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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001397
  • pan locus tag?: SAUPAN003831000
  • symbol: JSNZ_001397
  • pan gene symbol?: sucA
  • synonym:
  • product: 2-oxoglutarate dehydrogenase E1 component

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001397
  • symbol: JSNZ_001397
  • product: 2-oxoglutarate dehydrogenase E1 component
  • replicon: chromosome
  • strand: -
  • coordinates: 1412052..1414850
  • length: 2799
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACTAACGAAAGAAAAGAAGTTTCAGAGGCTCCTGTAAACTTCGGTGCGAATTTAGGT
    CTAATGTTAGATCTATATGATGACTTTTTACAAGATCCATCGTCTGTACCAGAAGATTTA
    CAAGTCTTATTCAGCACAATTAAGAATGATGACTCAATTGTACCAGCTTTAAAAAGTACA
    AGTAGTCAAAATAGCGACGGCACAATTAAGCGTGTCATGCGTTTAATTGATAATATTCGC
    CAATACGGGCATCTTAAAGCCGATATTTATCCTGTAAATCCTCCAAAAAGGAAACATGTA
    CCTAAATTAGAGATTGAAGACTTTGATTTAGATCAACAGATTTTGGAAGGTATATCAGCA
    GGAATTGTTTCAGATCACTTTGCCGACATTTATGATAATGCTTATGAAGCAATTTTAAGA
    ATGGAAAAACGTTACAAAGGACCAATTGCATTTGAGTATACACATATTAATAACAATACC
    GAACGTGGTTGGTTAAAAAGAAGAATTGAAACGCCATATAAAGTAACGTTAAATAATAAC
    GAAAAAAGGGCACTATTCAAACAATTAGCGTATGTTGAAGGGTTTGAAAAATATCTTCAT
    AAAAACTTCGTTGGTGCAAAGCGTTTTTCAATTGAAGGGGTAGACGCACTTGTACCGATG
    TTACAACGTACTATTACGATTGCTGCGAAAGAAGGTATTAAAAATATACAAATAGGCATG
    GCTCACCGTGGACGTTTAAACGTTTTAACGCATGTCTTAGAAAAACCGTACGAAATGATG
    ATTTCAGAATTTATGCATACAGATCCAATGAAATTCTTACCTGAAGATGGTAGCTTGCAG
    TTAACTGCTGGATGGACTGGTGATGTGAAATATCACCTTGGTGGCATTAAAACTACTGAT
    TCATACGGTACAATGCAGCGTATTGCACTGGCTAACAATCCAAGTCACTTGGAAATTGTT
    GCACCTGTTGTTGAGGGGCGTACGAGAGCAGCACAAGATGATACACAACGTGCTGGGGCT
    CCGACGACTGATCATCATAAAGCAATGCCAATTATTATACATGGCGATGCTGCTTATCCT
    GGTCAAGGAATTAACTTCGAAACAATGAACTTAGGAAACTTGAAAGGCTATTCTACGGGT
    GGTTCATTGCATATTATTACTAACAATAGAATTGGATTTACTACAGAACCAATTGATGCA
    CGTTCAACAACTTATTCTACAGATGTGGCCAAAGGTTATGATGTGCCAATATTCCATGTC
    AATGCTGATGACGTTGAAGCTACTATTGAAGCAATTGATATTGCAATGGAATTTAGAAAA
    GAGTTTCATAAAGACGTCGTTATTGATTTAGTAGGTTATCGTCGTTTCGGTCATAACGAA
    ATGGATGAACCATCAATTACTAATCCAGTTCCTTATCAGAATATTCGCAAACATGACTCT
    GTTGAATATGTGTTTGGTAAAAAGCTTGTTAATGAAGGTGTCATTTCAGAAGATGAAATG
    CATTCATTTATAGAACAAGTCCAAAAGGAACTAAGACAAGCACATGATAAAATTAATAAA
    GCTGATAAAATGGATAATCCAGATATGGAAAAACCTGCAGAACTTGCATTACCGTTACAA
    GCAGACGAACAATCATTTACGTTTGATCATTTGAAAGAAATAAATGATGCATTGTTAACA
    TATCCGGATGGCTTTAACATTTTGAAAAAGTTAAACAAAGTTCTTGAGAAGCGTCATGAG
    CCGTTTAATAAAGAAGATGGTTTAGTTGATTGGGCACAAGCAGAACAACTTGCATTTGCG
    ACAATTTTACAAGATGGTACACCGATTCGCTTAACTGGTCAAGATAGTGAACGTGGTACA
    TTCAGTCATAGGCATGCCGTGTTACATGATGAGCAAACAGGTGAAACATATACACCTTTA
    CATCATGTTCCTGATCAAAAAGCGACATTTGATATACACAATTCTCCGCTTTCAGAAGCA
    GCAGTAGTTGGTTTTGAATACGGCTATAATGTGGAAAACAAAAAAAGCTTCAATATTTGG
    GAAGCGCAATATGGTGATTTTGCAAATATGTCACAAATGATTTTTGACAACTTCTTATTC
    AGTTCTCGCTCAAAATGGGGAGAACGTTCAGGATTAACATTATTCTTACCTCATGCATAT
    GAGGGTCAAGGACCTGAACATTCATCAGCAAGATTAGAGCGATTTTTACAATTAGCTGCT
    GAAAATAATTGCACAGTTGTCAACTTATCTAGTTCAAGTAATTATTTCCACTTATTGCGT
    GCACAAGCAGCTAGTTTAGATTCTGAACAAATGCGACCATTGGTTGTTATGTCACCAAAA
    AGCTTATTGAGAAATAAAACAGTTGCCAAACCAATTGATGAGTTTACTTCTGGTGGATTT
    GAGCCAATTTTGACAGAATCATATCAAGCGGATAAGGTTACAAAAGTTATTTTAGCAACT
    GGTAAAATGTTCATTGATTTAAAAGAAGCATTAGCTAAAAATCCAGACGAATCAGTGTTA
    CTCGTTGCGATTGAAAGATTGTATCCATTCCCAGAGGAAGAGATTGAAGCATTACTAGCA
    CAATTGCCAAACCTTGAAGAAGTGTCATGGGTACAAGAAGAACCTAAAAATCAAGGTGCA
    TGGTTATATGTCTATCCATATGTTAAAGTGCTAGTTGCAGATAAATATGATTTAAGTTAT
    CATGGCAGAATTCAAAGGGCTGCTCCAGCTGAAGGCGATGGAGAAATTCATAAACTTGTT
    CAAAATAAAATTATAGAAAATGCATTAAAAAATAACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2799

