PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001422
• pan locus tag^?: SAUPAN003892000
• symbol: tdcB
• pan gene symbol^?: ilvA1
• synonym:
• product: bifunctional threonine ammonia-lyase/L-serine ammonia-lyase TdcB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001422
• symbol: tdcB
• product: bifunctional threonine ammonia-lyase/L-serine ammonia-lyase TdcB
• replicon: chromosome
• strand: -
• coordinates: 1466044..1467084
• length: 1041
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  
  ATGACAACCAACACAGTTACATTACAAACAGCACATATTGTAAGTTTAGGAGATATCGAA
  GAAGCTAAAGCAAGCATTAAACCATTTATTCGTCGAACACCTCTAATTAAATCAATGTAT
  TTAAGCCAAAGTATAACTAAAGGGAATGTATTTCTAAAATTAGAAAATATGCAATTCACA
  GGATCTTTTAAATTTAGAGGCGCTAGCAATAAAATTAATCATTTGTCAGATGAACAAAAA
  GCTAAAGGTATTATTGGCGCATCAGCCGGAAATCATGCACAAGGTGTAGCTTTGACAGCA
  AAACTATTAGGTATTGATGCAACGATTGTGATGCCAGAAACAGCACCAATTGCTAAACAG
  AATGCTACCAAAGGATATGGTGCAAAAGTCATTTTAAAAGGTAAAAACTTTAACGAAACT
  AGACTTTATATGGAAGAATTAGCGAAAGAAAATGGCATGACAATCGTTCATCCATATGAC
  GATAAGTTTGTCATGGCTGGTCAAGGAACAATTGGTTTAGAAATTTTAGATGATATTTGG
  AATGTGAATACAGTCATCGTACCAGTTGGCGGTGGAGGATTAATTGCAGGTATTGCCACC
  GCATTAAAATCATTTAACCCTTCAATTCATATTATCGGTGTTCAATCTGAGAATGTTCAT
  GGTATGACTGAGTCTTTCTATAAGAGAGATTTAACTGAACATCGAGTGGATAGCACGATA
  GCAGATGGTTGTGATGTAAAAGTTCCTGGTGAACAAACATATGAAGTAGTTAAACATTTA
  GTAGATGAATTTATTCTTGTTACTGAAGAAGAAATTGAACATGCTATGAAAGATTTAATG
  CAGCGTGCCAAAATTATTACTGAAGGTGCAGGCGCATTACCAACAGCTGCAATTTTAAGT
  GGGAAAATTGATAAAAAATGGCTAGAGGGTAAAAATGTCGTTGCATTAGTTTCAGGAGGA
  AACGTTGACTTAACTAGAGTTTCTGGTGTCATTGAACATGGCTTAAATATCGCGGATACA
  AGTAAGGGTGTGGTAGGTTAA
  60
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  420
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  1020
  1041
  

⊟Protein

⊟General

• locus tag: JSNZ_001422
• symbol: TdcB
• description: bifunctional threonine ammonia-lyase/L-serine ammonia-lyase TdcB
• length: 346
• theoretical pI: 6.86975
• theoretical MW: 37177.5
• GRAVY: -0.0251445

⊟Function

• TIGRFAM:
  Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase (TIGR01127; EC 4.3.1.19; HMM-score: 397.8)
  and 16 more
  Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase, biosynthetic (TIGR01124; EC 4.3.1.19; HMM-score: 299.3)
  Metabolism Amino acid biosynthesis Pyruvate family threonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 264.8)
  ectoine utilization protein EutB (TIGR02991; HMM-score: 263)
  Metabolism Amino acid biosynthesis Serine family cysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 92.4)
  Metabolism Amino acid biosynthesis Serine family cysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 81.5)
  Metabolism Amino acid biosynthesis Aspartate family threonine synthase (TIGR00260; EC 4.2.3.1; HMM-score: 75.2)
  Metabolism Amino acid biosynthesis Serine family cysteine synthase B (TIGR01138; EC 2.5.1.47; HMM-score: 68)
  Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnA (TIGR03945; HMM-score: 65.9)
  Metabolism Amino acid biosynthesis Serine family cystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 58.5)
  diaminopropionate ammonia-lyase (TIGR03528; EC 4.3.1.15; HMM-score: 53.6)
  Metabolism Energy metabolism Other diaminopropionate ammonia-lyase family (TIGR01747; EC 4.3.1.15; HMM-score: 46.5)
  pyridoxal phosphate-dependent enzymes, D-cysteine desulfhydrase family (TIGR01275; HMM-score: 31.6)
  Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, beta subunit (TIGR00263; EC 4.2.1.20; HMM-score: 28.9)
  Unknown function Enzymes of unknown specificity pyridoxal-phosphate dependent TrpB-like enzyme (TIGR01415; HMM-score: 27.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteate synthase (TIGR03844; EC 2.5.1.76; HMM-score: 17.4)
  Metabolism Energy metabolism Methanogenesis cysteate synthase (TIGR03844; EC 2.5.1.76; HMM-score: 17.4)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 276.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9415
  • Cytoplasmic Membrane Score: 0.0047
  • Cell wall & surface Score: 0.0005
  • Extracellular Score: 0.0533
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016412
  • TAT(Tat/SPI): 0.000648
  • LIPO(Sec/SPII): 0.001021
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQSITKGNVFLKLENMQFTGSFKFRGASNKINHLSDEQKAKGIIGASAGNHAQGVALTAKLLGIDATIVMPETAPIAKQNATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYDDKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSENVHGMTESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAMKDLMQRAKIITEGAGALPTAAILSGKIDKKWLEGKNVVALVSGGNVDLTRVSGVIEHGLNIADTSKGVVG

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications