NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

organism: Staphylococcus aureus COL

locus tag: SACOL_RS06585 [old locus tag: SACOL1290 ]

pan locus tag^?: SAUPAN003572000

symbol: SACOL_RS06585

pan gene symbol^?: truB

synonym:
product: tRNA pseudouridine(55) synthase TruB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SACOL_RS06585 [old locus tag: SACOL1290 ]
symbol: SACOL_RS06585
product: tRNA pseudouridine(55) synthase TruB
replicon: chromosome
strand: +
coordinates: 1303208..1304125
length: 918
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Location: NC_002951 (1303208..1304125) NCBI
BioCyc: SACOL_RS06585 BioCyc
MicrobesOnline: see SACOL1290

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTATAATGGGATATTACCAGTATATAAAGAGCGCGGTTTAACAAGTCATGACGTTGTA
TTCAAATTGCGTAAAATATTAAAAACTAAAAAAATAGGTCACACGGGTACGCTTGATCCC
GAAGTTGCAGGCGTGTTACCGGTATGTATAGGTAATGCAACGAGAGTTAGTGATTATGTT
ATGGATATGGGCAAAGCTTATGAAGCAACTGTATCGATAGGAAGAAGTACAACGACTGAA
GATCAAACGGGTGATACATTGGAAACAAAAGGTGTACACTCAGCAGATTTTAATAAGGAC
GATATTGACCGATTGTTAGAAAGTTTTAAAGGTATCATTGAACAAATTCCGCCGATGTAC
TCATCCGTCAAAGTAAATGGTAAAAAATTATATGAATATGCGCGTAATAATGAAACAGTT
GAAAGACCAAAGCGTAAAGTTAATATTAAAGACATTGGGCGTATATCTGAATTAGATTTT
AAAGAAAATGAGTGTCATTTTAAAATACGCGTCATCTGTGGTAAAGGTACATATATTAGA
ACGCTAGCAACTGATATTGGTGTGAAATTAGGCTTTCCGGCACATATGTCGAAATTAACA
CGAATCGAGTCTGGTGGATTTGTGTTGAAAGATAGCCTTACATTAGAACAAATAAAAGAA
CTTCATGAGCAGGATTCATTGCAAAATAAATTGTTTCCTTTAGAATATGGATTAAAGGGT
TTGCCAAGCATTAAAATTAAAGATTCGCACATAAAAAAACGTATTTTAAATGGGCAGAAA
TTTAATAAAAATGAATTTGATAACAAAATTAAAGACCAAATTGTATTTATTGATGATGAT
TCAGAAAAAGTATTAGCAATTTATATGGTACACCCTACAAAAGAATCAGAAATTAAACCT
AAAAAAGTCTTTAATTAA
60
120
180
240
300
360
420
480
540
600
660
720
780
840
900
918

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SACOL_RS06585 [old locus tag: SACOL1290 ]
symbol: SACOL_RS06585
description: tRNA pseudouridine(55) synthase TruB
length: 305
theoretical pI: 9.56269
theoretical MW: 34592.7
GRAVY: -0.48918

⊟Function[edit | edit source]

⊞⊞reaction:
EC 4.2.1.70? ExPASy
Pseudouridylate synthase Uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H₂O
EC 5.4.99.25? ExPASy
tRNA pseudouridine⁵⁵ synthase tRNA uridine⁵⁵ = tRNA pseudouridine⁵⁵
⊞⊞TIGRFAM:
Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA pseudouridine(55) synthase (TIGR00431; EC 5.4.99.25; HMM-score: 249)
and 2 more
Genetic information processing Protein synthesis tRNA and rRNA base modification putative rRNA pseudouridine synthase (TIGR00425; EC 5.4.99.-; HMM-score: 134.3)
Metabolism Transport and binding proteins Cations and iron carrying compounds tonB-system energizer ExbB (TIGR02805; HMM-score: 11.8)
TheSEED: see SACOL1290
⊞⊞PFAM:
PseudoU_synth (CL0649) TruB_N; TruB family pseudouridylate synthase (N terminal domain) (PF01509; HMM-score: 181.9)
and 2 more
TruB_C_2; tRNA pseudouridylate synthase B C-terminal domain (PF16198; HMM-score: 45.7)
no clan defined Serine_rich; Serine rich protein interaction domain (PF08824; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

⊞⊞
PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
⊞⊞
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9669
- Cytoplasmic Membrane Score: 0.0008
- Cell wall & surface Score: 0.0001
- Extracellular Score: 0.0322
LocateP:
⊞⊞
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.007617
- TAT(Tat/SPI): 0.000587
- LIPO(Sec/SPII): 0.000837
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 446204450 NCBI
RefSeq: WP_000282305 NCBI
UniProt: see SACOL1290

⊟Protein sequence[edit | edit source]

MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAGVLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDIDRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDFKENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQIKELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVFIDDDSEKVLAIYMVHPTKESEIKPKKVFN

⊟Experimental data[edit | edit source]

experimentally validated: see SACOL1290
protein localization: see SACOL1290
quantitative data / protein copy number per cell: see SACOL1290

⊟⊟interaction partners:

SACOL_RS11135	(deoA)	pyrimidine-nucleoside phosphorylase ^[1] (data from MRSA252)
SACOL_RS01040		formate acetyltransferase ^[1] (data from MRSA252)
SACOL_RS02190		GTP-binding protein YchF ^[1] (data from MRSA252)
SACOL_RS03030		50S ribosomal protein L1 ^[1] (data from MRSA252)
SACOL_RS03040		50S ribosomal protein L7/L12 ^[1] (data from MRSA252)
SACOL_RS03075		elongation factor G ^[1] (data from MRSA252)
SACOL_RS03080		elongation factor Tu ^[1] (data from MRSA252)
SACOL_RS03235		hydroxymethylpyrimidine/phosphomethylpyrimidine kinase ^[1] (data from MRSA252)
SACOL_RS04075		ribonucleotide-diphosphate reductase subunit alpha ^[1] (data from MRSA252)
SACOL_RS04840		NADH dehydrogenase ^[1] (data from MRSA252)
SACOL_RS04980		hypothetical protein ^[1] (data from MRSA252)
SACOL_RS05185		enoyl-ACP reductase ^[1] (data from MRSA252)
SACOL_RS05470		bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase ^[1] (data from MRSA252)
SACOL_RS05630		pyruvate dehydrogenase E1 component subunit alpha ^[1] (data from MRSA252)
SACOL_RS05645		dihydrolipoyl dehydrogenase ^[1] (data from MRSA252)
SACOL_RS06140		cell division protein FtsZ ^[1] (data from MRSA252)
SACOL_RS06225		carbamoyl-phosphate synthase large chain ^[1] (data from MRSA252)
SACOL_RS07525		serine/threonine dehydratase ^[1] (data from MRSA252)
SACOL_RS08905		isocitrate dehydrogenase (NADP(+)) ^[1] (data from MRSA252)
SACOL_RS08930		pyruvate kinase ^[1] (data from MRSA252)
SACOL_RS08995		universal stress protein UspA ^[1] (data from MRSA252)
SACOL_RS10045		glutamate-1-semialdehyde 2,1-aminomutase ^[1] (data from MRSA252)
SACOL_RS10945		UDP-N-acetylglucosamine 1-carboxyvinyltransferase ^[1] (data from MRSA252)
SACOL_RS11070		UDP-N-acetylglucosamine 1-carboxyvinyltransferase ^[1] (data from MRSA252)
SACOL_RS11435		Asp23/Gls24 family envelope stress response protein ^[1] (data from MRSA252)
SACOL_RS11695		30S ribosomal protein S5 ^[1] (data from MRSA252)
SACOL_RS13415		hydroxymethylglutaryl-CoA synthase ^[1] (data from MRSA252)
SACOL_RS13725		malate:quinone oxidoreductase ^[1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

regulator: SigB* see SACOL1290

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊞Other Information[edit | edit source]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ ^{Jump up to: 1.00} ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 ^1.17 ^1.18 ^1.19 ^1.20 ^1.21 ^1.22 ^1.23 ^1.24 ^1.25 ^1.26 ^1.27 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

[pubmed21166474-1] {Jump up to: 1.00} ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 ^1.17 ^1.18 ^1.19 ^1.20 ^1.21 ^1.22 ^1.23 ^1.24 ^1.25 ^1.26 ^1.27 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)

[1]

Search

Navigation menu

Links

⊟Summary[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

⊟DNA sequence[edit | edit source]

⊟Protein[edit | edit source]

⊟General[edit | edit source]

⊟Function[edit | edit source]

⊟Structure, modifications & cofactors[edit | edit source]

⊟Localization[edit | edit source]

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

⊟Summary[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

⊟DNA sequence[edit | edit source]

⊟Protein[edit | edit source]

⊟General[edit | edit source]

⊟Function[edit | edit source]

⊟Structure, modifications & cofactors[edit | edit source]

⊟Localization[edit | edit source]

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]