⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_1610 [new locus tag: NWMN_RS09025 ]
pan locus tag^?: SAUPAN004368000
symbol: NWMN_1610
pan gene symbol^?: nifZ
• synonym:
• product: aminotransferase, class V

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1610 [new locus tag: NWMN_RS09025 ]
• symbol: NWMN_1610
• product: aminotransferase, class V
• replicon: chromosome
• strand: -
• coordinates: 1787327..1788466
• length: 1140
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332537 NCBI
• RefSeq: YP_001332644 NCBI
• BioCyc:
• MicrobesOnline: 3707162 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  TTGATATATCTAGATAATGCGGCAACGACGAAAGCATTTGAAGAAGTGTTAGATACTTAT
  TTAAAAGTAAATCAATCAATGTATTATAATCCGAATAGTCCGCATAAAGCTGGTTTGCAG
  GCAAATCAATTACTACAACAAGCAAAAACCCAAATTAATGCAATGATTAATTCAAAAACA
  AATTATGATGTTGTATTCACTAGTGGTGCAACTGAATCCAATAATCTTGCTTTAAAAGGT
  ATTGCCTATCGTAAATTTGATACAGCGAAGGAAATAATTACATCCGTGTTAGAGCATCCG
  TCCGTATTAGAGGTTGTAAGATATTTGGAAGCACACGAAGGATTTAAAGTTAAATATGTT
  GATGTAAAGAAAGATGGCAGTATTAACTTAGAACACTTCAAAGAATTAATGTCAGACAAA
  GTCGGTTTAGTAACATGTATGTATGTAAATAATGTAACTGGACAAATACAGCCTATTCCA
  CAAATGGCTAAAGTTATAAAAAATTATCCTAAGGCACATTTTCATGTAGATGCGGTTCAA
  GCATTCGGCAAAATTTCAATGGATCTCAATAACATAGATAGTATTAGTTTAAGTGGACAC
  AAGTTTAATGGTTTAAAAGGACAAGGCGTCTTACTTGTAAATCACATTCAAAATGTTGAA
  CCAACTGTCCATGGTGGTGGTCAAGAATATGGCGTTAGAAGTGGAACAGTTAATTTGCCA
  AATGATATTGCAATGGTTAAAGCGATGAAGATAGCTAATGAAAACTTTGAAGCATTGAAT
  GCATTTGTTACTGAGTTAAATAATGACGTCCGTCAATTTTTAAATAAATATCATGGAGTT
  TATATTAATTCTTCAACTTCAGGTTCACCATTCGTTTTAAATATTAGTTTTCCTGGCGTA
  AAAGGTGAAGTATTAGTTAATGCTTTTTCAAAATATGACATTATGATATCTACGACAAGT
  GCTTGTTCATCTAAACGTAATAAATTAAATGAAGTATTGGCTGCAATGGGATTATCAGAC
  AAATCTATTGAAGGTAGTATAAGATTATCATTTGGGGCTACTACAACTAAAGAAGATATA
  GCGAGGTTTAAAGAAATATTTATCATCATTTATGAGGAAATTAAGGAGTTGCTAAAATAA
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⊟Protein

⊟General

• locus tag: NWMN_1610 [new locus tag: NWMN_RS09025 ]
• symbol: NWMN_1610
• description: aminotransferase, class V
• length: 379
• theoretical pI: 8.1916
• theoretical MW: 41991.8
• GRAVY: -0.156464

⊟Function

• ⊞⊞TIGRFAM:
  cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 310.8)
  Genetic information processing DNA metabolism Restriction/modification cysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 297.6)
  Genetic information processing Protein synthesis tRNA and rRNA base modification cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 281.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 281.6)
  cysteine desulfurase, NifS family (TIGR03403; EC 2.8.1.7; HMM-score: 250.1)
  and 7 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 139)
  Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 132.1)
  Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01976; HMM-score: 86.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, catalytic subunit CsdA (TIGR03392; EC 2.8.1.7; HMM-score: 76.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs ergothioneine biosynthesis PLP-dependent enzyme EgtE (TIGR04343; EC 4.4.-.-; HMM-score: 43.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other tyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 28)
  Metabolism Central intermediary metabolism Phosphorus compounds 2-aminoethylphosphonate--pyruvate transaminase (TIGR02326; EC 2.6.1.37; HMM-score: 25.4)
• ⊞⊞TheSEED  :
  • Cysteine desulfurase (EC 2.8.1.7)
  Amino Acids and Derivatives Alanine, serine, and glycine Alanine biosynthesis  Cysteine desulfurase (EC 2.8.1.7)
  and 2 more
  RNA Metabolism RNA processing and modification tRNA modification Archaea  Cysteine desulfurase (EC 2.8.1.7)
  Sulfur Metabolism Organic sulfur assimilation At5g37530 (CsdL protein family)  Cysteine desulfurase (EC 2.8.1.7)
• ⊞⊞PFAM:
  PLP_aminotran (CL0061) Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 239.5)
  and 6 more
  Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 29.8)
  DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 21.7)
  EF_hand (CL0220) EF-hand_1; EF hand domain (PF00036; HMM-score: 15.9)
  PLP_aminotran (CL0061) Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 15.1)
  no clan defined DUF7659; Family of unknown function (DUF7659) (PF24692; HMM-score: 10.7)
  DUF4473; Domain of unknown function (DUF4473) (PF14747; HMM-score: 8.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: pyridoxal 5'-phosphate
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9705
  • Cytoplasmic Membrane Score: 0.0004
  • Cell wall & surface Score: 0.0008
  • Extracellular Score: 0.0282
• ⊞⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.012625
  • TAT(Tat/SPI): 0.000704
  • LIPO(Sec/SPII): 0.001154
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221822 NCBI
• RefSeq: YP_001332644 NCBI
• UniProt: A0A0H3KDB3 UniProt

⊟Protein sequence

• MIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQANQLLQQAKTQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEHPSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQPIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNHIQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQFLNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNEVLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1608 < thiI < NWMN_1610

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications