PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA_RS11035 [old locus tag: SA1919 ]
pan locus tag^?: SAUPAN005408000
symbol: SA_RS11035
pan gene symbol^?: prmC
• synonym:
• product: protein-(glutamine-N5) methyltransferase, release factor-specific

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS11035 [old locus tag: SA1919 ]
• symbol: SA_RS11035
• product: protein-(glutamine-N5) methyltransferase, release factor-specific
• replicon: chromosome
• strand: -
• coordinates: 2167492..2168328
• length: 837
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2167492..2168328) NCBI
• BioCyc: SA_RS11035 BioCyc
• MicrobesOnline: see SA1919

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGTGAATTATAAAGAAAAGTTAGATGAAGCAATTCATTTAACACAACAAAAAGGGTTT
  GAACAAACACGAGCTGAATGGTTAATGTTAGATGTATTTCAATGGACGCGTACGGACTTT
  GTAGTTCACATGCATGATGATATGCCGAAAGCGATGATTATGAAGTTCGACTTAGCATTA
  CAACGTATGTTATTAGGAGAGCCTATACAGTATATAGTTGGCTTTGCCTCATTTTATGGT
  AGAACGTTTGATGTAAACTCAAATTGTTTGATACCAAGACCTGAAACTGAAGAAGTAATG
  TTGCATTTCTTACAACAGTTAGAAGATGATGCAACAATCGTAGATATCGGAACGGGTAGT
  GGTGTACTTGCAATTACTTTGAAATGTGAAAAGCCGGATTTAAATGTTATTGCTACTGAT
  ATTTCACTTGAAGCAATGAATATGGCTCGTAATAATGCTGAGAAGCATCAATCACAAATA
  CAATTTTTAACAGGGGATGCATTAAAGCCCTTAATTAAAGAAGGTATCAAGTTGAACGGC
  TTGATATCTAATCCACCATATATAGATGAAAAAGATATGGTTACGATGTCTCCAACGGTT
  ACGAGATTCGAACCACATCAGGCATTGTTTGCAGATAACCATGGATATGCTATTTATGAA
  TCAATCATTGGAGATTTACCTCACGTTATGGAAAAAGGCAGCCCAGTTGTTTTTGAAATT
  GGTTACAATCAAGGTGAGGCACTTAAATCAATAATTTTAAATAAATTTCCTGACAAAAAA
  ATCGACATTATTAAAGATATAAATGGCCACGATCGAATCGTCTCATTTAAATGGTAA
  60
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  837
  

⊟Protein

⊟General

• locus tag: SA_RS11035 [old locus tag: SA1919 ]
• symbol: SA_RS11035
• description: protein-(glutamine-N5) methyltransferase, release factor-specific
• length: 278
• theoretical pI: 4.93525
• theoretical MW: 31672.3
• GRAVY: -0.182014

⊟Function

• ⊞reaction:
  EC 2.1.1.297?  ExPASy
  Peptide chain release factor N⁵-glutamine methyltransferase S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N⁵-methyl-L-glutamine
• ⊞TIGRFAM:
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 258)
  and 20 more
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 206.3)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 113.5)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification putative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 71.3)
  Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 46.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 32.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 31.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 28.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 27.4)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 26.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 23.4)
  Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 22.5)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 20.6)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 19.6)
  Genetic information processing Protein fate Protein modification and repair protein-L-isoaspartate O-methyltransferase (TIGR00080; EC 2.1.1.77; HMM-score: 15.1)
  Genetic information processing Transcription RNA processing 3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 14)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 14)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG (TIGR00138; EC 2.1.1.170; HMM-score: 13.9)
  2-ketoarginine methyltransferase (TIGR04543; EC 2.1.1.243; HMM-score: 13.2)
  Unknown function Enzymes of unknown specificity tRNA (cmo5U34)-methyltransferase (TIGR00740; EC 2.1.1.-; HMM-score: 12.8)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides putative sugar O-methyltransferase (TIGR04371; EC 2.1.1.-; HMM-score: 11.4)
• TheSEED: see SA1919
• ⊞PFAM:
  NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 56.4)
  no clan defined PrmC_N; PrmC N-terminal domain (PF17827; HMM-score: 55.3)
  and 20 more
  NADP_Rossmann (CL0063) Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 35.2)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 32.3)
  PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 31.4)
  Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 28.6)
  PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 25.9)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 24.6)
  Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 24)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 23.4)
  Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 20.7)
  Methyltransf_2; O-methyltransferase domain (PF00891; HMM-score: 19.6)
  Methyltransf_16; Lysine methyltransferase (PF10294; HMM-score: 19.1)
  NpmA; 16S rRNA (adenine(1408)-N(1))-methyltransferase (PF24675; HMM-score: 17.9)
  GidB; rRNA small subunit methyltransferase G (PF02527; HMM-score: 17.7)
  Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 16.9)
  Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 16.6)
  TrmK; tRNA (adenine(22)-N(1))-methyltransferase (PF04816; HMM-score: 16.5)
  Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 15.3)
  UPF0020; RMKL-like, methyltransferase domain (PF01170; HMM-score: 14.5)
  RMNT_CmcI; Rhamnosyl O-methyltransferase/CmcI (PF04989; HMM-score: 13.6)
  CMAS; Mycolic acid cyclopropane synthetase (PF02353; HMM-score: 12.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9971
  • Cytoplasmic Membrane Score: 0.0008
  • Cell wall & surface Score: 0.0004
  • Extracellular Score: 0.0017
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001389
  • TAT(Tat/SPI): 0.000134
  • LIPO(Sec/SPII): 0.000249
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446170877 NCBI
• RefSeq: WP_000248732 NCBI
• UniProt: see SA1919

⊟Protein sequence

• MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHMHDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLHFLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQIQFLTGDALKPLIKEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIGDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSFKW

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications