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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

organism: Staphylococcus aureus N315

locus tag: SA_RS01520 [old locus tag: SA0258 ]

pan locus tag^?: SAUPAN001154000

symbol: SA_RS01520

pan gene symbol^?: rbsK

synonym:
product: ribokinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SA_RS01520 [old locus tag: SA0258 ]
symbol: SA_RS01520
product: ribokinase
replicon: chromosome
strand: -
coordinates: 312952..313866
length: 915
essential: no DEG other strains

⊟Accession numbers[edit | edit source]

Location: NC_002745 (312952..313866) NCBI
BioCyc: SA_RS01520 BioCyc
MicrobesOnline: see SA0258

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
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ATGACCAACAAAGTTGTTATTTTAGGTTCAACGAATGTCGATCAATTTTTAACAGTTGAA
AGATATGCACAACCAGGCGAAACATTACATGTTGAAGAAGCACAAAAAGCATTCGGCGGA
GGTAAAGGTGCCAACCAGGCTATTGCCACTGCACGCATGCAAGCAGACACAACATTTATT
ACTAAAATTGGCACTGATGGCGTTGCTGATTTCATCTTAGAAGATTTTAAAGCGGCTCAT
ATTGATACATCATATATTATCAAAACAACTGAAGCAAAAACGGGCCAAGCCTTTATCACT
GTGAATGCAGAAGGACAAAACACCATCTATGTTTATGGTGGTGCGAATATGACGATGACA
CCTGAAGATGTTATTAACGCAAAAGACGCTATAATCAATGCAGACTTTGTCGTTGCACAA
TTAGAAGTACCCATCCCGGCTATTATATCTGCATTTGAAATTGCCAAGGCACATGGTGTG
ACGACAGTATTAAATCCTGCACCAGCGAAAGCATTACCTAATGAATTATTATCATTAATC
GATATTATTGTGCCAAACGAAACAGAAGCCGAATTGTTATCTGGGATTAAAGTAACTAAT
GAACAATCTATGAAAGACAATGCCAATTACTTTTTATCTTTAGGCATTAAGACTGTTTTG
ATTACGCTAGGTAAGCAAGGTACATATTTTGCTACTAAAAATCAAAGCCAACACATCGAA
GCTTATAAAGTAAATGCGATTGATACAACTGCTGCAGGCGACACATTTATTGGTGCATTT
GTCAGTCGCTTAAACAAGTCGCAAGATAACTTAGCAGATGCTATTGATTTTGGTAATAAA
GCGAGCTCACTCACTGTACAAAAACACGGCGCGCAAGCATCTATTCCTCTACTAGAAGAA
GTAAATCAAGTTTAA
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915

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SA_RS01520 [old locus tag: SA0258 ]
symbol: SA_RS01520
description: ribokinase
length: 304
theoretical pI: 4.6011
theoretical MW: 32450.5
GRAVY: 0.0101974

⊟Function[edit | edit source]

⊞reaction:
EC 2.7.1.15? ExPASy
Ribokinase ATP + D-ribose = ADP + D-ribose 5-phosphate
⊞TIGRFAM:
Metabolism Energy metabolism Sugars ribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 382.2)
and 7 more
Metabolism Energy metabolism Sugars 5-dehydro-2-deoxygluconokinase (TIGR04382; EC 2.7.1.92; HMM-score: 116.5)
hexose kinase, 1-phosphofructokinase family (TIGR03168; EC 2.7.1.-; HMM-score: 93.7)
1-phosphofructokinase (TIGR03828; EC 2.7.1.56; HMM-score: 86.1)
Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides bifunctional protein RfaE, domain I (TIGR02198; EC 2.7.1.-; HMM-score: 83.5)
Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (TIGR00097; EC 2.7.1.49,2.7.4.7; HMM-score: 52.2)
Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides tagatose-6-phosphate kinase (TIGR01231; EC 2.7.1.144; HMM-score: 26.2)
Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 25)
TheSEED: see SA0258
⊞PFAM:
Ribokinase (CL0118) PfkB; pfkB family carbohydrate kinase (PF00294; HMM-score: 230.5)
and 3 more
Phos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 52)
NADP_Rossmann (CL0063) Glyco_hydro_4; Family 4 glycosyl hydrolase (PF02056; HMM-score: 14)
E-set (CL0159) Adeno_E3_CR1; Adenovirus E3 region protein CR1 (PF02440; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors: Mg²⁺
effectors:

⊟Localization[edit | edit source]

⊞
PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
⊞
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9975
- Cytoplasmic Membrane Score: 0.0002
- Cell wall & surface Score: 0.0003
- Extracellular Score: 0.002
LocateP:
⊞
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.014499
- TAT(Tat/SPI): 0.001126
- LIPO(Sec/SPII): 0.001169
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 446104891 NCBI
RefSeq: WP_000182746 NCBI
UniProt: see SA0258

⊟Protein sequence[edit | edit source]

MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGANQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKTTEAKTGQAFITVNAEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGVTTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKTVLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAIDFGNKASSLTVQKHGAQASIPLLEEVNQV

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

regulator: CcpA, RbsR* see SA0258

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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