PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000943
• pan locus tag^?: SAUPAN003127000
• symbol: JSNZ_000943
• pan gene symbol^?: fabH
• synonym:
• product: beta-ketoacyl-ACP synthase III

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000943
• symbol: JSNZ_000943
• product: beta-ketoacyl-ACP synthase III
• replicon: chromosome
• strand: +
• coordinates: 946872..947813
• length: 942
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  
  ATGAACGTGGGTATTAAAGGTTTTGGTGCATATGCACCAGAAAAGATTATTGACAATGCC
  TATTTTGAGCAATTTTTAGATACATCTGATGAATGGATTTCTAAGATGACTGGAATTAAA
  GAAAGACATTGGGCAGATGACGATCAAGATACTTCAGATTTAGCATATGAAGCAAGTGTA
  AAAGCAATCGCTGACGCTGGTATTCAGCCCGAAGATATAGATATGATAATTGTTGCCACA
  GCAACTGGAGATATGCCATTTCCAACTGTCGCAAATATGTTGCAAGAACGTTTAGGGACG
  GGCAAAGTTGCCTCTATGGATCAACTTGCAGCATGTTCTGGATTTATGTATTCAATGATT
  ACAGCTAAACAATATGTTCAATCTGGAGATTATCATAATATTTTAGTTGTCGGTGCAGAT
  AAATTATCTAAAATAACAGATTTAACTGACCGTTCTACTGCAGTTCTATTTGGAGATGGT
  GCAGGTGCGGTTATCATCGGTGAAGTTTCAGAAGGTAGAGGTATTATAAGTTATGAAATG
  GGTTCTGATGGCACTGGTGGTAAACATTTATATTTAGATAAAGATACTGGTAAACTGAAA
  ATGAATGGTCGAGAAGTATTTAAATTTGCTGTTAGAATTATGGGTGATGCATCAACACGT
  GTAGTTGAAAAAGCGAATTTAACATCAGATGATATAGATTTATTTATTCCTCATCAAGCT
  AATATTAGAATTATGGAATCAGCTAGAGAACGCTTAGGTATTTCAAAAGATAAAATGAGT
  GTTTCTGTAAATAAATATGGAAATACTTCAGCTGCGTCAATACCTTTAAGTATCGATCAA
  GAATTAAAAAATGGTAAACTCAAAGATGATGATACAATTGTTCTTGTCGGATTCGGTGGC
  GGCCTAACTTGGGGCGCAATGACAATAAAATGGGGAAAATAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  942
  

⊟Protein

⊟General

• locus tag: JSNZ_000943
• symbol: JSNZ_000943
• description: beta-ketoacyl-ACP synthase III
• length: 313
• theoretical pI: 4.64033
• theoretical MW: 33879.2
• GRAVY: -0.177955

⊟Function

• reaction:
  EC 2.3.1.180?  ExPASy
  Beta-ketoacyl-[acyl-carrier-protein] synthase III Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO₂
• TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] synthase III (TIGR00747; EC 2.3.1.180; HMM-score: 407.7)
  and 5 more
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis putative hydroxymethylglutaryl-CoA synthase (TIGR00748; EC 2.3.3.10; HMM-score: 63.9)
  hydroxymethylglutaryl-CoA synthase (TIGR01835; EC 2.3.3.10; HMM-score: 32.4)
  Metabolism Fatty acid and phospholipid metabolism Other acetyl-CoA C-acyltransferase (TIGR01930; EC 2.3.1.16; HMM-score: 28.6)
  hydroxymethylglutaryl-CoA synthase (TIGR01833; EC 2.3.3.10; HMM-score: 18.2)
  Cellular processes Cellular processes Sporulation and germination stage V sporulation protein AD (TIGR02845; HMM-score: 16.8)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  Thiolase (CL0046) ACP_syn_III_C; 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal (PF08541; HMM-score: 130.7)
  and 10 more
  ACP_syn_III; 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III (PF08545; HMM-score: 102.5)
  Thiolase_N; Thiolase, N-terminal domain (PF00108; HMM-score: 39.2)
  Chal_sti_synt_N; Chalcone and stilbene synthases, N-terminal domain (PF00195; HMM-score: 23.4)
  SpoVAD; Stage V sporulation protein AD (SpoVAD) (PF07451; HMM-score: 20.8)
  HMG_CoA_synt_N; Hydroxymethylglutaryl-coenzyme A synthase N terminal (PF01154; HMM-score: 19.2)
  Chal_sti_synt_C; Chalcone and stilbene synthases, C-terminal domain (PF02797; HMM-score: 19)
  FAE1_CUT1_RppA; FAE1/Type III polyketide synthase-like protein (PF08392; HMM-score: 18.7)
  Thiolase_C_1; Thiolase C-terminal domain-like (PF22691; HMM-score: 17.1)
  Thiolase_C; Thiolase, C-terminal domain (PF02803; HMM-score: 16.2)
  ketoacyl-synt; Beta-ketoacyl synthase, N-terminal domain (PF00109; HMM-score: 13.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helix: 1
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9882
  • Cytoplasmic Membrane Score: 0.0024
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0093
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002562
  • TAT(Tat/SPI): 0.000195
  • LIPO(Sec/SPII): 0.000304
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHWADDDQDTSDLAYEASVKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGKVASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDGAGAVIIGEVSEGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDASTRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPLSIDQELKNGKLKDDDTIVLVGFGGGLTWGAMTIKWGK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications