PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1324 [new locus tag: SA_RS07510 ]
• pan locus tag^?: SAUPAN004026000
• symbol: rluB
• pan gene symbol^?: rluB
• synonym:
• product: ribosomal large subunit pseudouridine synthase B

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1324 [new locus tag: SA_RS07510 ]
• symbol: rluB
• product: ribosomal large subunit pseudouridine synthase B
• replicon: chromosome
• strand: -
• coordinates: 1533429..1534166
• length: 738
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124164 NCBI
• RefSeq: NP_374607 NCBI
• BioCyc: see SA_RS07510
• MicrobesOnline: 103633 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACTAAAGAATTAGAACGATTACAAAAACGTATAGCTAATAGCGGTTATACTTCAAGA
  CGTAAGGCAGAAACTTTAATTTCTGAAGGAAAAGTGAAAGTGAATGGTACGACTGTCACT
  GAATTAGGAACAAAAGTGAAGCCATCAGATACGATTGAAGTTGAAGGTATTAAAATAGAA
  CTAGAAGACAAAATTTACATTTTATTCCATAAACCAACTCAAGTGATAACAAGTGTATCT
  GATGATAGAGGACGTACAGTGGTTACAGATTACTTTAAAGATATAGAAGCACGTATTTAT
  CCTGTTGGTCGTTTAGACTATGATACTTCTGGATTGTTATTACTTACAAATGATGGTGAA
  TTTACTAATTTAATGACACATCCAAGATATCAAATTAAGAAGAAATATGTAGCAAAATTA
  AAAGGTTACTTAATGAGAGAAGAAGTGAAAGCCCTAGAAAAAGGTATTGAATTAGAAGAT
  GGTATGACGCAACCGGCTGAAGTGAAAGTTAAGAAACAAGATAAAGATAAAAACACAACA
  TTGGTTGAAATTACAATTACAGAAGGTCGTAATCGCCAAGTCAGAAGAATGTTCGAACAT
  TTTGGTCATCAAGTGACGAAACTGTCTCGTATTGAATATGGGCCTTTAAATGTTGTCGGT
  TTGAATGCTGGTGAAGGACGTGTATTGACGCCTCATGAAGTAAAAGTGATGCGTCATTTA
  GCAGAACATGGGAAATAA
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  738
  

⊟Protein

⊟General

• locus tag: SA1324 [new locus tag: SA_RS07510 ]
• symbol: RluB
• description: ribosomal large subunit pseudouridine synthase B
• length: 245
• theoretical pI: 9.8648
• theoretical MW: 27969
• GRAVY: -0.606122

⊟Function

• reaction:
  EC 5.4.99.-?  ExPASy
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification pseudouridine synthase (TIGR00093; EC 5.4.99.-; HMM-score: 167)
  and 6 more
  Genetic information processing Protein synthesis tRNA and rRNA base modification pseudouridine synthase, RluA family (TIGR00005; EC 5.4.99.-; HMM-score: 60.7)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair S4 domain protein YaaA (TIGR02988; HMM-score: 20.7)
  Metabolism Energy metabolism Photosynthesis photosystem II S4 domain protein (TIGR03069; HMM-score: 19.1)
  Unknown function General TlyA family rRNA methyltransferase/putative hemolysin (TIGR00478; HMM-score: 17.4)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS4 (TIGR01017; HMM-score: 17.1)
  Genetic information processing Protein synthesis tRNA and rRNA base modification pseudouridine synthase Rlu family protein, TIGR01621 (TIGR01621; EC 5.4.99.-; HMM-score: 16.3)
• TheSEED  :
  • LSU rRNA pseudouridine(2605) synthase (EC 5.4.99.22)
  Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Two cell division clusters relating to chromosome partitioning  Ribosomal large subunit pseudouridine synthase B (EC 4.2.1.70)
• PFAM:
  PseudoU_synth (CL0649) PseudoU_synth_2; RNA pseudouridylate synthase (PF00849; HMM-score: 82.9)
  and 7 more
  S4 (CL0492) S4; S4 domain (PF01479; HMM-score: 50.2)
  S4_2; S4 domain (PF13275; HMM-score: 25.4)
  TPR (CL0020) PPR_long; Pentacotripeptide-repeat region of PRORP (PF17177; HMM-score: 17.7)
  no clan defined PX; PX domain (PF00787; HMM-score: 15)
  SUKH (CL0526) SUKH-3; SUKH-3 immunity protein (PF14433; HMM-score: 15)
  tRNA_bind_arm (CL0298) Val_tRNA-synt_C; Valyl tRNA synthetase tRNA binding arm (PF10458; HMM-score: 14.3)
  HTH (CL0123) DUF4364; Domain of unknown function (DUF4364) (PF14277; HMM-score: 13.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010895
  • TAT(Tat/SPI): 0.001817
  • LIPO(Sec/SPII): 0.002516
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927074 NCBI
• RefSeq: NP_374607 NCBI
• UniProt: A0A0H3JL16 UniProt

⊟Protein sequence

• MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGTKVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPVGRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELEDGMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNVVGLNAGEGRVLTPHEVKVMRHLAEHGK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: rluB < SA1325 < SA1326

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications