PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_002350
• pan locus tag^?: SAUPAN005907000
• symbol: JSNZ_002350
• pan gene symbol^?: —
• synonym:
• product: pyridoxamine 5'-phosphate oxidase family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_002350
• symbol: JSNZ_002350
• product: pyridoxamine 5'-phosphate oxidase family protein
• replicon: chromosome
• strand: +
• coordinates: 2349874..2350296
• length: 423
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  
  ATGAGTAACTCACAAGCAATTCAAGCAATTGAAAACGTGTTAGCAACGTCAAAAGTTGGT
  GTATTATCAACTGCATATAATAACAAACCTAATAGTAGATATATGGTCTTTTATAATGAT
  GGTCTTACTTTATATACTAAAACGAATATCCATTCTGCTAAGGTCAAAGAAATTAAAGAA
  AACCCAGCAGCCTATGTATTGTTAGGTTACAATGATACGACAAATCGCAGTTTCGTTGAA
  ATGGAAGCCACGATAGAAGTAGTTACTGATCAAAAAGTAATCGATTGGTTATGGGAGACT
  CAAGATAAAAGCTTTTTCAGTTCAAAAGAAGACCCAGAACTTTGTGTTTTAAGAGTAGTT
  CCGCAATCCATTAAGCTAATGAATGATAAATCATTAGATACACCTATCAAAATCGATTTA
  TAA
  60
  120
  180
  240
  300
  360
  420
  423
  

⊟Protein

⊟General

• locus tag: JSNZ_002350
• symbol: JSNZ_002350
• description: pyridoxamine 5'-phosphate oxidase family protein
• length: 140
• theoretical pI: 4.9169
• theoretical MW: 15871
• GRAVY: -0.301429

⊟Function

• TIGRFAM:
  Unknown function Enzymes of unknown specificity PPOX class probable F420-dependent enzyme (TIGR03618; HMM-score: 28.1)
  and 4 more
  Unknown function Enzymes of unknown specificity PPOX class probable F420-dependent enzyme, Rv3369 family (TIGR03667; EC 1.-.-.-; HMM-score: 19.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxamine 5'-phosphate oxidase (TIGR00558; EC 1.4.3.5; HMM-score: 18.7)
  Unknown function Enzymes of unknown specificity PPOX class probable FMN-dependent enzyme, alr4036 family (TIGR04026; EC 1.-.-.-; HMM-score: 14.8)
  Unknown function Enzymes of unknown specificity PPOX class probable F420-dependent enzyme, Rv2061 family (TIGR03666; EC 1.-.-.-; HMM-score: 11.8)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  FMN-binding (CL0336) PNPOx_N; Pyridoxamine 5'-phosphate oxidase (PF01243; HMM-score: 59.9)
  Pyrid_ox_like; Pyridoxamine 5'-phosphate oxidase like (PF16242; HMM-score: 48.2)
  and 2 more
  Pyridox_ox_2; Pyridoxamine 5'-phosphate oxidase (PF12900; HMM-score: 18.7)
  SH3 (CL0010) Tudor_5; Histone methyltransferase Tudor domain 1 (PF18359; HMM-score: 14.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9845
  • Cytoplasmic Membrane Score: 0.0013
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0142
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.021369
  • TAT(Tat/SPI): 0.002074
  • LIPO(Sec/SPII): 0.015925
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTLYTKTNIHSAKVKEIKENPAAYVLLGYNDTTNRSFVEMEATIEVVTDQKVIDWLWETQDKSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SigB (activation) regulon

  SigB

  (sigma factor)

  controlling a large regulon involved in stress/starvation response and adaptation; transcription unit predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)   other strains

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications