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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2333 [new locus tag: SAUSA300_RS12890 ]
  • pan locus tag?: SAUPAN005915000
  • symbol: narK
  • pan gene symbol?: narK
  • synonym:
  • product: nitrite extrusion protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2333 [new locus tag: SAUSA300_RS12890 ]
  • symbol: narK
  • product: nitrite extrusion protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2508369..2509538
  • length: 1170
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTACAAAACAAAAGGTGGCTTTCAACTTACATTACAAACATTAAGTTTAGTGGTTGGG
    TTTATGGCTTGGAGTATAATTGCGCCATTAATGCCCTTTATTAAACAAGATGTCAATGTT
    ACTGAAGGTCAAATATCAATCATTTTAGCGATACCAGTTATTTTGGGATCGGTGCTCCGT
    GTGCCATTTGGTTATTTAACAAACATTGTTGGCGCTAAATGGGTATTCTTTACTAGTTTT
    ATCGTATTGTTATTCCCGATATTTTTCTTAAGCCAAGCACAAACACCGGGTATGTTAATG
    GCTTCAGGATTTTTCCTTGGTGTAGGTGGTGCAATTTTCTCAGTTGGTGTTACATCAGTT
    CCTAAATATTTCCCTAAAGAAAAAGTAGGTCTAGCAAATGGTATTTATGGTATGGGAAAT
    ATCGGTACAGCAGTTTCTTCATTTTTAGCACCACCGATAGCGGGTATTATTGGTTGGCAA
    ACAACAGTTAGAAGTTACTTAATTATTATCGCTTTATTTGCATTAATTATGTTCATTTTT
    GGTGACACACAAGAACGTAAAATTAAAGTACCATTAATGGCTCAAATGAAAACATTATCT
    AAAAACTACAAATTATATTACTTAAGTTATTGGTATTTTATTACTTTTGGTGCTTTTGTA
    GCATTTGGTATTTTCTTACCTAACTACTTAGTAAATCATTTTGGAATTGACAAAGTAGAT
    GCTGGTATTCGATCAGGTGTATTCATTGCGCTGGCAACATTCTTAAGACCAATAGGTGGC
    ATTTTAGGTGATAAATTTAATGCAGTTAAAGTATTGATGATTGATTTTGTTGTTATGATT
    ATCGGTGCCATTATTTTAGGTATTTCAGACCATATCGCATTATTCACTGTAGGCTGTTTA
    ACAATAAGTATTTGTGCAGGTATTGGTAACGGCTTAATCTTCAAATTAGTACCATCATAC
    TTCTTAAATGAAGCGGGATCCGCAAATGGTATCGTATCAATGATGGGTGGTTTAGGAGGA
    TTCTTCCCACCACTAGTAATCACGTACGTAGCTAATTTAACAGGATCAAGTCATTTAGCA
    TTTATTTTCTTAGCGGTATTTGGATGTATTGCATTATTTACCATGCGTCATTTATACCAA
    AAAGAATATGGCTCATTGAAAAACGGTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1170

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2333 [new locus tag: SAUSA300_RS12890 ]
  • symbol: NarK
  • description: nitrite extrusion protein
  • length: 389
  • theoretical pI: 9.92595
  • theoretical MW: 42148.2
  • GRAVY: 0.921851

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 197.7)
    and 9 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 58.2)
    Metabolism Transport and binding proteins Other cyanate transporter (TIGR00896; HMM-score: 58)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 50.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 44.7)
    multidrug resistance protein (TIGR00880; HMM-score: 36.3)
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 22.1)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 22.1)
    oxalate/formate antiporter (TIGR04259; HMM-score: 21.7)
    sodium-independent organic anion transporter (TIGR00805; HMM-score: 15.3)
  • TheSEED  :
    • Nitrate/nitrite transporter
    Nitrogen Metabolism Nitrogen Metabolism - no subcategory Nitrate and nitrite ammonification  Nitrate/nitrite transporter
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 150.3)
    and 3 more
    OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 19)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 11.5)
    MFS (CL0015) MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 8.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.070673
    • TAT(Tat/SPI): 0.001376
    • LIPO(Sec/SPII): 0.020064
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMASGFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQTTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGAFVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDFVVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSMMGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: NreC* (activation) regulon, Rex (repression) regulon
    NreC*(TF)important in Nitrate and nitrite respiration; RegPrecise 
    Rex(TF)important in Energy metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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