⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA2194 [new locus tag: SA_RS12580 ]
pan locus tag^?: SAUPAN005938000
symbol: SA2194
pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2194 [new locus tag: SA_RS12580 ]
• symbol: SA2194
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2464159..2465706
• length: 1548
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125122 NCBI
• RefSeq: NP_375517 NCBI
• BioCyc: see SA_RS12580
• MicrobesOnline: 104543 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAAAGAAATTAGGTATGTTACTTCTTGTACCAGCCGTAACTTTATCATTAGCCGCA
  TGTGGGAATGATGATGGAAAAGATAAAGATGGCAAGGTAACAATTAAAACGACAGTTTAT
  CCATTGCAATCATTTGCAGAGCAAATTGGTGGAAAACACGTGAAGGTATCATCAATCTAT
  CCAGCAGGGACAGATTTACATAGCTATGAACCAACACAAAAAGATATATTAAGTGCAAGC
  AAGTCAGACTTGTTTATGTATACAGGGGATAATTTAGATCCGGTTGCTAAGAAAGTTGCA
  TCTACTATCAAAGATAAAGATAAAAAACGGTCTTTAGAGGATAAATTAGATAAAGCAAAG
  CTTTTAACTGATCAACACGAGCATGGTGAAGAGCATGAACATGAGGGGCATGATCATGAG
  AAAGAAGAACATCATCATCATGGTGGATATGATCCACACGTATGGTTAGATCCTAAAATT
  AACCAAACTTTCGCTAAAGAAATTAAAGATGAATTAGTGAAAAAAGATCCAAAACATAAA
  GATGACTATGAGAAAAACTACAAAAAATTAAACGACGATCTTAAGAAAATTGATAACGAT
  ATGAAGCAAGTTACAAAAGATAAGCAAGGTAATGCAGTATTTATTTCACATGAATCAATT
  GGATACTTAGCTGATCGTTATGGTTTTGTTCAAAAAGGTATTCAAAATATGAATGCTGAA
  GATCCATCACAAAAAGAATTAACTAAAATTGTTAAAGAAATTAGAGATAGCAATGCTAAA
  TATATTCTTTACGAAGATAATGTTGCGAATAAAGTGACTGAAACAATTCGTAAAGAAACA
  GATGCGAAGCCTTTAAAATTCTACAACATGGAGTCTTTAAATAAAGAACAACAGAAAAAA
  GATAATATTACGTATCAATCATTAATGAAATCGAATATTGAAAATATCGGTAAAGCTTTA
  GACAGTGGTGTTAAAGTGAAAGACGACAAAGCTGAAAGTAAACACGATAAAGCAATTTCT
  GATGGGTATTTTAAAGATGAGCAAGTTAAAGACCGTGAATTAAGCGATTATGCTGGTGAA
  TGGCAATCTGTTTACCCTTACTTAAAAGACGGTACGCTTGATGAAGTGATGGAACATAAA
  GCTGAAAATGATCCGAAGAAATCTGCTAAAGATTTAAAAGCTTATTATGACAAAGGATAT
  AAAACTGATATTACTAACATTGATATAAAAGGAAATGAAATTACATTTACTAAAGATGGT
  AAGAAACACACTGGTAAATATGAATACAATGGTAAGAAAACATTGAAATATCCTAAAGGT
  AACCGTGGCGTGAGATTTATGTTTAAATTGGTCGATGGTAATGATAAAGACTTACCGAAA
  TTCATCCAATTTAGCGATCACAACATTGCACCTAAAAAGGCAGAACACTTCCATATCTTT
  ATGGGTAATGATAATGACGCGTTATTAAAAGAAATGGATAACTGGCCAACATATTATCCT
  TCAAAATTAAATAAAGACCAAATCAAAGAAGAAATGTTAGCACATTAA
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  1548
  

⊟Protein

⊟General

• locus tag: SA2194 [new locus tag: SA_RS12580 ]
• symbol: SA2194
• description: hypothetical protein
• length: 515
• theoretical pI: 7.2821
• theoretical MW: 59145.2
• GRAVY: -1.06408

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Unknown substrate anchored repeat ABC transporter, substrate-binding protein (TIGR03772; HMM-score: 84.9)
• TheSEED  :
  • Zinc ABC transporter, substrate-binding lipoprotein AdcA
• ⊞⊞PFAM:
  Calycin (CL0116) ZinT; ZinT (YodA) periplasmic lipocalin-like zinc-recruitment (PF09223; HMM-score: 275.8)
  Chelatase (CL0043) ZnuA; Zinc-uptake complex component A periplasmic (PF01297; HMM-score: 238.4)
  and 5 more
  no clan defined DUF4971; Domain of unknown function (DUF4971) (PF16341; HMM-score: 16.9)
  LPD3; Large polyvalent protein-associated domain 3 (PF18798; HMM-score: 14.3)
  sPC4_like (CL0609) T4_y05I_C; y05I-like putative transcription factor C-terminal domain (PF24448; HMM-score: 9.8)
  no clan defined Hia_Tpr_ring_dom; Trimeric autotransporter adhesin, Trp ring domain (PF22414; HMM-score: 9.2)
  DUF4460; Domain of unknown function (DUF4460) (PF14687; HMM-score: 8.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.68
  • Cellwall Score: 0.17
  • Extracellular Score: 0.16
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0007
  • Cytoplasmic Membrane Score: 0.8597
  • Cell wall & surface Score: 0.0724
  • Extracellular Score: 0.0672
• ⊞⊞LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: LSLAACG
• ⊞⊞SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
  • SP(Sec/SPI): 0.00062
  • TAT(Tat/SPI): 0.000068
  • LIPO(Sec/SPII): 0.999149
  • Cleavage Site: CS pos: 20-21. LAA-CG. Pr: 0.9997
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927984 NCBI
• RefSeq: NP_375517 NCBI
• UniProt: A0A0H3JND0 UniProt

⊟Protein sequence

• MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQSFAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVASTIKDKDKKRSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHGGYDPHVWLDPKINQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISHESIGYLADRYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTETIRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAESKHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKDLKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFKLVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQIKEEMLAH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• ⊞⊞regulator: Zur* (repression) regulon

  Zur*

  (TF)

  important in Zinc homeostasis; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications