PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

Views

• Read
• Edit
• History

More

• Edit source

⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_1679 [new locus tag: NWMN_RS09420 ]
pan locus tag^?: SAUPAN004500000
symbol: NWMN_1679
pan gene symbol^?: smdA
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1679 [new locus tag: NWMN_RS09420 ]
• symbol: NWMN_1679
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1873375..1874283
• length: 909
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330996 NCBI
• RefSeq: YP_001332713 NCBI
• BioCyc:
• MicrobesOnline: 3707231 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  
  ATGGATTTATCTTCACCGATAGTCATTGGACTCATTATTGCAGTAGTCATTGCATTAATC
  TTTTTTGTCCTTTTTTTAGTTGCCTTAGGTAGTAAGAAAAAAGTTAAGCGACAAACAGAA
  GAAAAGTATGAACAACAAGAACAAAACATTAAAAAGTCTCATGAAGAAGCTTTAGAGAAA
  GAGCGTATTCAAAATAAAAAGACAATTACAAAACAACAAGAAGATTATAATCACATGGTT
  AGTACAAAAGATCGTGAGATTGACGCATTAAAATTGTTCTCGAAAAATCATAGTGAATAT
  GTAACGGACATGAGATTAATTGGTATTCGTGAAAGATTAGTTAAAGAAAAACGAATTCGA
  CCAGAAGATATGCACATTATGGCAAATATCTTTTTACCAAAGGATGGCTTTAATAATATT
  GAACGAATTAGTCATTTAGTATTAACAAGAACTGGTCTTTATATTATTGATTCTCAATTG
  TTAAAAGGTCATGTTTATAATGGTATTAGTGGCGGTCAATTTAAAGATTTACCACCAATG
  GAACAAGTATTTGATACATTGGATTTAGATAAATCTAGACCGCAAACTATTGTGATGGAT
  CAAAATGATGACAAACGTTCATTATCATTTGTAAATTATTCAGATCAAATTGAGGCGATT
  AAACAATTGGCTGAAGACTTACAAAAAGAATTAGGTGCCAAATATACACCAACTTCAATT
  TTATACTTTAATCTTAAAAATGAAGGCGATGTAACGATTTCAAATTATAATCAAAATAGT
  GCTGTCAAAGTGCTTGTCGGTGCTGAACAATTAGACGAGTTCTTTAACAAATTTGTATTC
  CATGGTCGTATTCAATACAACGTGGAAGACTTACAACAAATGATGGATAAAATTGAATCA
  TTCAATTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  909
  

⊟Protein

⊟General

• locus tag: NWMN_1679 [new locus tag: NWMN_RS09420 ]
• symbol: NWMN_1679
• description: hypothetical protein
• length: 302
• theoretical pI: 6.41787
• theoretical MW: 35052.9
• GRAVY: -0.457616