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001397
  • symbol: JSNZ_001397
  • description: 2-oxoglutarate dehydrogenase E1 component
  • length: 932
  • theoretical pI: 5.40195
  • theoretical MW: 105368
  • GRAVY: -0.464378

⊟Function[edit | edit source]

  • reaction:
    EC 1.2.4.2?  ExPASy
    Oxoglutarate dehydrogenase (succinyl-transferring) 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2
  • TIGRFAM:
    Metabolism Energy metabolism TCA cycle oxoglutarate dehydrogenase (succinyl-transferring), E1 component (TIGR00239; EC 1.2.4.2; HMM-score: 1032.8)
    and 2 more
    Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03182; EC 1.2.4.1; HMM-score: 52.6)
    Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03181; EC 1.2.4.1; HMM-score: 31.3)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    THDP-binding (CL0254) E1_dh; Dehydrogenase E1 component (PF00676; HMM-score: 206.1)
    and 5 more
    Transket_pyr; Transketolase, pyrimidine binding domain (PF02779; HMM-score: 139.8)
    TKC_like (CL0591) OxoGdeHyase_C; 2-oxoglutarate dehydrogenase C-terminal (PF16870; HMM-score: 117.2)
    no clan defined 2-oxogl_dehyd_N; 2-oxoglutarate dehydrogenase N-terminus (PF16078; HMM-score: 21)
    TPR (CL0020) Importin_rep_6; Importin repeat 6 (PF18829; HMM-score: 12.5)
    TKC_like (CL0591) PFOR_II; Pyruvate:ferredoxin oxidoreductase core domain II (PF17147; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.95
    • Cytoplasmic Membrane Score: 0.05
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7439
    • Cytoplasmic Membrane Score: 0.0492
    • Cell wall & surface Score: 0.0031
    • Extracellular Score: 0.2038
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002478
    • TAT(Tat/SPI): 0.000638
    • LIPO(Sec/SPII): 0.000551
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLFSTIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPKLEIEDFDLDQQILEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNTERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALVPMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPEDGSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDDTQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIGFTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDLVGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQKELRQAHDKINKADKMDNPDMEKPAELALPLQADEQSFTFDHLKEINDALLTYPDGFNILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHRHAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEAQYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAAENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSGGFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIEALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDGEIHKLVQNKIIENALKNN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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