PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS00990 [old locus tag: NWMN_0177 ]
• pan locus tag^?: SAUPAN001110000
• symbol: NWMN_RS00990
• pan gene symbol^?: glcC
• synonym:
• product: PTS glucose transporter subunit IIB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS00990 [old locus tag: NWMN_0177 ]
• symbol: NWMN_RS00990
• product: PTS glucose transporter subunit IIB
• replicon: chromosome
• strand: -
• coordinates: 229587..231116
• length: 1530
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (229587..231116) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0177

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAATCTTTATTTGAAAAAGCACAGCAGTTCGGCAAGTCCTTTATGTTACCTATCGCA
  ATCTTACCAGCTGCAGGTCTATTGTTGGGTATCGGTGGTGCATTAAGTAATCCAAACACC
  GTTAAAGCATACCCTATTTTAGATATTACCTTATTACAAAATATTTTTACATTAATGTCA
  GCTGCAGGTAGTATTGTTTTCCAAAATTTACCGGTCATCTTTGCAATTGGTGTCGCAATC
  GGATTATCTAGAAGCGATAAAGGTACTGCAGGTTTAGCTGCGCTGCTCGGTTTCTTAATT
  ATGAACGCAACTATGAATGGCTTATTAACTATCACGGGCACATTGGCAAAAGATCAGCTT
  GCACAAAATGGACAAGGCATGGTGCTCGGTATACAAACGGTTGAAACCGGTGTTTTTGGC
  GGGATTATCACAGGTATTATGACCGCAATACTTCACAACAAATATCACAAAGTGGTATTA
  CCACCGTATTTAGGTTTCTTTGGTGGCTCTAGATTTGTCCCTATTGTCACAGCATTTGCC
  GCAATCTTTTTAGGTGTATTGATGTTTTTCATTTGGCCAAGCATACAAGCCGGCATTTAT
  CATGTTGGTGGATTTGTAACGAAAACAGGTGCCATCGGTACTTTTGTTTATGGCTTCATC
  TTAAGATTGTTAGGTCCACTCGGTTTACACCATATTTTTTACTTACCGTTTTGGCAGACG
  GCACTTGGTGGTACTTTAGAAGTCAAAGGGCACTTAGTTCAAGGTACGCAGAACATCTTC
  TTTGCTCAACTTGGTGATCCAGATGTGACGAAGTATTATTCAGGTGTGTCACGCTTTATG
  TCAGGCCGTTTTATTACGATGATGTTCGGCTTATGTGGTGCCGCACTTGCAATTTATCAC
  ACAGCTAAACCTGAACATAAAAAAGTTGTCGGCGGTTTAATGTTATCCGCTGCACTCACT
  TCATTTTTAACAGGTATTACCGAACCTTTAGAGTTTAGTTTCTTGTTTGTCGCACCTATT
  CTTTATGTAATCCATGCCTTCTTTGATGGATTAGCATTTATGATGGCAGACATTTTCAAC
  ATTACAATTGGTCAAACCTTCAGTGGAGGCTTTATCGATTTCTTACTCTTTGGTGTGCTA
  CAAGGTAATAGTAAAACAAACTACCTATACGTCATACCTATTGGAATTGTGTGGTTCTGT
  TTGTATTACATCGTTTTCAGATTCTTAATTACGAAATTTAATTTCAAAACACCTGGTCGA
  GAAGATAAAGCTGCAGCACAACAAGTTGAGGCTACTGAAAGAGCACAAACTATTGTTGCT
  GGTTTGGGAGGCAAAGATAACATTGAAATCGTTGACTGTTGTGCAACGAGACTACGCGTC
  ACACTTCATCAAAATGACAAAGTCGATAAAGTATTACTCGAAAGTACTGGTGCCAAAGGT
  GTAATCCAGCAAGGCACTGGTGTGCAAGTAATTTATGGGCCTCACGTTACAGTTATCAAA
  AATGAAATTGAAGAATTGCTCGGGGATTAA
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⊟Protein

⊟General

• locus tag: NWMN_RS00990 [old locus tag: NWMN_0177 ]
• symbol: NWMN_RS00990
• description: PTS glucose transporter subunit IIB
• length: 509
• theoretical pI: 8.96532
• theoretical MW: 54933.4
• GRAVY: 0.613556

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 591.6)
  and 11 more
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 463.4)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 450.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 314.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 285.1)
  Signal transduction Signal transduction PTS PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 285.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 268.4)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.4)
  Signal transduction Signal transduction PTS PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.4)
  PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 69.9)
  PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 44.1)
  PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 43.4)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 312)
  and 1 more
  no clan defined PTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 49.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 43 aa
  • SP(Sec/SPI): 0.578981
  • TAT(Tat/SPI): 0.088154
  • LIPO(Sec/SPII): 0.051874
  • Cleavage Site: CS pos: 43-44. VKA-YP. Pr: 0.4433
• predicted transmembrane helices (TMHMM): 11

⊟Accession numbers

• GI: 446760948 NCBI
• RefSeq: WP_000838204 NCBI
• UniProt: see NWMN_0177

⊟Protein sequence

• MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAYPILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMNATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVLPPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYGFILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGVSRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSFLFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVIPIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEIVDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLGD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: NanR* see NWMN_0177

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications