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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

organism: Staphylococcus aureus JSNZ
locus tag: JSNZ_001383
pan locus tag^?: SAUPAN003812000
symbol: JSNZ_001383
pan gene symbol^?: alr2
synonym:
product: alanine racemase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: JSNZ_001383
symbol: JSNZ_001383
product: alanine racemase
replicon: chromosome
strand: +
coordinates: 1398543..1399628
length: 1086
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID:
RefSeq:
BioCyc:
MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
TTGACAGCAACATGGTCTGTAAATAAGAAAATATTTTTACAAAATGCAATCACAGTCAAA
AACAATCAGCCATTAATGGCAGTTGTTAAAAATAATGCATATCACTATGACCTAGAATTT
GCTGTAACTCAGTTTATCCATGCAGGTATAGATACATTTAGCACAACATCACTACGAGAA
GCAATTCAAATTAGACAACTTGCTCCAGATGCAACAATCTTTTTAATGAATGCAGTATAC
GAATTTGATTTAGTCCGTGAACATCAAATACACATGACTTTGCCGTCGTTGACATATTAC
TATAACCATAAAAATGATTTAGCTGGTATTCATGTTCACTTAGAATTTGAAAATTTATTA
CATCGGTCTGGATTTAAAGATTTAAACGAAATTAAAGAAGTATTGAAGGATCACCATCAA
AATCAAAATGCAAAAATGATTATTAGTGGTTTATGGACCCATTTTGGATATGCTGATGAA
TTCGATGTGTCAGATTATAATGTTGAACGTTCACAATGGATGGAAATTGTTGAATCACTT
TTATCTGAAGGTTATCAGTTCGACCTAATCCATGCACAAAATAGTGCGAGTTTTTATCGG
GAAGGACAAATATTACTACCCCACCATACACATGCGCGTGTAGGTATTGCGTTATACGGT
TCAAGACCATATAGTTCACTGAATCAACATGATATTGTTCAGTCATTGACAGTAAAAGCA
CATGTTATTCAAGTGCGCGAAGTGCAAGCTGGTGATTATTGCGGTTATAGCTTTGCCTTT
GAAGTGACTAAAAACAATACAAAATTAGCTGTAGTTGATATCGGTTATGGCGATGGAATT
TTAAGAACACGTGCTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCA
TTAATGATGAGCCATATGTTTGTTGAAGTAGATGGCAATGTACATGCACAAGATGAAGTT
ATTCTTTATAATAATGATATCCGCATCGATGAATATACCTTTAAAGGTGTTGGTGCAAAT
TCTGAACAATTAAGTGCTATGAATCATGATTCTTTAAAAAAGGAGTACATTTCAAATGAC
TGTTAA
60
120
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240
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360
420
480
540
600
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840
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1020
1080
1086

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: JSNZ_001383
symbol: JSNZ_001383
description: alanine racemase
length: 361
theoretical pI: 6.38822
theoretical MW: 41402.4
GRAVY: -0.304155

⊟Function[edit | edit source]

TIGRFAM:
Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan alanine racemase (TIGR00492; EC 5.1.1.1; HMM-score: 148.6)
TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
PFAM:
TIM_barrel (CL0036) Ala_racemase_N; Alanine racemase, N-terminal domain (PF01168; HMM-score: 125.3)
and 1 more
ALR_C-like (CL0866) Ala_racemase_C; Alanine racemase, C-terminal domain (PF00842; HMM-score: 66.5)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.89
- Cytoplasmic Membrane Score: 0.09
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9978
- Cytoplasmic Membrane Score: 0.0002
- Cell wall & surface Score: 0
- Extracellular Score: 0.002
LocateP:
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002434
- TAT(Tat/SPI): 0.000132
- LIPO(Sec/SPII): 0.000473
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI:
RefSeq:
UniProt:

⊟Protein sequence[edit | edit source]

MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQFIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYNHKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHQNQNAKMIISGLWTHFGYADEFDVSDYNVERSQWMEIVESLLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIALYGSRPYSSLNQHDIVQSLTVKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGYGDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTFKGVGANSEQLSAMNHDSLKKEYISNDC

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell: data available for COL
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

Operon-mapper ^[1] : JSNZ_001377 > JSNZ_001378 > dapA > dapB > dapD > JSNZ_001382 > JSNZ_001383 > lysA

⊟Regulation[edit | edit source]

regulator: CodY (repression) regulon
CodY (TF) important in Amino acid metabolism; regulation predicted or transferred from N315 and NCTC 8325 ^[2]

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: data available for COL

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
Bioinformatics: 2018, 34(23);4118-4120
[PubMed:29931111] [WorldCat.org] [DOI] (I p)
↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
Curr Res Microb Sci: 2025, 9;100489
[PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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