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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000270
  • pan locus tag?: SAUPAN001240000
  • symbol: JSNZ_000270
  • pan gene symbol?: nanK
  • synonym:
  • product: ROK family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000270
  • symbol: JSNZ_000270
  • product: ROK family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 305688..306548
  • length: 861
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCGGTTATTGATAAG
    CAATTGAATATGTTTGACTATCAACAAATACCAACGCCGGACAACAAAAGTGAGCTTATT
    ACTGACAAAGTATATGAGATTGTAACAGGATATATGAAGCAATATCAGTTGATCCAACCT
    GTCATAGGTATTTCATCAGCAGGCGTTGTTGATGAACAAAAAGGCGAAATTGTATACGCA
    GGGCCAACCATTCCGAATTATAAAGGTACTAATTTTAAGCGATTATTAAAATCACTGTCT
    CCTTATGTCAAAGTAAAAAATGATGTAAACGCTGCATTACTAGGCGAATTGAAATTACAT
    CAATATCAAGCAGAACGGATCTTTTGTATGACGCTTGGTACAGGCATTGGGGGTGCGTAC
    AAAAATAATCAAGGTCATATTGATAATGGTGAACTTCATAAGGCAAATGAAGTTGGGTAT
    TTATTGTATCGTCCAACTGAAAATACAACGTTTGAGCAACGTGCTGCAACGAGTGCATTG
    AAAAAGCGCATGATTGCCGGAGGATTTACGAGAAGCACACATGTGCCAGTATTGTTTGAA
    GCAGCTGAAGAAGGTGATGATATTGCAAAACAAATATTGAATGAGTGGGCAGAAGATGTA
    GCAGAAGGGATTGCCCAAATACAAGTCATGTATGATCCAGGGCTTATATTAATTGGTGGC
    GGTATATCTGAACAAGGAGACAATCTCATTAAATATATCGAGCCGAAAGTTGCACACTAT
    TTACCAAAAGACTATGTTTATGCACCAATACAAACGACTAAGAGTAAAAATGATGCAGCA
    TTATATGGCTGTTTGCAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
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    660
    720
    780
    840
    861

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000270
  • symbol: JSNZ_000270
  • description: ROK family protein
  • length: 286
  • theoretical pI: 5.69776
  • theoretical MW: 31693
  • GRAVY: -0.265035

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function General ROK family protein (putative glucokinase) (TIGR00744; HMM-score: 114.7)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Actin_ATPase (CL0108) ROK; ROK family (PF00480; HMM-score: 123.8)
    and 3 more
    BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 22.2)
    Pan_kinase; Type III pantothenate kinase (PF03309; HMM-score: 14.9)
    FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 14.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9979
    • Cytoplasmic Membrane Score: 0.001
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0009
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004667
    • TAT(Tat/SPI): 0.000115
    • LIPO(Sec/SPII): 0.000646
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MYYIAIDIGGTQIKSAVIDKQLNMFDYQQIPTPDNKSELITDKVYEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPYVKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGYLLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAEDVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSKNDAALYGCLQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: NanR* (repression) regulon, CcpA regulon
    NanR*(TF)important in Sialic acid utilization, N-acetylglucosamine utilization;  regulation predicted or transferred from N315 and NCTC 8325  [1]
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Michael E Olson, Jessica M King, Timothy L Yahr, Alexander R Horswill
Sialic acid catabolism in Staphylococcus aureus.
J Bacteriol: 2013, 195(8);1779-88
[PubMed:23396916] [WorldCat.org] [DOI] (I p)

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