⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS01580 [old locus tag: SACOL0313 ]
pan locus tag^?: SAUPAN001240000
symbol: SACOL_RS01580
pan gene symbol^?: nanK
• synonym:
• product: ROK family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS01580 [old locus tag: SACOL0313 ]
• symbol: SACOL_RS01580
• product: ROK family protein
• replicon: chromosome
• strand: +
• coordinates: 348156..349016
• length: 861
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (348156..349016) NCBI
• BioCyc: SACOL_RS01580 BioCyc
• MicrobesOnline: see SACOL0313

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  GTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAG
  CAATTGAATATGTTTGACTATCAACAAATATCAACGCCGGACAACAAAAGTGAGCTTATT
  ACTGACAAAGTATATGAGATTGTAACAGGATATATGAAGCAATATCAGTTGATCCAACCT
  GTCATAGGTATTTCATCAGCAGGCGTTGTTGATGAACAAAAAGGCGAAATTGTATACGCA
  GGGCCAACCATTCCGAATTATAAAGGTACTAATTTTAAGCGATTATTAAAATCACTGTCT
  CCTTATGTCAAAGTAAAAAATGATGTAAACGCTGCATTACTAGGCGAATTGAAATTACAT
  CAATATCAAGCAGAACGGATCTTTTGTATGACGCTTGGTACAGGCATTGGGGGTGCGTAC
  AAGAATAATCAAGGTCATATTGATAATGGTGAGCTTCATAAGGCAAATGAAGTTGGGTAT
  TTATTGTATCGTCCAACTGAAAATACAACGTTTGAGCAACGTGCTGCAACGAGTGCATTG
  AAAAAGCGCATGATTGCCGGAGGATTTACGAGAAGCACACATGTGCCAGTATTGTTTGAA
  GCAGCTGAAGAAGGTGATGATATTGCAAAACAAATATTGAATGAGTGGGCAGAAGATGTA
  GCAGAAGGGATTGCCCAAATACAGGTCATGTATGATCCAGGGCTTATATTAATTGGGGGC
  GGTATATCTGAACAAGGAGATAATCTCATTAAATATATCGAGCCGAAAGTTGCACACTAT
  TTACCAAAAGACTATGTTTATGCACCAATACAAACGACTAAGAGTAAAAATGATGCAGCA
  TTATATGGCTGTTTGCAATGA
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⊟Protein

⊟General

• locus tag: SACOL_RS01580 [old locus tag: SACOL0313 ]
• symbol: SACOL_RS01580
• description: ROK family protein
• length: 286
• theoretical pI: 5.69776
• theoretical MW: 31682.9
• GRAVY: -0.262238

⊟Function

• TIGRFAM:
  Unknown function General ROK family protein (putative glucokinase) (TIGR00744; HMM-score: 112.5)
• TheSEED: see SACOL0313
• ⊞⊞PFAM:
  Actin_ATPase (CL0108) ROK; ROK family (PF00480; HMM-score: 123.6)
  and 3 more
  BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 23.2)
  Pan_kinase; Type III pantothenate kinase (PF03309; HMM-score: 15.4)
  FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 13.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9977
  • Cytoplasmic Membrane Score: 0.0011
  • Cell wall & surface Score: 0.0003
  • Extracellular Score: 0.0009
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004312
  • TAT(Tat/SPI): 0.000111
  • LIPO(Sec/SPII): 0.000616
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446213590 NCBI
• RefSeq: WP_000291445 NCBI
• UniProt: see SACOL0313

⊟Protein sequence

• MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKVYEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPYVKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGYLLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAEDVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSKNDAALYGCLQ

⊟Experimental data

• experimentally validated: see SACOL0313
• protein localization: see SACOL0313
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA, NanR* see SACOL0313

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications