PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_0177 [new locus tag: NWMN_RS00990 ]
pan locus tag^?: SAUPAN001110000
symbol: NWMN_0177
pan gene symbol^?: glcC
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0177 [new locus tag: NWMN_RS00990 ]
• symbol: NWMN_0177
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 229587..231122
• length: 1536
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332076 NCBI
• RefSeq: YP_001331212 NCBI
• BioCyc:
• MicrobesOnline: 3705708 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTTGATGAAATCTTTATTTGAAAAAGCACAGCAGTTCGGCAAGTCCTTTATGTTACCT
  ATCGCAATCTTACCAGCTGCAGGTCTATTGTTGGGTATCGGTGGTGCATTAAGTAATCCA
  AACACCGTTAAAGCATACCCTATTTTAGATATTACCTTATTACAAAATATTTTTACATTA
  ATGTCAGCTGCAGGTAGTATTGTTTTCCAAAATTTACCGGTCATCTTTGCAATTGGTGTC
  GCAATCGGATTATCTAGAAGCGATAAAGGTACTGCAGGTTTAGCTGCGCTGCTCGGTTTC
  TTAATTATGAACGCAACTATGAATGGCTTATTAACTATCACGGGCACATTGGCAAAAGAT
  CAGCTTGCACAAAATGGACAAGGCATGGTGCTCGGTATACAAACGGTTGAAACCGGTGTT
  TTTGGCGGGATTATCACAGGTATTATGACCGCAATACTTCACAACAAATATCACAAAGTG
  GTATTACCACCGTATTTAGGTTTCTTTGGTGGCTCTAGATTTGTCCCTATTGTCACAGCA
  TTTGCCGCAATCTTTTTAGGTGTATTGATGTTTTTCATTTGGCCAAGCATACAAGCCGGC
  ATTTATCATGTTGGTGGATTTGTAACGAAAACAGGTGCCATCGGTACTTTTGTTTATGGC
  TTCATCTTAAGATTGTTAGGTCCACTCGGTTTACACCATATTTTTTACTTACCGTTTTGG
  CAGACGGCACTTGGTGGTACTTTAGAAGTCAAAGGGCACTTAGTTCAAGGTACGCAGAAC
  ATCTTCTTTGCTCAACTTGGTGATCCAGATGTGACGAAGTATTATTCAGGTGTGTCACGC
  TTTATGTCAGGCCGTTTTATTACGATGATGTTCGGCTTATGTGGTGCCGCACTTGCAATT
  TATCACACAGCTAAACCTGAACATAAAAAAGTTGTCGGCGGTTTAATGTTATCCGCTGCA
  CTCACTTCATTTTTAACAGGTATTACCGAACCTTTAGAGTTTAGTTTCTTGTTTGTCGCA
  CCTATTCTTTATGTAATCCATGCCTTCTTTGATGGATTAGCATTTATGATGGCAGACATT
  TTCAACATTACAATTGGTCAAACCTTCAGTGGAGGCTTTATCGATTTCTTACTCTTTGGT
  GTGCTACAAGGTAATAGTAAAACAAACTACCTATACGTCATACCTATTGGAATTGTGTGG
  TTCTGTTTGTATTACATCGTTTTCAGATTCTTAATTACGAAATTTAATTTCAAAACACCT
  GGTCGAGAAGATAAAGCTGCAGCACAACAAGTTGAGGCTACTGAAAGAGCACAAACTATT
  GTTGCTGGTTTGGGAGGCAAAGATAACATTGAAATCGTTGACTGTTGTGCAACGAGACTA
  CGCGTCACACTTCATCAAAATGACAAAGTCGATAAAGTATTACTCGAAAGTACTGGTGCC
  AAAGGTGTAATCCAGCAAGGCACTGGTGTGCAAGTAATTTATGGGCCTCACGTTACAGTT
  ATCAAAAATGAAATTGAAGAATTGCTCGGGGATTAA
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  1536
  

⊟Protein

⊟General

• locus tag: NWMN_0177 [new locus tag: NWMN_RS00990 ]
• symbol: NWMN_0177
• description: hypothetical protein
• length: 511
• theoretical pI: 8.96532
• theoretical MW: 55177.7
• GRAVY: 0.622309

⊟Function

• ⊞TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 591.6)
  and 11 more
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 463.4)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 450.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 314.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 285.1)
  Signal transduction Signal transduction PTS PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 285.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 268.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.4)
  Signal transduction Signal transduction PTS PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.4)
  PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 70)
  PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 44.1)
  PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 43.5)
• ⊞TheSEED  :
  • PTS system, N-acetylmannosamine-specific IIB component
  • PTS system, N-acetylmannosamine-specific IIC component
  Carbohydrates Di- and oligosaccharides Maltose and Maltodextrin Utilization  PTS system, maltose and glucose-specific IIB component (EC 2.7.1.69)
  and 1 more
  Carbohydrates Di- and oligosaccharides Maltose and Maltodextrin Utilization  PTS system, maltose and glucose-specific IIC component (EC 2.7.1.69)
• ⊞PFAM:
  PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 298.2)
  and 1 more
  no clan defined PTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 52.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• ⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 1
  • Cell wall & surface Score: 0
  • Extracellular Score: 0
• ⊞LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.414729
  • TAT(Tat/SPI): 0.084007
  • LIPO(Sec/SPII): 0.046272
• predicted transmembrane helices (TMHMM): 11

⊟Accession numbers

• GI: 151220389 NCBI
• RefSeq: YP_001331212 NCBI
• UniProt: A0A0H3KA84 UniProt

⊟Protein sequence

• MLMKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAYPILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMNATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVLPPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYGFILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGVSRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSFLFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVIPIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEIVDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLGD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• ⊞regulator: NanR* (repression) regulon

  NanR*

  (TF)

  important in Sialic acid utilization, N-acetylglucosamine utilization; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications