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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1192 [new locus tag: SACOL_RS06105 ]
  • pan locus tag?: SAUPAN003449000
  • symbol: mraW
  • pan gene symbol?: mraW
  • synonym:
  • product: S-adenosyl-methyltransferase MraW

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1192 [new locus tag: SACOL_RS06105 ]
  • symbol: mraW
  • product: S-adenosyl-methyltransferase MraW
  • replicon: chromosome
  • strand: +
  • coordinates: 1196309..1197244
  • length: 936
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    GTGTTTCATCATATCAGCGTTATGTTAAACGAAACCATTGATTATTTAAATGTAAAAGAA
    AATGGTGTGTACATTGACTGTACGCTAGGTGGAGCGGGACATGCCCTTTATTTACTAAAT
    CAATTAAATGACGACGGAAGATTAATAGCAATCGATCAAGACCAAACTGCAATTGATAAT
    GCTAAAGAGGTATTAAAGGATCATTTGCATAGGGTGACTTTTGTTCATAGCAACTTCCGT
    GAATTAACTCAAATATTAAAAGACTTAAACATTGAAAAAGTAGATGGAATTTATTACGAC
    TTGGGTGTTTCAAGCCCACAACTCGACATTCCAGAACGAGGATTCAGTTATCACCATGAC
    GCAACATTAGACATGCGTATGGACCAAACACAAGAACTAACAGCATATGAAATTGTTAAC
    AATTGGTCATATGAAGCGTTAGTGAAGATTTTTTATCGCTATGGCGAGGAGAAATTTTCA
    AAACAGATAGCTCGAAGAATCGAAGCACATCGCGAACAACAACCAATAACAACAACATTA
    GAATTAGTTGACATTATAAAAGAAGGTATTCCTGCAAAAGCAAGAAGAAAAGGCGGACAT
    CCTGCAAAACGAGTATTTCAAGCACTACGAATTGCAGTAAACGATGAATTGTCAGCTTTT
    GAAGATTCAATAGAACAAGCGATTGAATTAGTGAAAGTAGATGGCAGGATTTCGGTAATC
    ACTTTCCATTCTTTAGAAGATCGTTTATGTAAACAGGTGTTCCAAGAATATGAAAAAGGT
    CCAGAGGTACCAAGAGGATTACCAGTTATACCAGAAGCATATACACCTAAGTTAAAGCGT
    GTTAATCGTAAACCGATTACCGCTACAGAAGAAGATTTAGATGACAATAACAGAGCACGA
    AGCGCGAAATTACGTGTAGCTGAAATACTTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    936

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1192 [new locus tag: SACOL_RS06105 ]
  • symbol: MraW
  • description: S-adenosyl-methyltransferase MraW
  • length: 311
  • theoretical pI: 5.97721
  • theoretical MW: 35709.3
  • GRAVY: -0.454984

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.1.1.199?  ExPASy
    16S rRNA (cytosine1402-N4)-methyltransferase S-adenosyl-L-methionine + cytosine1402 in 16S rRNA = S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
  • ⊞TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (cytosine(1402)-N(4))-methyltransferase (TIGR00006; EC 2.1.1.199; HMM-score: 409.6)
    and 1 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone demethylmenaquinone methyltransferase (TIGR02752; EC 2.1.1.163; HMM-score: 14.6)
  • ⊞TheSEED  :
    • rRNA small subunit methyltransferase H
    Bacterial Cell Division  rRNA small subunit methyltransferase H
    and 1 more
    RP Bacterial Cell Division  rRNA small subunit methyltransferase H
  • ⊞PFAM:
    NADP_Rossmann (CL0063) Methyltransf_5; MraW methylase family (PF01795; HMM-score: 470.7)
    and 10 more
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 30.2)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 21.8)
    rRNA_methylase; Putative rRNA methylase (PF06962; HMM-score: 20.3)
    Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 18.6)
    Methyltransf_24; Methyltransferase domain (PF13578; HMM-score: 17.7)
    Methyltransf_3; O-methyltransferase (PF01596; HMM-score: 17.4)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 15.3)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 14.4)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 13.8)
    GCD14; tRNA methyltransferase complex GCD14 subunit (PF08704; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9992
    • Cytoplasmic Membrane Score: 0.0004
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0004
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003934
    • TAT(Tat/SPI): 0.000098
    • LIPO(Sec/SPII): 0.000233
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDNAKEVLKDHLHRVTFVHSNFRELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFSKQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 179 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 7.6 h [5]

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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