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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

organism: Staphylococcus aureus COL

locus tag: SACOL_RS02715 [old locus tag: SACOL0531 ]

pan locus tag^?: SAUPAN002225000

symbol: SACOL_RS02715

pan gene symbol^?: rsmI

synonym:
product: rRNA (cytidine-2'-O-)-methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SACOL_RS02715 [old locus tag: SACOL0531 ]
symbol: SACOL_RS02715
product: rRNA (cytidine-2'-O-)-methyltransferase
replicon: chromosome
strand: +
coordinates: 540920..541759
length: 840
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Location: NC_002951 (540920..541759) NCBI
BioCyc: SACOL_RS02715 BioCyc
MicrobesOnline: see SACOL0531

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

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ATGGCTGTATTATATTTAGTGGGCACACCAATTGGTAATTTAGCAGATATTACTTATAGA
GCAGTTGATGTATTGAAACGTGTTGATATGATTGCTTGTGAAGACACTAGAGTAACTAGT
AAACTGTGTAATCATTATGATATTCCAACTCCATTAAAGTCATATCACGAACATAACAAG
GATAAGCAGACTGCTTTTATCATTGAACAGTTAGAATTAGGTCTTGACGTTGCGCTCGTA
TCTGATGCTGGATTGCCCTTAATTAGTGATCCTGGATACGAATTAGTAGTGGCAGCCAGA
GAAGCTAATATTAAAGTAGAGACTGTGCCTGGACCTAATGCTGGGCTGACGGCTTTGATG
GCTAGTGGATTACCTTCATATGTATATACATTTTTAGGATTTTTGCCACGAAAAGAGAAA
GAAAAAAGTGCTGTATTAGAGCAACGTATGCATGAAAATAGCACATTAATTATATACGAA
TCACCGCATCGTGTGACAGATACATTAAAAACAATTGCAAAGATAGATGCAACACGACAA
GTATCACTAGGGCGTGAATTAACTAAGAAGTTCGAACAAATTGTAACTGATGATGTAACA
CAATTACAAGCATTGATTCAGCAAGGCGATGTACCATTGAAAGGCGAATTCGTTATCTTA
ATTGAAGGTGCTAAAGCGAACAATGAGATATCGTGGTTTGATGATTTATCTATCAATGAG
CATGTTGATCATTATATTCAAACTTCACAGATGAAACCAAAACAAGCTATTAAAAAAGTT
GCTGAAGAACGACAACTTAAAACGAATGAAGTATATAATATTTATCATCAAATAAGTTAA
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⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SACOL_RS02715 [old locus tag: SACOL0531 ]
symbol: SACOL_RS02715
description: rRNA (cytidine-2'-O-)-methyltransferase
length: 279
theoretical pI: 5.62169
theoretical MW: 31274.6
GRAVY: -0.208961

⊟Function[edit | edit source]

⊞reaction:
EC 2.1.1.198? ExPASy
16S rRNA (cytidine¹⁴⁰²-2'-O)-methyltransferase S-adenosyl-L-methionine + cytidine¹⁴⁰² in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine¹⁴⁰² in 16S rRNA
⊞TIGRFAM:
Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (cytidine(1402)-2'-O)-methyltransferase (TIGR00096; EC 2.1.1.198; HMM-score: 274.2)
and 6 more
Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-4 C11-methyltransferase (TIGR01465; EC 2.1.1.133; HMM-score: 25.2)
Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin uroporphyrinogen-III C-methyltransferase (TIGR01469; EC 2.1.1.107; HMM-score: 23.3)
Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-2 C(20)-methyltransferase (TIGR01467; EC 2.1.1.130; HMM-score: 20.5)
Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-3B C17-methyltransferase (TIGR01466; EC 2.1.1.131; HMM-score: 17.7)
Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other nitrogenase MoFe cofactor biosynthesis protein NifE (TIGR01283; HMM-score: 14.1)
Metabolism Central intermediary metabolism Nitrogen fixation nitrogenase MoFe cofactor biosynthesis protein NifE (TIGR01283; HMM-score: 14.1)
TheSEED: see SACOL0531
PFAM:
no clan defined TP_methylase; Tetrapyrrole (Corrin/Porphyrin) Methylases (PF00590; HMM-score: 132.4)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

⊞
PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helix: 1
⊞
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9996
- Cytoplasmic Membrane Score: 0.0002
- Cell wall & surface Score: 0.0001
- Extracellular Score: 0.0002
LocateP:
⊞
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.025341
- TAT(Tat/SPI): 0.000726
- LIPO(Sec/SPII): 0.004956
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 446202071 NCBI
RefSeq: WP_000279926 NCBI
UniProt: see SACOL0531

⊟Protein sequence[edit | edit source]

MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCNHYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREANIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYESPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFVILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYHQIS

⊟Experimental data[edit | edit source]

experimentally validated: see SACOL0531
protein localization: see SACOL0531
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

regulator:

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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