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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002484
  • pan locus tag?: SAUPAN006132000
  • symbol: JSNZ_002484
  • pan gene symbol?: gntR
  • synonym:
  • product: GntR family transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002484
  • symbol: JSNZ_002484
  • product: GntR family transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 2490089..2490769
  • length: 681
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGATGTATGGATATCCAGAGAAATGGTTGGAAGGTATGACAACTGGAGAAGGTATCGCG
    GCAGAATTACGCTTAGGTATTGTGAATGGTCACATAGCTGAAGGTACGTTACTCACTGAA
    AATCAAATGGCAAAGCAATTTAATGTGAGTCGTTCGCCAATTCGAGATGCATTTAAATTA
    TTGCAACAAAATCAACTCATCCAATTAGAAAGAATGGGTGCACATGTGTTGCCGTTTGGG
    GAACAAGAAAAGAAAGAAATGTACGATTTGCGACTGATGCTAGAGTCTTTTGCATTTTCA
    AGAGTTAAAAATCAAGAGCGACTACCTATCGTAAAAGAAATGAAGAAACAACTTGAAATG
    ATGAAAGTGGCAGTAAAATTTGAGGATGCAGAATCATTTACGAAGCATGACTTTGAATTT
    CATGAAACATTAATCAAAGCATCTAATCATCAATATTTAAACTCATTTTGGAGTCATTTA
    AAACCAGTAATGATGGCACTCGTTTTAACATCCATGCGACAACGTATGCAACAGAACCCG
    CAAGATTTTGAACGCATACATCATAATCATCAAGTGTTTATTGATGCAGTGGAACAATAC
    GACAGTCAAATTTTGAAAGAAGCGTTTCACTTAAATTTCGACGATGTTGGTAAAGATATT
    GAAGGATTTTGGTTGAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    681

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002484
  • symbol: JSNZ_002484
  • description: GntR family transcriptional regulator
  • length: 226
  • theoretical pI: 6.35132
  • theoretical MW: 26595.4
  • GRAVY: -0.469912

⊟Function[edit | edit source]

  • TIGRFAM:
    phosphonate utilization associated transcriptional regulator (TIGR03338; HMM-score: 85.1)
    and 8 more
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02404; HMM-score: 26.5)
    Metabolism Transport and binding proteins Anions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 24.4)
    Signal transduction Regulatory functions DNA interactions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 24.4)
    Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 16.2)
    Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 16.2)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 12.9)
    Metabolism Fatty acid and phospholipid metabolism Degradation fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 12.9)
    Signal transduction Regulatory functions DNA interactions fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 12.9)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    FadR-C (CL0388) FCD; FCD domain (PF07729; HMM-score: 80.7)
    and 6 more
    HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 47.3)
    HTH_41; Helix-turn-helix domain (PF14502; HMM-score: 16.7)
    FadR-C (CL0388) FadR_C; FadR C-terminal domain (PF07840; HMM-score: 16.5)
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 14.1)
    ATP-grasp (CL0179) DUF1297; Domain of unknown function (DUF1297) (PF06973; HMM-score: 12.5)
    TIM_barrel (CL0036) DUF4996; Domain of unknown function (PF16387; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effector: Gluconate
  • genes regulated by GntR*, TF important in Gluconate utilizationsee RegPrecise for N315
    repression
    gntP* < gntK < gntR*

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9987
    • Cytoplasmic Membrane Score: 0.001
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0004
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003314
    • TAT(Tat/SPI): 0.000539
    • LIPO(Sec/SPII): 0.000578
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMAKQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRVKNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHLKPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGKDIEGFWLN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: GntR* (repression) regulon, CcpA regulon
    GntR*(TF)important in Gluconate utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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