⊟Function

• ⊞TIGRFAM:
  cardiolipin synthase (TIGR04265; EC 2.7.8.-; HMM-score: 15.3)
  Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines nucleoside Transporter (TIGR00939; HMM-score: 12.3)
  and 5 more
  Metabolism Transport and binding proteins Unknown substrate efflux transporter, putative, hydrophobe/amphiphile efflux-3 (HAE3) family (TIGR00921; HMM-score: 12)
  Cellular processes Cellular processes Sporulation and germination transcription factor, RsfA family (TIGR02894; HMM-score: 11.5)
  Signal transduction Regulatory functions DNA interactions transcription factor, RsfA family (TIGR02894; HMM-score: 11.5)
  Unknown function General TIGR00374 family protein (TIGR00374; HMM-score: 11.3)
  Metabolism Energy metabolism Methanogenesis tetrahydromethanopterin S-methyltransferase, subunit B (TIGR04166; EC 2.1.1.86; HMM-score: 7)
• TheSEED  :
  • Hypothetical protein SAV1789
• ⊞PFAM:
  PDDEXK (CL0236) NERD; Nuclease-related domain (PF08378; HMM-score: 25.3)
  no clan defined DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 25.3)
  FlaX; FlaX (PF24151; HMM-score: 22.8)
  and 30 more
  Oxa1 (CL0376) EMC3_TMCO1; Integral membrane protein EMC3/TMCO1-like (PF01956; HMM-score: 17.6)
  MFS (CL0015) CLN3; CLN3 protein (PF02487; HMM-score: 16.2)
  no clan defined Tmemb_9; TMEM9 (PF05434; HMM-score: 15.6)
  FAM176; FAM176 family (PF14851; HMM-score: 15.6)
  IT (CL0182) ArsP_1; Predicted permease (PF03773; HMM-score: 15.2)
  no clan defined SecD-TM1; SecD export protein N-terminal TM region (PF13721; HMM-score: 15.2)
  TPR (CL0020) DUF6377; Domain of unknown function (DUF6377) (PF19904; HMM-score: 14.9)
  no clan defined DUF6779; Domain of unknown function (DUF6779) (PF20570; HMM-score: 14.5)
  Polo-box (CL0708) C5orf34_2nd; C5orf34-like, second domain (PF22833; HMM-score: 14.3)
  FixH-like (CL0488) FixH; FixH (PF05751; HMM-score: 13.9)
  no clan defined PFF1_TM; Vacuolar membrane protease, transmembrane domain (PF22251; HMM-score: 13.7)
  DMT (CL0184) CSG2; Ceramide synthase regulator (PF16965; HMM-score: 13.6)
  no clan defined DUF7394; Family of unknown function (DUF7394) (PF24126; HMM-score: 13.6)
  HTH (CL0123) WH_AprA; AprA winged helix domain (PF23589; HMM-score: 13.5)
  no clan defined EphA2_TM; Ephrin type-A receptor 2 transmembrane domain (PF14575; HMM-score: 13.3)
  DUF4446; Protein of unknown function (DUF4446) (PF14584; HMM-score: 13.3)
  DUF7807; Family of unknown function (DUF7807) (PF25093; HMM-score: 13.3)
  Cadherin_C_2; Cadherin cytoplasmic C-terminal (PF16492; HMM-score: 13.1)
  DUF7261; Family of unknown function (DUF7261) (PF23922; HMM-score: 13)
  Baculo_11_kDa; Baculovirus 11 kDa family (PF06143; HMM-score: 12.6)
  FraC; Filament integrity protein FraC (PF24301; HMM-score: 12.5)
  LapA_dom; Lipopolysaccharide assembly protein A domain (PF06305; HMM-score: 12.4)
  DUF3169; Protein of unknown function (DUF3169) (PF11368; HMM-score: 12.3)
  Transporter (CL0375) Connexin; Connexin (PF00029; HMM-score: 11.8)
  no clan defined DUF4006; Family of unknown function (DUF4006) (PF13179; HMM-score: 11.7)
  C_Lectin (CL0056) Ly49; Ly49-like protein, N-terminal region (PF08391; HMM-score: 10.8)
  no clan defined DUF4407; Domain of unknown function (DUF4407) (PF14362; HMM-score: 10.8)
  Piezo_TM1-24; Piezo TM1-24 (PF24871; HMM-score: 10.7)
  MtrB; Tetrahydromethanopterin S-methyltransferase subunit B (PF05440; HMM-score: 7.4)
  O-anti_assembly (CL0499) O-antigen_lig; O-antigen ligase like membrane protein (PF13425; HMM-score: 7.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helix: 1
• ⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0005
  • Cytoplasmic Membrane Score: 0.9635
  • Cell wall & surface Score: 0.0014
  • Extracellular Score: 0.0345
• ⊞LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: 4
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.017489
  • TAT(Tat/SPI): 0.000786
  • LIPO(Sec/SPII): 0.130279
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 151221891 NCBI
• RefSeq: YP_001332713 NCBI
• UniProt: A0A0H3K8W5 UniProt

⊟Protein sequence

• MDLSSPIVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYEQQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVTDMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQLLKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIEAIKQLAEDLQKELGAKYTPTSILYFNLKNEGDVTISNYNQNSAVKVLVGAEQLDEFFNKFVFHGRIQYNVEDLQQMMDKIESFN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications