PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001396
• pan locus tag^?: SAUPAN003830000
• symbol: sucB
• pan gene symbol^?: sucB
• synonym:
• product: dihydrolipoyllysine-residue succinyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001396
• symbol: sucB
• product: dihydrolipoyllysine-residue succinyltransferase
• replicon: chromosome
• strand: -
• coordinates: 1410782..1412038
• length: 1257
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  
  ATGCCAGAGGTTAAAGTTCCAGAATTAGCAGAATCTATTACAGAAGGTACCATTGCAGAA
  TGGTTGAAAAACGTAGGGGATAGCGTAGAAAAAGGTGAAGCTATTCTTGAATTAGAAACT
  GATAAAGTTAATGTCGAAGTTGTATCTGAAGAAGCAGGTGTATTATCTGAACAACTTGCA
  AGTGAAGGCGACACTGTAGAAGTTGGACAAGCAATTGCTATCATCGGCGAAGGTAGTGGC
  AATGCTTCTAAAGAAAATAGTAACGACAATACTCCACAACAAAATGAAGAAACAAATAAT
  AAAAAAGAAGAAACAACAAATAATTCGGTAGATAAAGCTGAAAATGATGACAATCAGCAA
  CGTATTAATGCTACGCCTTCTGCGCGTCGATATGCTCGTGAAAATGGTGTGAATCTTGCT
  GAAGTAAGTCCGAAAACAAATGATGTGGTTCGTAAAGAAGATATTGATAAGAAACAACAG
  GCACCGGCATCAACACAAACAACACAACAAGCATCTGCAAAAGAAGAAAAAAAATACAAT
  CAATATCCTACAAAACCAGTGATTCGTGAAAAAATGTCACGCAGAAAGAAAACAGCTGCC
  AAAAAATTATTAGAGGTATCTAATAATACAGCTATGTTAACAACATTTAACGAAGTTGAC
  ATGACAAATGTTATGGAATTGCGTAAACGTAAGAAAGAACAATTTATGAAAGATCATGAT
  GGTACTAAATTAGGATTTATGTCATTCTTTACTAAAGCTTCTGTAGCAGCTTTGAAAAAG
  TATCCAGAAGTTAATGCAGAAATCGATGGCGACGACATGATTACGAAACAATATTATGAT
  ATTGGTGTAGCTGTTTCTACAGATGATGGATTATTAGTACCATTTGTAAGAGATTGTGAT
  AAAAAGAATTTTGCAGAAATCGAAGCAGAAATTGCTAATTTAGCAGTTAAAGCACGAGAG
  AAAAAACTTGGCTTAGATGATATGGTTAATGGTTCATTTACGATTACAAATGGCGGTATT
  TTTGGATCAATGATGAGTACGCCAATTATCAATGGTAATCAAGCTGCAATCTTAGGCATG
  CATTCAATTATTACAAGACCAATTGCGATTGATCAAGATACAATCGAAAATCGTCCAATG
  ATGTATATTGCATTAAGCTATGATCATAGAATTATTGACGGTAAAGAAGCAGTTGGATTC
  TTAAAAACAATTAAAGAATTAATTGAAAACCCAGAAGACTTATTATTAGAATCTTAA
  60
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  720
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  1020
  1080
  1140
  1200
  1257
  

⊟Protein

⊟General

• locus tag: JSNZ_001396
• symbol: SucB
• description: dihydrolipoyllysine-residue succinyltransferase
• length: 418
• theoretical pI: 4.57447
• theoretical MW: 46260.7
• GRAVY: -0.615311

⊟Function

• reaction:
  EC 2.3.1.61?  ExPASy
  Dihydrolipoyllysine-residue succinyltransferase Succinyl-CoA + enzyme N⁶-(dihydrolipoyl)lysine = CoA + enzyme N⁶-(S-succinyldihydrolipoyl)lysine
• TIGRFAM:
  Metabolism Energy metabolism TCA cycle dihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex (TIGR01347; EC 2.3.1.61; HMM-score: 614.1)
  and 11 more
  2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase (TIGR02927; EC 2.3.1.61; HMM-score: 299.5)
  Metabolism Energy metabolism Pyruvate dehydrogenase dihydrolipoyllysine-residue acetyltransferase (TIGR01348; EC 2.3.1.12; HMM-score: 266.1)
  Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase (TIGR01349; EC 2.3.1.12; HMM-score: 243)
  Metabolism Transport and binding proteins Cations and iron carrying compounds oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 22.6)
  Metabolism Energy metabolism Other oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 22.6)
  Metabolism Central intermediary metabolism Nitrogen metabolism urea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 21.4)
  Metabolism Transport and binding proteins Unknown substrate efflux transporter, RND family, MFP subunit (TIGR01730; HMM-score: 16.4)
  amphi-Trp domain (TIGR04354; HMM-score: 14.4)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism thymidylate synthase, flavin-dependent (TIGR02170; EC 2.1.1.148; HMM-score: 8)
  Metabolism Transport and binding proteins Cations and iron carrying compounds potassium uptake protein, Trk family (TIGR00934; HMM-score: 5.6)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, biotin carboxyl carrier protein (TIGR00531; HMM-score: 5.5)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  CoA-acyltrans (CL0149) 2-oxoacid_dh; 2-oxoacid dehydrogenases acyltransferase (catalytic domain) (PF00198; HMM-score: 278.4)
  and 7 more
  Hybrid (CL0105) Biotin_lipoyl; Biotin-requiring enzyme (PF00364; HMM-score: 75.1)
  no clan defined E3_binding; e3 binding domain (PF02817; HMM-score: 29.7)
  Hybrid (CL0105) Biotin_lipoyl_2; Biotin-lipoyl like (PF13533; HMM-score: 20.3)
  PYNP_C; Pyrimidine nucleoside phosphorylase C-terminal domain (PF07831; HMM-score: 14.5)
  HlyD_3; HlyD family secretion protein (PF13437; HMM-score: 13.9)
  no clan defined MC5; ABC-three component (ABC-3C) system Middle Component 5 (PF20291; HMM-score: 11.7)
  Peptidase_AD (CL0130) Presenilin; Presenilin (PF01080; HMM-score: 7.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9946
  • Cytoplasmic Membrane Score: 0.0031
  • Cell wall & surface Score: 0.0003
  • Extracellular Score: 0.0021
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003516
  • TAT(Tat/SPI): 0.000409
  • LIPO(Sec/SPII): 0.000396
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVNVEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKKEETTNNSVDKAENDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDIDKKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLTTFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDDMITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVNGSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYDHRIIDGKEAVGFLKTIKELIENPEDLLLES

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications