⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA_RS06050 [old locus tag: SA1067 ]
pan locus tag^?: SAUPAN003507000
symbol: SA_RS06050
pan gene symbol^?: rpmB
• synonym:
• product: 50S ribosomal protein L28

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS06050 [old locus tag: SA1067 ]
• symbol: SA_RS06050
• product: 50S ribosomal protein L28
• replicon: chromosome
• strand: -
• coordinates: 1207155..1207343
• length: 189
• essential: yes DEG other strains

⊟Accession numbers

• Location: NC_002745 (1207155..1207343) NCBI
• BioCyc: SA_RS06050 BioCyc
• MicrobesOnline: see SA1067

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  
  ATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTTCGACTGGTAACAGACGTTCACAC
  GCTTTAAACTCTACTAAACGTAGATGGAACGCTAACCTTCAAAAAGTTAGAATCCTAGTT
  GACGGTAAACCTAAAAAAGTTTGGGTTTCTGCACGTGCTTTAAAATCTGGTAAAGTAACT
  AGAGTTTAA
  60
  120
  180
  189
  

⊟Protein

• 4WCE
  PDB
• 4WFA
  PDB
• 4WFB
  PDB
• 5HKV
  PDB
• 5HL7
  PDB
• 5LI0
  PDB
• 5ND8
  PDB
• 5ND9
  PDB
• 5TCU
  PDB

Protein Data Bank: 4WFA

Protein Data Bank: 4WFB

Protein Data Bank: 5HKV

Protein Data Bank: 5HL7

Protein Data Bank: 5LI0

Protein Data Bank: 5ND8

Protein Data Bank: 5ND9

Protein Data Bank: 5TCU

⊟General

• locus tag: SA_RS06050 [old locus tag: SA1067 ]
• symbol: SA_RS06050
• description: 50S ribosomal protein L28
• length: 62
• theoretical pI: 12.7454
• theoretical MW: 6977.18
• GRAVY: -0.737097

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein bL28 (TIGR00009; HMM-score: 73.4)
• TheSEED: see SA1067
• ⊞⊞PFAM:
  no clan defined Ribosomal_L28; Ribosomal L28 family (PF00830; HMM-score: 68)
  and 1 more
  Ubiquitin (CL0072) DUF348; G5-linked-Ubiquitin-like domain (PF03990; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 10
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Extracellular
  • Cytoplasmic Score: 0.2164
  • Cytoplasmic Membrane Score: 0
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.7836
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.053732
  • TAT(Tat/SPI): 0.026326
  • LIPO(Sec/SPII): 0.016121
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446440053 NCBI
• RefSeq: WP_000517908 NCBI
• UniProt: see SA1067

⊟Protein sequence

• MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKPKKVWVSARALKSGKVTRV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications