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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2162 [new locus tag: SA_RS12410 ]
  • pan locus tag?: SAUPAN005890000
  • symbol: SA2162
  • pan gene symbol?: iruO
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2162 [new locus tag: SA_RS12410 ]
  • symbol: SA2162
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2428800..2429795
  • length: 996
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    TTGTATGCAAGCTTTTATGCTGGATTGCGAGATATGTCTGTAAGATTGATAGATGTTCAA
    TCTGAATTAGGGGGTAAGATGAGAATTTACCCTGAAAAAATTATTTGGGATATTGGCGGC
    ATTGCGCCAAAACCTTGTCATGAAATTTTAAAAGATACAATTAAGCAAGGATTATATTTT
    AAACCGGAAGTTCATTTGAATGAACGTGTGGTAGATATTAGGAAAAAGGCAGAACGTCAT
    TTCGAAGTTGAAACAGAAGCGGGAGAAATATATACATCGAAAGCAGTTATCATTGCAATT
    GGTGCGGGTATTATTAATCCAAAACAATTAGATGTTAAAGGTGTAGAGAGATATCAATTA
    ACTAATTTACATTATGTTGTACAAAGTTACAGACGTTTCAAAGATAAAGATGTATTAATA
    TCAGGTGGAGGAAATACAGCATTAGATTGGGCGCATGATATTGCCAAGATTGCTAAAAGC
    GTGACAGTTGTTTATCGAAAAGAAGATGTAAGTGGTCATGAAGCGATGAAAACGTTGGTG
    ACAGATTTAAATGTGAAACTATGCCCAAAAACACGTATTAAATACTTGGTCGGCAATGAT
    GATGAAACGCATATAAGTGAAGTTGTTTTGGAACATGTTGAAAGTGGCGATAGGCATACA
    GTTAAATTTGACGATGTCATTATTAGTCATGGGTTTGATCGTTGTAATACATTATTGAGT
    GAAACATCTTCAAAGCTAGATATGCATGATGATTGTCGTGTTAAAGGATTTGGTAATACG
    ACAACTAGTATACCTGGTATCTATGCGTGTGGAGATATTGTTTATCATGATGCAAAATCA
    CATCTAATTGCAAGTGCATTTAGTGATGGTGCAAATGCAGCGAACCTTGCTAAAACATAT
    ATCCAACCAGATGCGAATGCAGAGGGTTATGTTTCAAGTCATCATGAAGTGTTTAAAGAA
    GCGAATAAGACTATTGTAAATAAACATTTATACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    996

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2162 [new locus tag: SA_RS12410 ]
  • symbol: SA2162
  • description: hypothetical protein
  • length: 331
  • theoretical pI: 6.95906
  • theoretical MW: 37033.9
  • GRAVY: -0.305136

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 1.18.1.2?  ExPASy
    Ferredoxin--NADP+ reductase 2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH
  • ⊞TIGRFAM:
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 108.3)
    and 18 more
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 77.1)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 77.1)
    Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 66.3)
    putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 66)
    Metabolism Amino acid biosynthesis Glutamate family glutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 46)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 42)
    mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 36.5)
    Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 35)
    Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 34.4)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 28.9)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 28.3)
    putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 27.9)
    thioredoxin and glutathione reductase (TIGR01438; EC 1.6.4.-; HMM-score: 26.7)
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 26.3)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 21)
    Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 19.7)
    flavin-dependent oxidoreductase, MSMEG_0569 family (TIGR04046; HMM-score: 15.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 12.5)
  • ⊞TheSEED  :
    • Thioredoxin reductase (EC 1.8.1.9)
    Nucleosides and Nucleotides Pyrimidines pyrimidine conversions  Thioredoxin reductase (EC 1.8.1.9)
    and 1 more
    Sulfur Metabolism Sulfur Metabolism - no subcategory Thioredoxin-disulfide reductase  Thioredoxin reductase (EC 1.8.1.9)
  • ⊞PFAM:
    NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 89.5)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 77.2)
    and 9 more
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 40.7)
    Lys_Orn_oxgnase; L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase (PF13434; HMM-score: 28.7)
    HI0933_like; HI0933-like protein Rossmann domain (PF03486; HMM-score: 20.5)
    Amino_oxidase; Flavin containing amine oxidoreductase (PF01593; HMM-score: 20.1)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 16.9)
    FMO-like; Flavin-binding monooxygenase-like (PF00743; HMM-score: 14.7)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 14.3)
    NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 13)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: FAD
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.876
    • Cytoplasmic Membrane Score: 0.0856
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0384
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005124
    • TAT(Tat/SPI): 0.000241
    • LIPO(Sec/SPII): 0.001734
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYASFYAGLRDMSVRLIDVQSELGGKMRIYPEKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGEIYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTALDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHISEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTSIPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEANKTIVNKHLY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: Fur* (repression) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Slade A Loutet, Marek J Kobylarz, Crystal H T Chau, Michael E P Murphy
IruO is a reductase for heme degradation by IsdI and IsdG proteins in Staphylococcus aureus.
J Biol Chem: 2013, 288(36);25749-59
[PubMed:23893407] [WorldCat.org] [DOI] (I p)

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