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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002341
  • pan locus tag?: SAUPAN005890000
  • symbol: JSNZ_002341
  • pan gene symbol?: iruO
  • synonym:
  • product: NAD(P)/FAD-dependent oxidoreductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002341
  • symbol: JSNZ_002341
  • product: NAD(P)/FAD-dependent oxidoreductase
  • replicon: chromosome
  • strand: -
  • coordinates: 2339162..2340196
  • length: 1035
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAAGACGTTACAATCATTGGTGGCGGTCCGTCTGGTTTGTATGCAAGCTTTTATGCT
    GGATTGCGAGATATGTCTGTAAGATTGATAGATGTTCAATCTGAATTAGGGGGTAAGATG
    AGAATTTACCCTGAAAAAATTATTTGGGATATTGGCGGCATTGCGCCAAAACCTTGTCAT
    GAAATTTTAAAAGATACAATTAAGCAAGGATTATATTTTAAACCGGAAGTTCATTTGAAT
    GAACGTGTGGTAGATATTAGGAAAAAGGCAGAACGTCATTTCGAAGTTGAAACAGAAGCG
    GGAGAAATATATACATCGAAAGCAGTTATCATTGCAATTGGTGCGGGTATTATTAATCCA
    AAACAATTAGATGTTAAAGGTGTAGAGAGATATCAATTAACTAATTTACATTATGTTGTA
    CAAAGTTACAGACGTTTCAAAGATAAAGATGTATTAATATCAGGTGGAGGAAATACAGCA
    TTGGATTGGGCGCATGATATTGCCAAGATTGCTAAAAGCGTGACAGTTGTTTATCGAAAA
    GAAGATGTAAGTGGTCATGAAGCGATGAAAACGTTGGTGACAGATTTAAATGTGAAACTA
    TGCCCAAAAACACGTATTAAATACTTGGTCGGCAATGATGATGAAACGCATATAAGTGAA
    GTTGTTTTGGAACATGTTGAAAGTGGCGATACGCATACAGTTAAATTTGACGATGTCATT
    ATTAGTCATGGGTTTGATCGTTGTAATACATTATTGAGTGAAACATCTTCAAAGCTAGAT
    ATGCATGATGATTGTCGTGTTAAAGGATTTGGTAATACGACAACTAGTATACCTGGTATC
    TATGCGTGTGGAGATATTGTTTATCATGATGCAAAATCACATCTAATTGCAAGTGCATTT
    AGTGATGGTGCAAATGCAGCGAACCTTGCTAAAACATATATCCAACCAGATGCGAATGCA
    GAGGGTTATGTTTCAAGTCATCATGAAGTGTTTAAAGAAGCGAATAAGACTATTGTAAAT
    AAACATTTATACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1035

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002341
  • symbol: JSNZ_002341
  • description: NAD(P)/FAD-dependent oxidoreductase
  • length: 344
  • theoretical pI: 6.83429
  • theoretical MW: 38174.2
  • GRAVY: -0.268314

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 130.7)
    and 27 more
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 92.8)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 92.8)
    putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 86.8)
    Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 76.7)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 56.3)
    Metabolism Amino acid biosynthesis Glutamate family glutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 45.8)
    putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 41.8)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 40.8)
    Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 39.8)
    mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 36.9)
    Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 34.8)
    Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 34.4)
    thioredoxin and glutathione reductase (TIGR01438; EC 1.6.4.-; HMM-score: 32.4)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 32.3)
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 31)
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 28.1)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 27.8)
    mycofactocin system FadH/OYE family oxidoreductase 1 (TIGR03996; EC 1.-.-.-; HMM-score: 25.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis enzyme (TIGR00292; HMM-score: 24.4)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 24.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 23.4)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 19.6)
    lycopene cyclase family protein (TIGR01790; HMM-score: 18.3)
    flavin-dependent oxidoreductase, MSMEG_0569 family (TIGR04046; HMM-score: 17.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 14.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin protoporphyrinogen oxidase (TIGR00562; EC 1.3.3.4; HMM-score: 13.2)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 11)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 110.6)
    and 14 more
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 83.2)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 46.3)
    HI0933_like; HI0933-like protein Rossmann domain (PF03486; HMM-score: 43.8)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 30.8)
    Lys_Orn_oxgnase; L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase (PF13434; HMM-score: 28.7)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 26.6)
    Thi4; Thi4 family (PF01946; HMM-score: 24.2)
    FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 23)
    NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 21.6)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 21.4)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 20.1)
    Amino_oxidase; Flavin containing amine oxidoreductase (PF01593; HMM-score: 20.1)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 15.3)
    FMO-like; Flavin-binding monooxygenase-like (PF00743; HMM-score: 15.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9009
    • Cytoplasmic Membrane Score: 0.0835
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0155
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005715
    • TAT(Tat/SPI): 0.000504
    • LIPO(Sec/SPII): 0.001405
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYPEKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGEIYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTALDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHISEVVLEHVESGDTHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTSIPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEANKTIVNKHLY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Slade A Loutet, Marek J Kobylarz, Crystal H T Chau, Michael E P Murphy
IruO is a reductase for heme degradation by IsdI and IsdG proteins in Staphylococcus aureus.
J Biol Chem: 2013, 288(36);25749-59
[PubMed:23893407] [WorldCat.org] [DOI] (I p)

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