PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000944
• pan locus tag^?: SAUPAN003128000
• symbol: fabF
• pan gene symbol^?: fabF
• synonym:
• product: beta-ketoacyl-ACP synthase II

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000944
• symbol: fabF
• product: beta-ketoacyl-ACP synthase II
• replicon: chromosome
• strand: +
• coordinates: 947825..949069
• length: 1245
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  
  ATGAGTCAAAATAAAAGAGTAGTTATTACAGGTATGGGAGCCCTTTCTCCAATCGGTAAT
  GATGTCAAAACAACATGGGAGAATGCTCTAAAAGGCGTAAATGGTATCGATAAAATTACA
  CGTATTGATACTGAACCTTATAGCGTTCACTTAGCAGGAGAACTTAAAAACTTTAATATT
  GAAGATCATATCGACAAAAAAGAAGCGCGTCGTATGGATAGATTTACTCAATATGCAATT
  GTAGCAGCTAGAGAGGCTGTTAAAGATGCGCAATTAGATATCAATGAAAATACTGCAGAT
  CGAATCGGTGTATGGATTGGTTCTGGTATCGGTGGTATGGAAACATTTGAAATTGCACAT
  AAACAATTAATGGATAAAGGCCCAAGACGTGTGAGTCCATTTTTCGTACCAATGTTAATT
  CCTGATATGGCAACTGGGCAAGTATCAATTGACTTAGGTGCAAAAGGACCAAATGGTGCA
  ACAGTTACAGCATGTGCAACAGGTACAAATTCAATCGGAGAAGCATTTAAAATTGTGCAA
  CGCGGTGATGCAGATGCAATGATTACTGGTGGTACAGAAGCACCAATTACTCATATGGCA
  ATTGCTGGTTTCAGTGCAAGTCGAGCGCTTTCTACAAATGATGACATTGAAACAGCATGT
  CGTCCATTCCAAGAAGGTAGAGACGGTTTTGTTATGGGTGAAGGTGCTGGTATTTTAGTA
  ATCGAATCTTTAGAATCAGCACAAGCTCGAGGTGCCAATATTTATGCTGAGATAGTTGGC
  TATGGTACTACAGGTGATGCTTATCATATTACAGCGCCAGCTCCAGAAGGTGAAGGCGGT
  TCTAGAGCAATGCAAGCAGCTATGGATGATGCTGGTATTGAACCTAAAGACGTACAATAC
  TTAAATGCCCATGGTACAAGTACTCCTGTTGGTGACTTAAATGAAGTTAAAGCTATTAAA
  AATACATTTGGTGAAGCAGCTAAACACTTAAAAGTTAGCTCAACAAAATCAATGACTGGT
  CACTTACTTGGTGCAACAGGTGGAATTGAAGCAATCTTCTCAGCGCTTTCAATTAAAGAC
  TCTAAAGTCGCACCGACAATTCATGCGGTAACACCAGACCCAGAATGTGATTTGGATATT
  GTTCCAAATGAAGCGCAAGACCTTGATATTACTTATGCAATGAGTAATAGCTTAGGATTC
  GGTGGACATAACGCAGTATTAGTATTCAAGAAATTTGAAGCATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1245
  

⊟Protein

• 2GQD
  PDB

Protein Data Bank: 2GQD

⊟General

• locus tag: JSNZ_000944
• symbol: FabF
• description: beta-ketoacyl-ACP synthase II
• length: 414
• theoretical pI: 4.82119
• theoretical MW: 43739
• GRAVY: -0.167633

⊟Function

• reaction:
  EC 2.3.1.179?  ExPASy
  Beta-ketoacyl-[acyl-carrier-protein] synthase II (Z)-hexadec-11-enoyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier-protein] + CO₂ + [acyl-carrier-protein]
• TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis beta-ketoacyl-acyl-carrier-protein synthase II (TIGR03150; EC 2.3.1.179; HMM-score: 639.4)
  and 3 more
  polyketide-type polyunsaturated fatty acid synthase PfaA (TIGR02813; HMM-score: 106.6)
  Metabolism Fatty acid and phospholipid metabolism Other acetyl-CoA C-acyltransferase (TIGR01930; EC 2.3.1.16; HMM-score: 23.7)
  3-oxoadipyl-CoA thiolase (TIGR02430; EC 2.3.1.174; HMM-score: 12.7)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  Thiolase (CL0046) ketoacyl-synt; Beta-ketoacyl synthase, N-terminal domain (PF00109; HMM-score: 205.5)
  and 3 more
  Ketoacyl-synt_C; Beta-ketoacyl synthase, C-terminal domain (PF02801; HMM-score: 134.5)
  Thiolase_N; Thiolase, N-terminal domain (PF00108; HMM-score: 16.6)
  Thiolase_C_1; Thiolase C-terminal domain-like (PF22691; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.05
  • Cytoplasmic Membrane Score: 8.78
  • Cellwall Score: 0.08
  • Extracellular Score: 0.09
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9616
  • Cytoplasmic Membrane Score: 0.0168
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0214
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.036712
  • TAT(Tat/SPI): 0.001296
  • LIPO(Sec/SPII): 0.00713
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDTEPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRIGVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGATVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIETACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPEGEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVSSTKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITYAMSNSLGFGGHNAVLVFKKFEA

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications