From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001421
  • pan locus tag?: SAUPAN003891000
  • symbol: JSNZ_001421
  • pan gene symbol?: —
  • synonym:
  • product: APC family permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001421
  • symbol: JSNZ_001421
  • product: APC family permease
  • replicon: chromosome
  • strand: -
  • coordinates: 1464691..1466013
  • length: 1323
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCAAATGGTAAAGAATTACAAAAAAATATAGGTTTCTTCTCAGCGTTTGCTATTGTT
    ATGGGGACAGTTATTGGTTCAGGAGTATTCTTTAAAATATCAAACGTAACAGAAGTAACA
    GGAACAGCAGGAATGGCCTTGTTTGTATGGTTCCTAGGCGGCATCATTACCATTTGTGCG
    GGGTTAACAGCAGCAGAACTTGCTGCTGCAATCCCTGAAACAGGTGGCTTAACGAAGTAT
    ATAGAATATACATACGGTGATTTCTGGGGCTTCCTATCAGGTTGGGCGCAATCATTTATT
    TATTTTCCAGCTAACGTAGCAGCATTGTCTATCGTATTTGCGACACAGCTAATTAATTTA
    TTCCATTTATCTATAGGTTCGTTAATACCAATAGCAATCGCATCTGCGTTATCTATTGTG
    TTGATAAATTTCCTAGGTTCAAAAGCAGGCGGAATTTTACAATCAGTTACATTAGTAATT
    AAACTGATTCCAATCATCGTAATCGTAATTTTTGGTATTTTTCAATCTGGAGATATCACT
    TTTTCATTAATTCCAACTACAGGTAATTCAGGAAATGGTTTCTTTACAGCAATTGGTAGT
    GGTTTATTAGCAACTATGTTTGCATATGATGGTTGGATTCATGTAGGAAATGTTGCGGGG
    GAACTTAAAAATCCTAAACGCGATTTACCTTTAGCGATTTCAGTTGGTATCGGTTGTATT
    ATGGCTGTGTATTTATTAATTAACGCTACATTCTTATTAACACTGCCAATAGAACTACTT
    GCTGGTAATTTAAATGCAGCTTCAGATACATCAAAAACATTATTTGGTGAAAATGGCGGT
    AAGATTATTACAATCGGTATATTAATTTCTGTTTATGGTACGATCAATGGCTATACTATG
    ACTGGTATGCGCGTACCATATGCAATGGCTGAAAGAAAATTATTGCCATTTAGCCACTTA
    TTCGCAAAATTAACAAAATCTGGCGCACCATGGTTTGGCGCAATTATCCAACTTATAATC
    GCTATCATCATGATGTCAATGGGAGCATTTGATACGATTACAAATATGTTAATCTTCGTA
    ATTTGGTTGTTCTATTGTATGTCATTTGTAGCGGTAATTATTTTAAGAAAACGTGAGCCA
    AATATGGAGCGACCATATAAAGTACCGTTATATCCGATCATACCTTTAATTGCTATTTTG
    GCAGGATCATTTGTATTAATTAATACACTGTTTACACAATTTGTATTAGCAATCATTGGA
    ATTCTAATCACAGCACTTGGTATACCAGTTTATTACTATAAGAAGAAACAAAAAGCAGCA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1323

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001421
  • symbol: JSNZ_001421
  • description: APC family permease
  • length: 440
  • theoretical pI: 9.55367
  • theoretical MW: 47235.1
  • GRAVY: 0.923182

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines L-type amino acid transporter (TIGR00911; HMM-score: 212)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 172.6)
    and 10 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 134.2)
    Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 114.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 87.6)
    Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 72.7)
    putrescine-ornithine antiporter (TIGR04299; HMM-score: 63.4)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (TIGR00907; HMM-score: 60.3)
    histidine-histamine antiporter (TIGR04298; HMM-score: 54.4)
    Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 49.5)
    putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 39.1)
    early transcribed membrane protein (ETRAMP) family (TIGR01495; HMM-score: 8.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 207.8)
    and 2 more
    AA_permease; Amino acid permease (PF00324; HMM-score: 158.8)
    AA_permease_C; C-terminus of AA_permease (PF13906; HMM-score: 36.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9997
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0002
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012697
    • TAT(Tat/SPI): 0.000621
    • LIPO(Sec/SPII): 0.01798
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAGMALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYFPANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVIKLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNVAGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKTLFGENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGAIIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYPIIPLIAILAGSFVLINTLFTQFVLAIIGILITALGIPVYYYKKKQKAA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, CodY (repression) regulon
    Rex*(TF)important in Energy metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_001421&oldid=107062"