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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000509
  • pan locus tag?: SAUPAN002323000
  • symbol: hchA
  • pan gene symbol?: hchA
  • synonym:
  • product: glyoxalase III HchA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000509
  • symbol: hchA
  • product: glyoxalase III HchA
  • replicon: chromosome
  • strand: +
  • coordinates: 543094..543972
  • length: 879
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTCACAAGATGTAAATGAATTAAGTAAGCAACCAACGCCAGATAAAGCAGAAGATAAT
    GCATTTTTCCCATCACCATATTCCCTTAGTCAATATACAGCACCTAAAACAGATTTTGAT
    GGTGTTGAACACAAAGGTGCCTATAAAGATGGTAAATGGAAAGTATTGATGATTGCTGCT
    GAAGAGAGATATGTATTATTGGAAAATGGAAAAATGTTCTCTACGGGTAATCATCCTGTT
    GAAATGTTATTACCTTTACATCATTTAATGGAAGCAGGTTTTGACGTTGATGTTGCGACA
    TTATCTGGTTATCCAGCTAAATTAGAATTATGGGCTATGCCAACTGAAGACGAGGCAGTT
    ATAAGCACTTATAATAAATTGAAAGAAAAATTAAAACAGCCTAAAAAATTAGCAGATGTG
    ATTAAAAATGAATTAGGACCGGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACAT
    GCTGCAGTTGTTGGTATTTCTGAAAGTGAGGACGTTCAACAAACATTAGATTGGGCATTA
    GACAATGACCGCTTTATAGTTACATTATGTCATGGACCAGCAGCACTACTTTCAGCAGGG
    CTTAACAGAGAAAAATCTCCATTAGAAGGATACTCTGTTTGTGTCTTCCCTGACTCATTA
    GATGAAGGTGCAAATATTGAAATAGGTTATTTACCTGGACGCTTGAAATGGTTAGTTGCT
    GATTTATTAACTAAACAAGGATTAAAAGTAGTTAACGACGATATGACAGGAAGAACGTTA
    AAAGATCGTAAATTATTAACAGGTGACAGTCCTTTAGCTTCAAATGAGTTAGGAAAATTA
    GCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    879

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000509
  • symbol: HchA
  • description: glyoxalase III HchA
  • length: 292
  • theoretical pI: 4.65565
  • theoretical MW: 32148.4
  • GRAVY: -0.316096

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.1.124?  ExPASy
    Protein deglycase An N(omega)-(1-hydroxy-2-oxopropyl)-[protein]-L-arginine + H2O = a [protein]-L-arginine + (R)-lactate An N6-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H2O = a [protein]-L-lysine + (R)-lactate An S-(1-hydroxy-2-oxopropyl)-[protein]-L-cysteine + H2O = a [protein]-L-cysteine + (R)-lactate
  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides intracellular protease, PfpI family (TIGR01382; HMM-score: 28.5)
    and 1 more
    Unknown function General DJ-1 family protein (TIGR01383; HMM-score: 17.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Glutaminase_I (CL0014) DJ-1_PfpI; DJ-1/PfpI family (PF01965; HMM-score: 39.8)
    and 3 more
    ThiJ_like; ThiJ/PfpI family-like (PF17124; HMM-score: 19.5)
    no clan defined DUF1657; Protein of unknown function (DUF1657) (PF07870; HMM-score: 12.2)
    RND_permease (CL0322) ACR_tran; AcrB/AcrD/AcrF family (PF00873; HMM-score: 9.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.931
    • Cytoplasmic Membrane Score: 0.0032
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0658
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006928
    • TAT(Tat/SPI): 0.001034
    • LIPO(Sec/SPII): 0.001259
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEHKGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLSGYPAKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGHAAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPDSLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNELGKLAVNEMLNAIQNK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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