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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_0513 [new locus tag: NWMN_RS02980 ]
- pan locus tag?: SAUPAN002323000
- symbol: NWMN_0513
- pan gene symbol?: hchA
- synonym:
- product: chaperone protein HchA
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_0513 [new locus tag: NWMN_RS02980 ]
- symbol: NWMN_0513
- product: chaperone protein HchA
- replicon: chromosome
- strand: +
- coordinates: 591512..592390
- length: 879
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5330268 NCBI
- RefSeq: YP_001331547 NCBI
- BioCyc:
- MicrobesOnline: 3706060 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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841ATGTCACAAGATGTAAATGAATTAAGTAAGCAACCAACGCCAGATAAAGCAGAAGATAAC
GCATTTTTCCCATCACCATATTCCCTTAGTCAATATACAGCACCTAAAACAGATTTTGAT
GGTGTTGAACACAAAGGTGCCTATAAAGATGGTAAATGGAAAGTATTGATGATTGCTGCT
GAAGAGCGATATGTATTATTGGAAAATGGAAAAATGTTCTCTACGGGTAATCATCCTGTT
GAAATGTTATTACCTTTACATCATTTAATGGAAGCAGGTTTTGACGTTGATGTTGCGACA
TTATCTGGTTATCCAGTTAAATTAGAATTATGGGCTATGCCAACTGAAGACGAGGCAGTT
ATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCCAAAAAAATTAGCAGATGTG
ATTAAAAATGAATTAGGACCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACAT
GCTGCAGTTGTTGGTATTTCTGAAAGTGAGGACGTTCAACAAACATTAGATTGGGCATTA
GACAATGACCGCTTTATAGTTACATTATGTCATGGACCAGCAGCACTACTTTCAGCAGGG
ATTAACAGAGAAAAATCTCCATTAGAAGGATACTCTGTTTGTGTCTTCCCTGACTCATTA
GATGAAGGTGCAAATATTGAAATAGGTTATTTACCTGGACGCTTGAAATGGTTAGTTGCT
GATTTATTAACTAAACAAGGATTAAAAGTAGTTAACGACGATATGACAGGAAGAACGTTA
AAAGATCGTAAATTATTAACAGGTGACAGTCCTTTAGCTTCAAATGAGTTAGGAAAATTA
GCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_0513 [new locus tag: NWMN_RS02980 ]
- symbol: NWMN_0513
- description: chaperone protein HchA
- length: 292
- theoretical pI: 4.65565
- theoretical MW: 32176.4
- GRAVY: -0.305479
⊟Function[edit | edit source]
- reaction: EC 3.5.1.124? ExPASyProtein deglycase An N(omega)-(1-hydroxy-2-oxopropyl)-[protein]-L-arginine + H2O = a [protein]-L-arginine + (R)-lactate An N6-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H2O = a [protein]-L-lysine + (R)-lactate An S-(1-hydroxy-2-oxopropyl)-[protein]-L-cysteine + H2O = a [protein]-L-cysteine + (R)-lactateEC 3.1.2.-? ExPASy
- TIGRFAM: Protein fate Degradation of proteins, peptides, and glycopeptides intracellular protease, PfpI family (TIGR01382; HMM-score: 30.5)and 1 moreUnknown function General DJ-1 family protein (TIGR01383; HMM-score: 18.3)
- TheSEED :
- Chaperone protein hchA
- PFAM: Glutaminase_I (CL0014) DJ-1_PfpI; DJ-1/PfpI family (PF01965; HMM-score: 40.8)and 2 moreThiJ_like; ThiJ/PfpI family-like (PF17124; HMM-score: 18.5)RND_permease (CL0322) ACR_tran; AcrB/AcrD/AcrF family (PF00873; HMM-score: 10)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
- DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9357
- Cytoplasmic Membrane Score: 0.003
- Cell wall & surface Score: 0
- Extracellular Score: 0.0613
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.006928
- TAT(Tat/SPI): 0.001034
- LIPO(Sec/SPII): 0.001259
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEHKGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLSGYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGHAAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGINREKSPLEGYSVCVFPDSLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNELGKLAVNEMLNAIQNK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator: SigB (activation) regulon
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
J Bacteriol: 2004, 186(13);4085-99
[PubMed:15205410] [WorldCat.org] [DOI] (P p) - ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
J Bacteriol: 2011, 193(18);4954-62
[PubMed:21725011] [WorldCat.org] [DOI] (I p)