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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002356
  • pan locus tag?: SAUPAN005915000
  • symbol: JSNZ_002356
  • pan gene symbol?: narK
  • synonym:
  • product: nitrate/nitrite transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002356
  • symbol: JSNZ_002356
  • product: nitrate/nitrite transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2354546..2355715
  • length: 1170
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTACAAAACAAAAGGTGGCTTTCAACTTACATTACAGACATTAAGTTTAGTGGTTGGG
    TTTATGGCTTGGAGTATAATTGCGCCATTAATGCCCTTTATTAAACAAGATGTCAATGTT
    ACTGAAGGTCAAATATCAATCATTTTAGCGATACCAGTTATTTTGGGATCGGTGCTCCGT
    GTGCCATTTGGTTATCTAACAAACATTGTTGGCGCTAAATGGGTATTCTTTACTAGTTTT
    ATCGTATTGTTATTCCCGATATTTTTCTTAAGCCAAGCACAAACACCGGGTATGTTAATG
    GCGTCAGGATTTTTCCTTGGTGTAGGTGGTGCAATTTTCTCAGTTGGTGTTACATCAGTT
    CCTAAATATTTCCCTAAAGAAAAAGTAGGTCTAGCAAATGGTATTTATGGTATGGGAAAT
    ATCGGTACAGCAGTTTCTTCATTTTTAGCACCGCCAATTGCGGGTATTATTGGTTGGCAA
    ACAACTGTTAGAAGTTACTTAATTATTATCGCTTTATTTGCATTAATTATGTTTATATTT
    GGTGATACACAAGAACGTAAAATTAAAGTGCCATTAATGGCACAAATGAAAACATTATCT
    AAAAACTACAAATTATATTACTTAAGTTATTGGTATTTCATTACTTTTGGTGCTTTTGTA
    GCATTTGGTATTTTCTTACCTAACTACTTAGTAAATCATTTTGGAATTGACAAAGTAGAT
    GCTGGTATTCGATCAGGTGTATTCATTGCGCTGGCAACATTCTTAAGACCAATAGGTGGC
    ATTTTAGGTGATAAATTTAATGCAGTTAAAGTATTGATGATTGATTTTGTTGTTATGATT
    ATCGGTGCCATTATTTTAGGTATTTCAGACCATATCGCATTATTCACTGTAGGCTGTTTA
    ACAATAAGTATTTGTGCAGGTATTGGTAACGGCTTAATCTTCAAATTAGTACCATCCTAC
    TTCTTAAATGAAGCGGGATCCGCAAATGGTATCGTATCAATGATGGGTGGTTTAGGAGGA
    TTCTTCCCACCACTAGTAATCACGTACGTAGCTAATTTAACAGGATCAAGTCATTTAGCA
    TTTATTTTCTTAGCGGTATTTGGATGTATTGCATTATTTACCATGCGTCATTTATACCAA
    AAAGAATATGGCTCATTGAAAAACGGTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1170

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002356
  • symbol: JSNZ_002356
  • description: nitrate/nitrite transporter
  • length: 389
  • theoretical pI: 9.92595
  • theoretical MW: 42148.2
  • GRAVY: 0.921851

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 197.7)
    and 9 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 58.2)
    Metabolism Transport and binding proteins Other cyanate transporter (TIGR00896; HMM-score: 58)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 50.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 44.7)
    multidrug resistance protein (TIGR00880; HMM-score: 36.3)
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 22.1)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 22.1)
    oxalate/formate antiporter (TIGR04259; HMM-score: 21.7)
    sodium-independent organic anion transporter (TIGR00805; HMM-score: 15.3)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 201.4)
    and 3 more
    OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 18.5)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 12.1)
    MFS (CL0015) MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 6.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9984
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0016
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.070673
    • TAT(Tat/SPI): 0.001376
    • LIPO(Sec/SPII): 0.020064
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMASGFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQTTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGAFVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDFVVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSMMGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, NreC (activation) regulon
    Rex*(TF)important in Energy metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]
    NreC(TF)important in Nitrate and nitrite respiration;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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