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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000124
  • pan locus tag?: SAUPAN001021000
  • symbol: argB
  • pan gene symbol?: argB
  • synonym:
  • product: acetylglutamate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000124
  • symbol: argB
  • product: acetylglutamate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 147513..148277
  • length: 765
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAATTTATTGTCATTAAAATTGGTGGCAGTACACTTAGTGACATGCATCCATCAATT
    ATTAACAATATTAAGCATTTACGATTAAACAACATCTACCCTATTATCGTTCATGGCGGT
    GGCCCATTTATTAATGATGCATTATCAAACCAGCAAATCGAGCCACACTTTGTTAATGGC
    CTAAGAGTGACTGATAAAGCAACCATGACCATTACTAAACACACGCTCATTGCAGACGTT
    AACACTGCATTAGTAGCTCAATTTAACCAGCACCAATGTTCTGCGATAGGCTTATGTGGT
    TTGGATGCTCAATTATTTGAAATTAAACGTTTTGATCAACAATATGGATATGTCGGTGTC
    CCGACCGCTTTAAATAAGGATGCTTTACAGTATATATGTACTAAATTTGTACCTATCATC
    AATTCGATTGGTTTCAATAACCATGATGGAGAATTTTACAATATTAATGCTGACACGCTT
    GCCTATTTTATTGCATCATCATTAAAAGCGCCTATTTATGTATTAAGTAATATTGCAGGT
    GTACTCATCAATGATGTGGTTATACCTCAATTGCCATTAGTCGATATTCATCAATATATT
    GAACATGGTGATATTTATGGAGGTATGATTCCCAAAGTGCTAGATGCCAAAAATGCGATT
    GAAAATGGCTGTCCTAAAGTTATCATTGCATCAGGAAACAAGCCAAATATCATTGAATCT
    ATTTACAATAATGATTTTGTTGGCACAACAATCCTTAATTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    765

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000124
  • symbol: ArgB
  • description: acetylglutamate kinase
  • length: 254
  • theoretical pI: 6.59407
  • theoretical MW: 27846.9
  • GRAVY: 0.177559

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.2.8?  ExPASy
    Acetylglutamate kinase ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family acetylglutamate kinase (TIGR00761; EC 2.7.2.8; HMM-score: 278.9)
    and 3 more
    Metabolism Amino acid biosynthesis Glutamate family amino-acid N-acetyltransferase (TIGR01890; EC 2.3.1.1; HMM-score: 47.5)
    Metabolism Amino acid biosynthesis Glutamate family glutamate 5-kinase (TIGR01027; EC 2.7.2.11; HMM-score: 18.5)
    Metabolism Amino acid biosynthesis Glutamate family delta l-pyrroline-5-carboxylate synthetase (TIGR01092; HMM-score: 16.3)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 150)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9919
    • Cytoplasmic Membrane Score: 0.0027
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.005
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007049
    • TAT(Tat/SPI): 0.000145
    • LIPO(Sec/SPII): 0.001216
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKFIVIKIGGSTLSDMHPSIINNIKHLRLNNIYPIIVHGGGPFINDALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLDAQLFEIKRFDQQYGYVGVPTALNKDALQYICTKFVPIINSIGFNNHDGEFYNINADTLAYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKNAIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: ArgR/AhrC (repression) regulon
    ArgR/AhrC(TF)important in Arginine biosynthesis, Arginine degradation;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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