PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001403
• pan locus tag^?: SAUPAN003843000
• symbol: JSNZ_001403
• pan gene symbol^?: murG
• synonym:
• product: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001403
• symbol: JSNZ_001403
• product: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase
• replicon: chromosome
• strand: -
• coordinates: 1418564..1419634
• length: 1071
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  
  ATGACGAAAATCGCATTTACCGGAGGGGGAACAGTTGGACACGTATCAGTAAATTTAAGT
  TTAATTCCAACTGCATTATCACAAGGTTATGAAGCGCTTTATATTGGTTCTAAAAATGGT
  ATTGAAAGAGAAATGATTGAATCACAACTACCAGAAATTAAGTATTATCCTATTTCGAGT
  GGTAAATTAAGAAGATATATTTCTTTAGAAAATGCCAAAGACGTATTTAAAGTATTGAAA
  GGTATTCTTGATGCTCGTAAAGTTTTGAAAAAAGAAAAACCTGATCTATTATTTTCAAAA
  GGTGGATTTGTATCTGTGCCTGTTGTTATTGCAGCCAAATCATTAAATATACCAACTATT
  ATTCATGAATCTGACTTAACACCAGGATTAGCGAATAAGATAGCACTTAAATTTGCCAAG
  AAAATATATACAACATTTGAAGAAACGCTAAACTACTTACCTAAAGAGAAAGCTGATTTT
  ATTGGAGCAACAATTCGAGAAGATTTAAAAAATGGTAATGCACATAATGGTTATCAATTA
  ACAGGCTTTAATGAAAATAAAAAAGTTTTACTCGTTATGGGTGGAAGCTTAGGAAGTAAA
  AAATTAAATAGCATTATTCGCGAAAACTTAGATGCATTATTACAACAATATCAAGTGATA
  CATTTAACTGGTAAAGGATTAAAAGATGCTCAAGTTAAAAAATCAGGATATATACAATAT
  GAATTTGTTAAAGAGGATTTAACAGATTTATTAGCAATTACGGACACAGTAATAAGTAGA
  GCAGGATCAAATGCGATTTATGAGTTCTTAACATTACGTATACCAATGTTATTAGTACCA
  TTAGGTTTAGATCAATCCCGAGGCGACCAAATTGACAATGCAAATCATTTTGCTGATAAA
  GGTTATGCTAAAGCGATTGATGAAGAACAATTAACAGCACAAATTTTATTACAAGAACTA
  AATGAAATGGAACAGGAAAGAACTCGAATTATCAATAATATGAAATCGTATGAACAAAGT
  TATACGAAAGAAGCTTTATTTGATAAGATGATTAAAGATGCATTGAATTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1071
  

⊟Protein

⊟General

• locus tag: JSNZ_001403
• symbol: JSNZ_001403
• description: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase
• length: 356
• theoretical pI: 9.13983
• theoretical MW: 39696.5
• GRAVY: -0.227528

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (TIGR01133; EC 2.4.1.227; HMM-score: 244.5)
  and 2 more
  Cellular processes Cellular processes Toxin production and resistance glycosyltransferase, MGT family (TIGR01426; EC 2.4.1.-; HMM-score: 41.5)
  Hypothetical proteins Conserved conserved hypothetical protein (TIGR00661; HMM-score: 31.6)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  GT-B (CL0113) Glyco_transf_28; Glycosyltransferase family 28 N-terminal domain (PF03033; HMM-score: 129.9)
  and 6 more
  Glyco_tran_28_C; Glycosyltransferase family 28 C-terminal domain (PF04101; HMM-score: 97.5)
  UDPGT; UDP-glucoronosyl and UDP-glucosyl transferase (PF00201; HMM-score: 29.7)
  Glyco_trans_1_3; Glycosyl transferase family 1 (PF13528; HMM-score: 19.1)
  Glyco_trans_4_4; Glycosyl transferase 4-like domain (PF13579; HMM-score: 14.9)
  Glyco_transf_4; Glycosyltransferase Family 4 (PF13439; HMM-score: 14.7)
  no clan defined Mistic; Membrane-integrating protein Mistic (PF11458; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.05
  • Cytoplasmic Membrane Score: 8.78
  • Cellwall Score: 0.08
  • Extracellular Score: 0.09
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.1231
  • Cytoplasmic Membrane Score: 0.8596
  • Cell wall & surface Score: 0.0015
  • Extracellular Score: 0.0158
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.310983
  • TAT(Tat/SPI): 0.006498
  • LIPO(Sec/SPII): 0.013024
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIEREMIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGGFVSVPVVIAAKSLNIPTIIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADFIGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQVIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPMLLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNMKSYEQSYTKEALFDKMIKDALN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications