    benzoyl-CoA reductase, subunit A (TIGR02259; EC 1.3.7.8; HMM-score: 11.2)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Actin_ATPase (CL0108) FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 243.5)
    and 5 more
    FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 108.8)
    BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 31)
    Hydant_A_N; Hydantoinase/oxoprolinase N-terminal region (PF05378; HMM-score: 14.5)
    no clan defined DDE_Tnp_IS66_C; IS66 C-terminal element (PF13817; HMM-score: 13.3)
    HTH (CL0123) PSV_trans_reg_dom; PSV, transcriptional regulator domain (PF22376; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9711
    • Cytoplasmic Membrane Score: 0.0186
    • Cell wall & surface Score: 0.0024
    • Extracellular Score: 0.0079
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003874
    • TAT(Tat/SPI): 0.000098
    • LIPO(Sec/SPII): 0.000736
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSEENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQKHQRLTNNITWADNRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLVKIFWMKHEWQDVFQRTAKFADIKTYIFYHLFDTYIIDYSMASATGMFNLETLDWDVGALELLGISKEMLPELVPTTYVMKGMKERYATLMGLNEDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTDYKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIAKRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYTVYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGLKAVGDIEDFSIVSSMVGATNNHMPFEENVAVYQELVSIFINLSRSLTENYEQIADFQRQHMAENKTQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: GntR* (repression) regulon, CcpA regulon
    GntR*(TF)important in Gluconate utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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