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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001377
  • pan locus tag?: SAUPAN003806000
  • symbol: JSNZ_001377
  • pan gene symbol?: lysC
  • synonym:
  • product: aspartate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001377
  • symbol: JSNZ_001377
  • product: aspartate kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 1392632..1393837
  • length: 1206
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGTAACAAGAAGTGTGTTGAAATTTGGCGGATCATCCGTCAGTGATTTTACAAAAATA
    AAAAGGATCGCTGAAATGTTAAAGGAGCGAGTCAATCAAGATGAACAATTAATTGTCGTT
    GTAAGTGCTATGGGTAACACAACAGATCAATTAATGACGAATGTATCGACCTTGACTAAA
    GCACCAAAACAACAAGAACTGGCATTATTATTGACAACCGGAGAGCAACAAACTGTATCT
    TATTTATCAATGGTATTAAATGATATCGGTGTGAATGCCAAAGCAATGACTGGCTATCAA
    GCAGGTATTAAAACCATTGGCCATCATTTAAAAAGTAAAATTGCTCAAATTAATCCTCAA
    ACATTTGAACAAGCCTTTCAAGAAAACGATATTTTAGTAGTTGCTGGATTTCAAGGCATC
    AATGAACATCAGGAATTAACAACTTTAGGCAGAGGCGGTTCTGATACGACCGCTGTGGCA
    CTTGCTGTTAGTAATCAAATACCTTGTGAAATTTATACCGACGTTGATGGTGTGTATGCC
    ACTGACCCAAGACTTTTACCAAAAGCTAAACGACTAGACATCGTCTCATATGAAGAAATG
    ATGGAAATGAGCGCTTTAGGTGCTGGTGTACTTGAAACAAGAAGTGTTGAATTAGCTAAA
    AACTATAATATCCCTTTATATTTAGGAAAAACTTTATCGAACGTGAAAGGAACATGGATT
    ATGTCAAATGAAGAAATATTAGAGAAAAAAGCAGTTACTGGTGTGGCTTTGGATAAACAT
    ATGATGCATGTAACAATTAGTTATCCCCTACCTGACAATCAGCTACTTACCCAACTATTT
    ACGGAACTTGAAGAAGGTGCTGTAAATGTTGATATGATTTCACAAATCGTCAACTTGGAT
    GGGCTACAACTATCCTTCACGATTAAAGATAGTGATTTTCATCAAATTTCTATGATTCTT
    GAAACATTAAAGAATCAATATGAAGCATTAGCTTATAAAATCAATGAGCATTATGTCAAA
    ATTTCATTAATTGGCTCAGGCATGCGTGATATGTCAGGTGTGGCATCAAAAGCATTTTTG
    ACATTAATTGAAAATAATATACCTTTCTACCAAACAACAACATCTGAAATAAGTATTTCA
    TACGTCATTGATGATTTTAATGGGCAACAAGCGGTAGAAAAACTATATGACGCATTTAAC
    ATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1206

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001377
  • symbol: JSNZ_001377
  • description: aspartate kinase
  • length: 401
  • theoretical pI: 4.70763
  • theoretical MW: 44342.5
  • GRAVY: -0.0533666

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.2.4?  ExPASy
    Aspartate kinase ATP + L-aspartate = ADP + 4-phospho-L-aspartate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family aspartate kinase (TIGR00657; EC 2.7.2.4; HMM-score: 354.5)
    Metabolism Amino acid biosynthesis Aspartate family aspartate kinase, monofunctional class (TIGR00656; EC 2.7.2.4; HMM-score: 331.8)
    and 4 more
    Metabolism Amino acid biosynthesis Aspartate family putative Pyrococcus aspartate kinase subunit (TIGR02078; EC 2.7.2.4; HMM-score: 105.3)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions UMP kinase (TIGR02075; EC 2.7.4.22; HMM-score: 36.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions putative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 32.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine pyrophosphokinase (TIGR01378; EC 2.7.6.2; HMM-score: 16.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 155.9)
    and 4 more
    ACT (CL0070) ACT_9; ACT domain (PF22468; HMM-score: 68.7)
    ACT_7; ACT domain (PF13840; HMM-score: 31.6)
    no clan defined TPK_catalytic; Thiamin pyrophosphokinase, catalytic domain (PF04263; HMM-score: 17)
    E-set (CL0159) Alpha_adaptinC2; Adaptin C-terminal domain (PF02883; HMM-score: 16.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9876
    • Cytoplasmic Membrane Score: 0.0085
    • Cell wall & surface Score: 0.0011
    • Extracellular Score: 0.0027
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004219
    • TAT(Tat/SPI): 0.000769
    • LIPO(Sec/SPII): 0.000608
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAMGNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGVNAKAMTGYQAGIKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVALAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVELAKNYNIPLYLGKTLSNVKGTWIMSNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLLTQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKINEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAVEKLYDAFNI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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