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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

organism: Staphylococcus aureus COL

locus tag: SACOL_RS07830 [old locus tag: SACOL1536 ]

pan locus tag^?: SAUPAN004026000

symbol: SACOL_RS07830

pan gene symbol^?: rluB

synonym:
product: rRNA pseudouridine synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SACOL_RS07830 [old locus tag: SACOL1536 ]
symbol: SACOL_RS07830
product: rRNA pseudouridine synthase
replicon: chromosome
strand: -
coordinates: 1574943..1575680
length: 738
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Location: NC_002951 (1574943..1575680) NCBI
BioCyc: SACOL_RS07830 BioCyc
MicrobesOnline: see SACOL1536

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
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361
421
481
541
601
661
721
ATGACTAAAGAATTAGAACGATTACAAAAACGTATAGCTAATAGCGGTTATACTTCAAGA
CGTAAGGCAGAAACTTTAATTTCTGAAGGAAAAGTGAAAGTGAATGGTACGACTGTCACT
GAATTAGGAACAAAAGTGAAGCCATCAGATACGATTGAAGTTGAAGGTATTAAAATAGAA
CTAGAAGACAAAATTTACATTTTATTCCATAAACCAACTCAAGTGATAACAAGTGTATCT
GATGATAGAGGACGTACAGTGGTTACAGATTACTTTAAAGATATAGAAGCACGTATTTAT
CCTGTTGGTCGTTTAGACTATGATACTTCTGGATTGTTATTACTCACAAATGATGGTGAA
TTTACTAATTTAATGACACATCCAAGATATCAAATTAAGAAAAAATATGTTGCGAAATTA
AAAGGTTATTTAATGAGAGAAGAAGTGAAAGCGCTAGAAAAAGGTATTGAATTAGAAGAT
GGTATGACGCAACCGGCTGAAGTGAAAGTTAAGAAACAAGATAAAGATAAAAACACAACA
TTGGTTGAAATTACAATTACAGAAGGTCGTAATCGCCAAGTCAGAAGAATGTTCGAACAT
TTTGGTCATCAAGTGACGAAACTGTCTCGTATTGAATATGGGCCTTTAAATGTTGTCGGT
TTGAATGCTGGTGAAGGACGTGTATTGACGCCTCATGAAGTAAAAGTGATGCGTCATTTA
GCAGAACATGGGAAATAA
60
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240
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420
480
540
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720
738

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SACOL_RS07830 [old locus tag: SACOL1536 ]
symbol: SACOL_RS07830
description: rRNA pseudouridine synthase
length: 245
theoretical pI: 9.8648
theoretical MW: 27969
GRAVY: -0.606122

⊟Function[edit | edit source]

⊞reaction:
EC 4.2.1.70? ExPASy
Pseudouridylate synthase Uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H₂O
EC 5.4.99.-? ExPASy
⊞TIGRFAM:
Genetic information processing Protein synthesis tRNA and rRNA base modification pseudouridine synthase (TIGR00093; EC 5.4.99.-; HMM-score: 167)
and 6 more
Genetic information processing Protein synthesis tRNA and rRNA base modification pseudouridine synthase, RluA family (TIGR00005; EC 5.4.99.-; HMM-score: 60.7)
Genetic information processing DNA metabolism DNA replication, recombination, and repair S4 domain protein YaaA (TIGR02988; HMM-score: 20.7)
Metabolism Energy metabolism Photosynthesis photosystem II S4 domain protein (TIGR03069; HMM-score: 19.1)
Unknown function General TlyA family rRNA methyltransferase/putative hemolysin (TIGR00478; HMM-score: 17.4)
Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS4 (TIGR01017; HMM-score: 17.1)
Genetic information processing Protein synthesis tRNA and rRNA base modification pseudouridine synthase Rlu family protein, TIGR01621 (TIGR01621; EC 5.4.99.-; HMM-score: 16.3)
TheSEED: see SACOL1536
⊞PFAM:
PseudoU_synth (CL0649) PseudoU_synth_2; RNA pseudouridylate synthase (PF00849; HMM-score: 95)
and 6 more
S4 (CL0492) S4; S4 domain (PF01479; HMM-score: 50.1)
S4_2; S4 domain (PF13275; HMM-score: 24.9)
SYY_C-terminal; Tyrosine--tRNA ligase SYY-like C-terminal domain (PF22421; HMM-score: 24.6)
tRNA_bind_arm (CL0298) Val_tRNA-synt_C; Valyl tRNA synthetase tRNA binding arm (PF10458; HMM-score: 14.7)
TPR (CL0020) PPR_long; Pentacotripeptide-repeat region of PRORP (PF17177; HMM-score: 14.1)
no clan defined Ntox47; Bacterial toxin 47 (PF15540; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

⊞
PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
⊞
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9939
- Cytoplasmic Membrane Score: 0.0038
- Cell wall & surface Score: 0.0006
- Extracellular Score: 0.0017
LocateP:
⊞
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.010895
- TAT(Tat/SPI): 0.001817
- LIPO(Sec/SPII): 0.002516
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 446081722 NCBI
RefSeq: WP_000159577 NCBI
UniProt: see SACOL1536

⊟Protein sequence[edit | edit source]

MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGTKVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPVGRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELEDGMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNVVGLNAGEGRVLTPHEVKVMRHLAEHGK

⊟Experimental data[edit | edit source]

experimentally validated: see SACOL1536
protein localization: see SACOL1536
quantitative data / protein copy number per cell: see SACOL1536
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

regulator:

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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