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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

organism: Staphylococcus aureus COL

locus tag: SACOL_RS02590 [old locus tag: SACOL0513 ]

pan locus tag^?: SAUPAN002185000

symbol: SACOL_RS02590

pan gene symbol^?: gltC

synonym:
product: LysR family transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SACOL_RS02590 [old locus tag: SACOL0513 ]
symbol: SACOL_RS02590
product: LysR family transcriptional regulator
replicon: chromosome
strand: -
coordinates: 512593..513477
length: 885
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Location: NC_002951 (512593..513477) NCBI
BioCyc: SACOL_RS02590 BioCyc
MicrobesOnline: see SACOL0513

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

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ATGGAAATTAAACAATTAAGATATTTTATCGAGGTCGCTAAAAGAGAACATATCTCCGAA
ACAGCACTTGAACTAAACATTGCTCAATCAGCGATAAGCCGTCAAATTACATTATTAGAA
CAAGAACTTAATGTGTCTTTATTTAAAAAGCAAGGACGCAATATTACGCTCACATCTGAA
GGGAAATTATTATTTAATGAAGCTCTGCGTATTATTGAACATTTAGATAGCACGATTGAA
CAATTTCAAAGCCATGGATTAACTAAAAATAAAAGTATCTACATTGGCTATGATGAGAGT
GACGTATCCCACATGTTACTACCACTTATCCAAACGTTTCATTTACAAAATGATACACAT
GTCATACCGAGCTTATTAGAACATGACACAATTATAAATAGCGTATTAAATGGGAATATA
GACATTGGTTTCACGGAACTTACACCAGAAATACGTAAACACAAACAATTAAATATGCTA
CCTTTATTCGAAGAGCACTATCATTTATATGCGCCTTCAGACGATCCAATTACGATGGCG
ACACACCCACCATTAATTCAGTTTGAGCATAGTCATATTTATTGCTTGGCACCATTTGCC
GAAACTGTAAAAAAACAATTGCGCAAGATTACTAAATCAGATGTATACACAATCAGTAGC
CAACCACTTGCGCAGTATCTCCTTCGTCAAAAAGAAGGTTACATCATTTCATCACAAAAT
ATACATTTACCAGAATCTAAATCATGGATAGATATAAAATTAGATCATACTGAATTAAAA
AGAACGATATGTGCTATTACAAAGGAGCCTTATACAAAGAGTGATATTGGCATATTATTA
ACACTTATTCAGCAATTAATGACTAAAACGTCTACATTCCATTAA
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⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SACOL_RS02590 [old locus tag: SACOL0513 ]
symbol: SACOL_RS02590
description: LysR family transcriptional regulator
length: 294
theoretical pI: 6.7243
theoretical MW: 33919.8
GRAVY: -0.258844

⊟Function[edit | edit source]

⊞TIGRFAM:
putative choline sulfate-utilization transcription factor (TIGR03418; HMM-score: 66.5)
Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 59.4)
Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 59.4)
and 5 more
Signal transduction Regulatory functions DNA interactions aminoethylphosphonate catabolism associated LysR family transcriptional regulator (TIGR03339; HMM-score: 50.6)
Signal transduction Regulatory functions DNA interactions D-serine deaminase transcriptional activator (TIGR02036; HMM-score: 40.5)
Cellular processes Cellular processes Toxin production and resistance transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.2)
Genetic information processing DNA metabolism DNA replication, recombination, and repair transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.2)
Signal transduction Regulatory functions DNA interactions transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.2)
TheSEED: see SACOL0513
⊞PFAM:
HTH (CL0123) HTH_1; Bacterial regulatory helix-turn-helix protein, lysR family (PF00126; HMM-score: 68.4)
and 3 more
PBP (CL0177) LysR_substrate; LysR substrate binding domain (PF03466; HMM-score: 34.8)
HTH (CL0123) HTH_34; Winged helix DNA-binding domain (PF13601; HMM-score: 17.4)
no clan defined Ribosomal_S13_N; Ribosomal S13/S15 N-terminal domain (PF08069; HMM-score: 17.2)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:
genes regulated by GltC, TF important in Glutamate metabolism: see SACOL0513

⊟Localization[edit | edit source]

⊞
PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
⊞
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.941
- Cytoplasmic Membrane Score: 0.0095
- Cell wall & surface Score: 0.0006
- Extracellular Score: 0.0489
LocateP:
⊞
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002278
- TAT(Tat/SPI): 0.000185
- LIPO(Sec/SPII): 0.000318
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 446326062 NCBI
RefSeq: WP_000403917 NCBI
UniProt: see SACOL0513

⊟Protein sequence[edit | edit source]

MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELNVSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDVSHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLNMLPLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTISSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDIGILLTLIQQLMTKTSTFH

⊟Experimental data[edit | edit source]

experimentally validated: data available for NCTC8325
protein localization: see SACOL0513
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

regulator: CodY, GltC see SACOL0513

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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