PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001932
• pan locus tag^?: SAUPAN004927000
• symbol: gatA
• pan gene symbol^?: gatA
• synonym:
• product: Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001932
• symbol: gatA
• product: Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA
• replicon: chromosome
• strand: -
• coordinates: 1945267..1946724
• length: 1458
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGAGCATTCGCTACGAATCGGTTGAGAATTTATTAACTTTAATAAAAGACAAAAAAATC
  AAACCATCTGATGTTGTTAAAGATATATATGATGCAATTGAAGAGACTGATCCAACAATT
  AAGTCTTTTCTAGCGCTGGATAAAGAAAATGCAATCAAAAAAGCGCAAGAATTGGATGAA
  TTACAAGCAAAAGATCAAATGGATGGCAAATTATTTGGTATTCCAATGGGTATAAAAGAT
  AACATTATTACAAACGGATTAGAAACAACATGTGCAAGTAAAATGTTAGAAGGTTTTGTG
  CCAATTTACGAATCTACTGTAATGGAAAAACTACATAAAGAGAATGCCGTTTTAATCGGT
  AAATTAAACATGGATGAGTTTGCAATGGGTGGTTCAACAGAAACATCTTATTTCAAAAAA
  ACAGTTAACCCATTTGACCATAAAGCAGTACCAGGTGGTTCATCAGGTGGATCTGCAGCA
  GCAGTTGCAGCTGGCTTAGTACCATTTAGCTTAGGTTCAGACACAGGTGGTTCAATTAGA
  CAACCGGCTGCATATTGTGGCGTTGTCGGTATGAAACCAACATACGGTCGTGTATCTCGA
  TTTGGATTAGTTGCTTTTGCATCTTCATTAGACCAAATTGGTCCATTGACTCGAAATGTA
  AAAGATAATGCAATCGTATTAGAAGCTATTTCTGGTGCAGATGTTAATGACTCTACAAGT
  GCACCAGTTGATGATGTAGACTTTACATCTGAAATTGGTAAAGATATTAAAGGATTAAAA
  GTTGCATTACCTAAAGAATACTTAGGTGAAGGTGTAGCTGATGACGTAAAAGAAGCAGTT
  CAAAACGCTGTAGAAACTTTAAAATCTTTAGGTGCTGTCGTTGAGGAAGTATCATTGCCA
  AATACTAAATTTGGTATTCCATCATATTACGTGATTGCATCATCAGAAGCTTCGTCAAAC
  CTTTCTCGTTTTGACGGAATTCGTTATGGTTATCATTCTAAAGAAGCTCATTCATTAGAA
  GAATTATATAAAATGTCAAGATCTGAAGGTTTCGGTAAAGAAGTAAAACGTCGTATTTTC
  TTAGGTACATTTGCATTAAGTTCAGGTTACTATGATGCTTACTATAAAAAATCTCAAAAA
  GTTAGAACATTGATTAAAAATGACTTTGATAAAGTATTCGAAAATTATGATGTAGTAGTT
  GGTCCAACAGCGCCTACAACTGCGTTTAATTTAGGTGAAGAAATTGATGATCCATTAACA
  ATGTATGCCAATGATTTATTAACAACACCAGTAAACTTAGCTGGATTACCTGGTATTTCT
  GTTCCTTGTGGACAATCAAATGGCCGACCAATCGGTTTACAGTTCATTGGTAAACCATTC
  GATGAAAAAACGTTATATCGTGTCGCTTATCAATATGAAACACAATACAATTTACATGAC
  GTTTATGAAAAATTATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1458
  

⊟Protein

⊟General

• locus tag: JSNZ_001932
• symbol: GatA
• description: Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA
• length: 485
• theoretical pI: 4.81653
• theoretical MW: 52834.5
• GRAVY: -0.235464

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis tRNA aminoacylation aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (TIGR00132; EC 6.3.5.-; HMM-score: 657.7)
  and 2 more
  Unknown function Enzymes of unknown specificity amidohydrolase, AtzE family (TIGR02715; EC 3.-.-.-; HMM-score: 308.7)
  Metabolism Central intermediary metabolism Nitrogen metabolism allophanate hydrolase (TIGR02713; EC 3.5.1.54; HMM-score: 213.5)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  no clan defined Amidase; Amidase (PF01425; HMM-score: 522)
  and 1 more
  Thioredoxin (CL0172) Calsequestrin; Calsequestrin (PF01216; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8913
  • Cytoplasmic Membrane Score: 0.0216
  • Cell wall & surface Score: 0.0031
  • Extracellular Score: 0.084
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002346
  • TAT(Tat/SPI): 0.000217
  • LIPO(Sec/SPII): 0.000614
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFLALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPIYESTVMEKLHKENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAAAVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTRNVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDVKEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSKEAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKVFENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRPIGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications