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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001423
  • pan locus tag?: SAUPAN003893000
  • symbol: ald
  • pan gene symbol?: ald1
  • synonym:
  • product: alanine dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001423
  • symbol: ald
  • product: alanine dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 1467179..1468297
  • length: 1119
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGTTAGTTGCAGTAGTCAAAGAATTAAAACAAGGTGAAGGACGTGTAGCTTGCACACCC
    GAAAATGTGCGTAAGTTAACGGATGCAGGACATAAAGTAATTGTTGAAAAAAATGCTGGC
    ATTGGTTCAGGATTTTCTAACGATATGTATGAAAAAGAAGGGGCTAAGATCGTAACTCAT
    GAACAAGCATGGGAAGCTGATCTTGTTATCAAAGTAAAAGAACCTCATGAAAGCGAATAT
    CAATATTTCAAAAAAAATCAAATTATCTGGGGATTTTTACATTTAGCATCTTCAAAAGAA
    ATAGTAGAAAAAATGCAAGAAGTTGGTGTAACTGCGATTAGTGGTGAAACCATTATAAAA
    AATGGAAAAGCAGAATTATTAGCGCCAATGAGTGCTATAGCAGGTCAACGCTCAGCAATT
    ATGGGAGCTTACTACTCTGAAGCACAACATGGTGGTCAAGGTACTTTAGTGACTGGTGTA
    CATGAAAATGTGGATATACCTGGTAGTACATATGTGATTTTCGGTGGTGGCGTAGCAGCA
    ACAAATGCAGCAAATGTTGCCTTGGGACTAAATGCTAAAGTAATCATTATCGAGTTAAAT
    GATGACCGCATTAAATATCTTGAAGATATGTATGCAGAAAAAGATGTCACAGTAGTCAAA
    TCAACACCAGAAAATTTAGCAGAACAAATTAAGAAAGCAGATGTATTTATTTCTACAATT
    TTAATTCCAGGTGCGAAACCGCCAAAATTGGTTACTCGTGAGATGGTTAAATCAATGAAA
    AAAGGTTCAGTATTAATCGATATAGCTATTGACCAAGGTGGAACTATTGAAACAATTAGA
    CCAACTACAATTTCTGATCCAGTGTATGAAGAAGAAGGTATGATTCATTATGGTGTACCA
    AATCAACCAGGAGCAGTCCCAAGAACTTCAACAATGGCATTAGCACAAGGAAATATTGAT
    TATATATTAGAAATTTGTGACAAAGGCTTAGAACAAGCAATTAAAGATAATGAAGCCTTA
    AGTACTGGTGTAAACATTTACCAAGGACAAGTGACAAATCAAGGATTAGCTTCATCACAT
    GACCTAGATTATAAAGAAATATTAAATGTTATCGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1119

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001423
  • symbol: Ald
  • description: alanine dehydrogenase
  • length: 372
  • theoretical pI: 4.89305
  • theoretical MW: 40266.7
  • GRAVY: -0.146505

⊟Function[edit | edit source]

  • reaction:
    EC 1.4.1.1?  ExPASy
    Alanine dehydrogenase L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines alanine dehydrogenase (TIGR00518; EC 1.4.1.1; HMM-score: 394)
    and 6 more
    Metabolism Energy metabolism Electron transport NAD(P)(+) transhydrogenase (AB-specific), alpha subunit (TIGR00561; EC 1.6.1.2; HMM-score: 139.7)
    Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 23)
    Metabolism Energy metabolism Amino acids and amines adenosylhomocysteinase (TIGR00936; EC 3.3.1.1; HMM-score: 18.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 16.6)
    methylmalonyl-CoA mutase C-terminal domain (TIGR00640; HMM-score: 15.4)
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate-5-dehydrogenase (TIGR01809; EC 1.1.1.25; HMM-score: 15)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 257.7)
    and 12 more
    AlaDh_PNT_N; Alanine dehydrogenase/PNT, N-terminal domain (PF05222; HMM-score: 146.4)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 24.8)
    THF_DHG_CYH_C; Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (PF02882; HMM-score: 23.7)
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 22.8)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 17.3)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 16.6)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 16.6)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 15.4)
    ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 14.6)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 14.4)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 14.1)
    2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9552
    • Cytoplasmic Membrane Score: 0.0041
    • Cell wall & surface Score: 0.0008
    • Extracellular Score: 0.0399
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011205
    • TAT(Tat/SPI): 0.000954
    • LIPO(Sec/SPII): 0.005196
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSGFSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIVEKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGVHENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTVVKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTIETIRPTTISDPVYEEEGMIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAIKDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Rex* (repression) regulon
    Rex*(TF)important in Energy metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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