PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000053
• pan locus tag^?: SAUPAN000909000
• symbol: spa
• pan gene symbol^?: spa
• synonym:
• product: staphylococcal protein A

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000053
• symbol: spa
• product: staphylococcal protein A
• replicon: chromosome
• strand: -
• coordinates: 63640..65166
• length: 1527
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  
  TTGAAAAAGAAAAACATTTATTCAATTCGTAAACTAGGTGTAGGTATTGCATCTGTAACT
  TTAGGTACATTACTTATATCTGGTGGCGTAACACCTGCTGCAAATGCTGCGCAACACGAT
  GAAGCTCAACAAAATGCTTTTTATCAAGTGTTAAATATGCCTAACTTAAACGCTGATCAA
  CGTAATGGTTTTATCCAAAGCCTTAAAGACGATCCAAGCCAAAGTGCTAACGTTTTAGGT
  GAAGCTCAAAAACTTAATGACTCTCAAGCTCCAAAAGCTGATGCGCAACAAAATAACTTC
  AACAAAGATCAACAAAGCGCCTTCTATGAAATCTTGAACATGCCTAACTTAAACGAAGCG
  CAACGCAATGGTTTCATTCAAAGTCTTAAAGACGACCCAAGCCAAAGCACTAATGTTTTA
  GGTGAAGCTAAAAAATTAAACGAATCTCAAGCACCGAAAGCGGATAACAAATTCAACAAA
  GAACAACAAAATGCTTTCTATGAAATCTTGAACATGCCTAACTTGAACGAAGAACAACGC
  AATGGTTTCATCCAAAGCTTAAAAGATGACCCAAGTCAAAGTGCTAACCTATTGTCAGAA
  GCTAAAAAGTTAAATGAATCTCAAGCACCGAAAGCGGATAACAAATTCAACAAAGAACAA
  CAAAATGCTTTCTATGAAATCTTACATTTACCTAACTTAAACGAAGAACAACGCAATGGT
  TTCATCCAAAGCTTAAAAGATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAA
  AAGCTAAATGATGCACAAGCACCAAAAGCTGACAACAAATTCAACAAAGAACAACAAAAT
  GCTTTCTATGAAATTTTACATTTACCTAACTTAACTGAAGAACAACGTAACGGCTTCATC
  CAAAGCCTTAAAGACGATCCTTCAGTGAGCAAAGAAATTTTAGCAGAAGCTAAAAAGCTA
  AACGATGCTCAAGCACCAAAAGAGGAAGACAACAACAAACCTGGTAAAGAAGACAACAAC
  AAGCCTGGTAAAGAAGACAACAACAAGCCTGGCAAAGAAGACGGCAACAAGCCTGGTAAA
  GAAGACAACAACAAACCTGGTAAAGAAGACAACAAAAAACCTGGTAAAGAAGACAACAAA
  AAACCTGGTAAAGAAGACAACAAAAAACCTGGTAAAGAAGATGGCAACAAGCCTGGCAAA
  GAAGACAACAAAAAACCTGGTAAAGAAGACGGCAACGGAGTACATGTCGTTAAACCTGGT
  GATACAGTAAATGACATTGCAAAAGCAAACGGCACTACTGCTGACAAAATTGCTGCAGAT
  AACAAATTAGCTGATAAAAACATGATCAAACCTGGTCAAGAACTTGTTGTTGATAAGAAG
  CAACCAGCAAACCATGCAGATGCTAACAAAGCTCAAGCATTACCAGAAACTGGTGAAGAA
  AATCCATTCATTGGTACAACTGTATTTGGTGGATTATCATTAGCGTTAGGTGCAGCGTTA
  TTAGCTGGACGTCGTCGCGAACTATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1527
  

⊟Protein

⊟General

• locus tag: JSNZ_000053
• symbol: Spa
• description: staphylococcal protein A
• length: 508
• theoretical pI: 5.89955
• theoretical MW: 55710.2
• GRAVY: -1.14902

