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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001831
  • pan locus tag?: SAUPAN004758000
  • symbol: hemE
  • pan gene symbol?: hemE
  • synonym:
  • product: uroporphyrinogen decarboxylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001831
  • symbol: hemE
  • product: uroporphyrinogen decarboxylase
  • replicon: chromosome
  • strand: -
  • coordinates: 1869557..1870594
  • length: 1038
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGTGCATAATAAAAACAATACAATTTTAAAAATGATCAAAGGTGAAGAAACATCACAT
    ACACCTGTTTGGTTTATGCGACAAGCTGGCCGTTCGCAACCAGAATATCGAAAATTGAAA
    GAAAAATATTCACTATTCGATATTACACATCAGCCGGAGTTGTGCGCTTATGTAACACAT
    TTACCAGTTGATAATTATCATACAGATGCAGCAATTTTATACAAAGATATTATGACACCA
    TTAAAGCCAATTGGTGTCGATGTAGAAATTAAATCGGGTATTGGTCCAGTGATTCATAAT
    CCAATCAAAACAATTCAAGATGTTGAGAAACTTTCTCAAATAGACCCCGAACGAGATGTA
    CCATATGTATTAGATACAATTAAACTTTTAACAGAAGAAAAGTTAAATGTGCCGCTAATA
    GGATTTACTGGGGCACCATTTACATTAGCGTCATATATGATTGAAGGCGGACCATCGAAA
    AATTACAATTTTACAAAAGCGATGATGTATAGAGATGAAGCAACATGGTTTGCTTTAATG
    AATCATTTAGTTGATGTATCTGTTAAATATGTAACAGCTCAAGTCGAAGCAGGTGCCGAA
    TTGATTCAAATTTTCGATTCATGGGTAGGTGCATTAAATGTCGAGGATTACAGACGTTAC
    ATTAAACCACATATGATTCGATTAATCAGTGAGGTTAAAGAAAAACATGATGTGCCGGTA
    ATTTTATTCGGTGTAGGTGCCAGTCATTTAATCAATGAATGGAATGATTTACCGATTGAT
    GTATTAGGCCTAGATTGGAGAACGTCTATTAATCAGGCTCAACAATTAGGCGTTACTAAA
    ACATTACAAGGGAATTTAGATCCATCAATTTTATTAGCACCATGGAATGTCATTGAAGAG
    AGATTGAAACCAATATTAGATCAAGGTATGGAGAACGGTAAACACATCTTTAATTTAGGA
    CACGGTGTATTCCCAGAAGTGCAACCAGAGACGTTACGTAAAGTAAGCGAATTCGTACAC
    ACATATACACAAAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1038

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001831
  • symbol: HemE
  • description: uroporphyrinogen decarboxylase
  • length: 345
  • theoretical pI: 6.324
  • theoretical MW: 39352.1
  • GRAVY: -0.231304

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.1.37?  ExPASy
    Uroporphyrinogen decarboxylase Uroporphyrinogen III = coproporphyrinogen + 4 CO2
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin uroporphyrinogen decarboxylase (TIGR01464; EC 4.1.1.37; HMM-score: 420.6)
    and 2 more
    methyltransferase, MtaA/CmuA family (TIGR01463; EC 2.1.1.-; HMM-score: 61.8)
    Signal transduction Regulatory functions Protein interactions phosphohistidine phosphatase SixA (TIGR00249; EC 3.1.3.-; HMM-score: 15.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) URO-D; Uroporphyrinogen decarboxylase (URO-D) (PF01208; HMM-score: 418.3)
    and 1 more
    TPR (CL0020) COPI_C; Coatomer (COPI) alpha subunit C-terminus (PF06957; HMM-score: 10.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9928
    • Cytoplasmic Membrane Score: 0.0007
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0061
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006385
    • TAT(Tat/SPI): 0.000484
    • LIPO(Sec/SPII): 0.000852
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYSLFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPIKTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSKNYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYRRYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQLGVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRKVSEFVHTYTQR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: PerR (repression) regulon
    PerR(TF)important in Oxidative stress response;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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