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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001996
  • pan locus tag?: SAUPAN005285000
  • symbol: JSNZ_001996
  • pan gene symbol?: scrR
  • synonym:
  • product: LacI family DNA-binding transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001996
  • symbol: JSNZ_001996
  • product: LacI family DNA-binding transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 2009360..2010310
  • length: 951
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAAATATATCTGATATTGCCAAATTGGCAGGCGTTTCAAAAAGTACAGTATCTAGA
    TTTTTAAATAATGGATCTGTCAGTAAAAAAACAAGTGAAAAATTAACAAAAATTATAGCA
    GAACATGACTATCAACCGAATCAATTTGCTCAAAGTTTAAGAGCGAGACAAACACACCTT
    ATTGGTGCCATTATACCTAGAATGAATTCATATGCAGTAGACGAAACAATCAAAGGATTG
    GCAAAACAATGCCAAAAATATGAATCACAATTAATTTTAAATTATACAGGTTTAAATATC
    GAAGCAGAAATACAAGCGCTTGAAACATTAGCACGCAGTAAAGTAGATGGTATTGTTTTA
    ATGGCTACAGATATAACAGAGAGACATATTGAAGTCATTAATAAAATGAATGTACCAATC
    GTTATTGTTGGTCAACAACATGAACAACTTCATAGTATTGTGCATGATGATTATAAAGCA
    GGTCAAATTATAGGCGAATGGATTGGTCAACAGGGATATCAACAAGTTGAAGTGTTTAGC
    GTAAGTGAAAAAGATATTGCAGTTGGTATACATAGAAAACGTGGTTTACTTGACCAGTTA
    GCTAAATACCAAATTAAACCTAATATACATGAAACAAATTTTACTTATGTGGAAGCACAA
    AAAGATGTTGCAAATGTTTTGGAAAATGTGGAGCAAGTAGATGCGGTTGTTGGAGCAACT
    GATACGATTGCATTAGCTGCCTATAAATATTATTCTGATAAAAAAGATGTTATGAAACCA
    CATCAAATATATGGTTTTGGTGGTGACCCAATGACACAATTAGTGTCTCCATCGATAAAA
    ACAATTCATTATAATTATTTTGAAGCTGGCCAATGCGCGATGGAAGAGATACAACAGATG
    CTTAAAAAGCAAGATATGCCATATAGCGTCACAGTAGATGTTAATATTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    951

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001996
  • symbol: JSNZ_001996
  • description: LacI family DNA-binding transcriptional regulator
  • length: 316
  • theoretical pI: 6.6818
  • theoretical MW: 35471.3
  • GRAVY: -0.295886

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 150.8)
    and 3 more
    Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 119.9)
    Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 114.3)
    CRISPR-associated endonuclease Cas1 (TIGR00287; EC 3.1.-.-; HMM-score: 15.7)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Periplas_BP (CL0144) Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 66.9)
    HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 62.7)
    and 22 more
    Periplas_BP (CL0144) Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 49.1)
    Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 26.2)
    HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 20.4)
    Peptidase_CA (CL0125) Vasohibin; Vasohibin (PF14822; HMM-score: 19.4)
    HTH (CL0123) HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 16.9)
    HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 15.4)
    DUF3853; Protein of unknown function (DUF3853) (PF12964; HMM-score: 14.7)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 14.7)
    HTH_6; Helix-turn-helix domain, rpiR family (PF01418; HMM-score: 14.6)
    HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 14.3)
    Xre-like-HTH; Antitoxin Xre-like helix-turn-helix domain (PF20432; HMM-score: 13.9)
    HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 13.8)
    HTH_10; HTH DNA binding domain (PF04967; HMM-score: 13.3)
    MarR; MarR family (PF01047; HMM-score: 13.2)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 13.2)
    HTH_29; Winged helix-turn helix (PF13551; HMM-score: 13)
    TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 12.9)
    HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 12.3)
    HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 12.1)
    HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 12)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 12)
    GAP (CL0409) Msb1-Mug8_dom; Meiotically up-regulated protein Msb1/Mug8 domain (PF08101; HMM-score: 11.6)

⊟Structure, modifications & cofactors[edit | edit source]

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9994
    • Cytoplasmic Membrane Score: 0.0003
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0003
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010492
    • TAT(Tat/SPI): 0.001586
    • LIPO(Sec/SPII): 0.001704
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTKIIAEHDYQPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEAEIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKAGQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVEAQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVSPSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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