⊟Function

• TIGRFAM:
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 34.6)
  and 3 more
  Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 19.4)
  Cellular processes Cellular processes Sporulation and germination spore coat assembly protein SafA (TIGR02899; HMM-score: 17.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs bacillithiol biosynthesis deacetylase BshB2 (TIGR04000; EC 3.-.-.-; HMM-score: 8.3)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  B_GA (CL0598) B; B domain (PF02216; HMM-score: 474.4)
  and 21 more
  GA; GA module (PF01468; HMM-score: 82.1)
  LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 50.2)
  no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 39.8)
  UFL1; E3 UFM1-protein ligase 1-like domain (PF23659; HMM-score: 32)
  Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 31.8)
  Octapeptide; Octapeptide repeat (PF03373; HMM-score: 26.6)
  VBS-like (CL0705) Talin_IBS2B; Talin IBS2B domain (PF21896; HMM-score: 22.4)
  Acetyltrans (CL0257) HAT1_C; Histone acetyltransferase type B catalytic subunit, C-terminal (PF21183; HMM-score: 22.1)
  no clan defined MRP-63; Mitochondrial ribosome protein 63 (PF14978; HMM-score: 17)
  IFT43; Intraflagellar transport protein 43 (PF15305; HMM-score: 17)
  Dim_A_B_barrel (CL0032) DUF3291; Domain of unknown function (DUF3291) (PF11695; HMM-score: 16.8)
  TerB (CL0414) TerB; Tellurite resistance protein TerB (PF05099; HMM-score: 16.4)
  SNARE-fusion (CL0445) V-SNARE; Vesicle transport v-SNARE protein N-terminus (PF05008; HMM-score: 14.8)
  no clan defined CLAMP; Flagellar C1a complex subunit C1a-32 (PF14769; HMM-score: 14.8)
  LysM (CL0187) LysM3_LYK4_5; LYK4/5 LysM3 domain (PF23473; HMM-score: 14.4)
  E-set (CL0159) GH85_C; GH85 endohexosaminidases, C-terminal domain (PF21910; HMM-score: 13.8)
  post-AAA (CL0604) DNApol3-delta_C; DNA polymerase III, delta subunit, C terminal (PF09115; HMM-score: 10.2)
  no clan defined NTS_2; N-terminal segments of P. falciparum erythrocyte membrane protein (PF15448; HMM-score: 9.7)
  MMgT; Membrane magnesium transporter (PF10270; HMM-score: 9.1)
  ANIS5_cation-bd; SXP/RAL-2 family protein Ani s 5-like, metal-binding domain (PF02520; HMM-score: 8.9)
  Met_repress (CL0057) DUF1778; Protein of unknown function (DUF1778) (PF08681; HMM-score: 6.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 9.98
  • Extracellular Score: 0.01
  • Internal Helices: 2
• DeepLocPro: Cell wall & surface
  • Cytoplasmic Score: 0.0001
  • Cytoplasmic Membrane Score: 0.0371
  • Cell wall & surface Score: 0.9138
  • Extracellular Score: 0.0489
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 36 aa
  • SP(Sec/SPI): 0.986537
  • TAT(Tat/SPI): 0.008981
  • LIPO(Sec/SPII): 0.003804
  • Cleavage Site: CS pos: 36-37. ANA-AQ. Pr: 0.9494
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNKFNKEQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNKEQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNKEQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKPGKEDNNKPGKEDNNKPGKEDGNKPGKEDNNKPGKEDNKKPGKEDNKKPGKEDNKKPGKEDGNKPGKEDNKKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIKPGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Andrea C DeDent, Molly McAdow, Olaf Schneewind
Distribution of protein A on the surface of Staphylococcus aureus.
J Bacteriol: 2007, 189(12);4473-84
[PubMed:17416657] [WorldCat.org] [DOI] (P p)Hwan Keun Kim, Vilasack Thammavongsa, Olaf Schneewind, Dominique Missiakas
Recurrent infections and immune evasion strategies of Staphylococcus aureus.
Curr Opin Microbiol: 2012, 15(1);92-9
[PubMed:22088393] [WorldCat.org] [DOI] (I p)Samuel Becker, Matthew B Frankel, Olaf Schneewind, Dominique Missiakas
Release of protein A from the cell wall of Staphylococcus aureus.
Proc Natl Acad Sci U S A: 2014, 111(4);1574-9
[PubMed:24434550] [WorldCat.org] [DOI] (I p)