⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_RS07440 [old locus tag: NWMN_1322 ]
pan locus tag^?: SAUPAN003827000
symbol: NWMN_RS07440
pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS07440 [old locus tag: NWMN_1322 ]
• symbol: NWMN_RS07440
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1453280..1454071
• length: 792
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1453280..1454071) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1322

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  421
  481
  541
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  721
  781
  
  ATGGCACTAAAACATTATAAGAATTCAGATTCAACAGTTTTCAATGATGCGAAGGCATTA
  TTTGATTTAAATAAAAATATTTTACTTAAAGGTCCAACAGGTTCAGGGAAAACAAAGTTG
  GCAGAAACATTAAGTGAAGTTGTTGATACACCCATGCATCAAGTCAATTGTTCTGTTGAT
  TTAGATACAGAAAGCTTATTAGGCTTTAAAACAATTAAAACAAATGCGGAAGGTCAACAA
  GAAATTGTCTTTGTAGATGGTCCAGTTATTAAAGCTATGAAAGAGGGGCATATTTTATAT
  ATTGATGAAATAAATATGGCTAAACCTGAAACATTGCCTGTATTAAATGGGGTCTTAGAT
  TATCGTCGTCAAATTACGAATCCATACACTGGTGAAGTAATCAAAGCTGTACCAGGATTT
  AACGTTATAGCAGCGATAAATGAAGGTTATGTTGGTACTTTGCCAATGAATGAAGCACTA
  AAAAATCGCTTTGTTGTTATTCACGTTGATTATATTGATGGGGACATTTTAAAAAATGTG
  ATTAAAGAGCAAAGTTTATTACAAGATGATAAACAAATCGAACAAATTATTAAGTTTAAT
  GAAGATTTACGTACTATGTCTAAGCAGGGACAAATTTCTGAAGAAGCCGCTAGTATCCGT
  GCATTATTAGACTTGTGTGATTTAATCACTGTAATGCCAGTTGAACGTGCAATTAAACGT
  ACAATTATTGATAAATTGGAAGATGAACGTGAACAACAAGCAATATATAATGCTGTAGAA
  CTAAACTTTTAA
  60
  120
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  240
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  360
  420
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  540
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  792
  

⊟Protein

⊟General

• locus tag: NWMN_RS07440 [old locus tag: NWMN_1322 ]
• symbol: NWMN_RS07440
• description: hypothetical protein
• length: 263
• theoretical pI: 4.68695
• theoretical MW: 29448.6
• GRAVY: -0.186312

⊟Function

• ⊞⊞TIGRFAM:
  Cellular processes Cellular processes Other gas vesicle protein GvpN (TIGR02640; HMM-score: 75.1)
  and 11 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobaltochelatase, CobS subunit (TIGR01650; EC 6.6.1.2; HMM-score: 34.8)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent Clp protease, ATP-binding subunit ClpX (TIGR00382; HMM-score: 30.2)
  Genetic information processing Protein fate Protein folding and stabilization ATP-dependent Clp protease, ATP-binding subunit ClpX (TIGR00382; HMM-score: 30.2)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 27.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium chelatase ATPase subunit D (TIGR02031; EC 6.6.1.1; HMM-score: 20.5)
  Genetic information processing Protein fate Protein folding and stabilization ATP-dependent protease HslVU, ATPase subunit (TIGR00390; HMM-score: 18.7)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 14.3)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair orc1/cdc6 family replication initiation protein (TIGR02928; HMM-score: 14.1)
  Cellular processes Cellular processes Conjugation P-type conjugative transfer ATPase TrbB (TIGR02782; HMM-score: 13.6)
  thiol reductant ABC exporter, CydC subunit (TIGR02868; HMM-score: 13.3)
  Unknown function General Mg chelatase-like protein (TIGR00368; HMM-score: 12.2)
• TheSEED: see NWMN_1322
• ⊞⊞PFAM:
  P-loop_NTPase (CL0023) AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 99.7)
  and 30 more
  AAA_3; ATPase family associated with various cellular activities (AAA) (PF07726; HMM-score: 45.2)
  AAA_7; P-loop containing dynein motor region (PF12775; HMM-score: 27.5)
  bpMoxR; MoxR domain in the MoxR-vWA-beta-propeller ternary systems (PF20030; HMM-score: 26)
  Sigma54_activat; Sigma-54 interaction domain (PF00158; HMM-score: 23.4)
  AAA_lid (CL0671) CbbQ_C; CbbQ/NirQ/NorQ C-terminal (PF08406; HMM-score: 23.3)
  P-loop_NTPase (CL0023) AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 22.7)
  AAA_2; AAA domain (Cdc48 subfamily) (PF07724; HMM-score: 21.1)
  AAA_14; AAA domain (PF13173; HMM-score: 20.9)
  RuvB_N; Holliday junction DNA helicase RuvB P-loop domain (PF05496; HMM-score: 20.6)
  nSTAND3; Novel STAND NTPase 3 (PF20720; HMM-score: 20.6)
  Mg_chelatase; Magnesium chelatase, subunit ChlI (PF01078; HMM-score: 19.3)
  AAA_6; Hydrolytic ATP binding site of dynein motor region (PF12774; HMM-score: 17.3)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 17.3)
  T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 16.5)
  IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 16.3)
  AAA_18; AAA domain (PF13238; HMM-score: 16.3)
  AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 15.6)
  AAA_22; AAA domain (PF13401; HMM-score: 15.3)
  ABC_tran; ABC transporter (PF00005; HMM-score: 14.9)
  CPT; Chloramphenicol phosphotransferase-like protein (PF07931; HMM-score: 14.4)
  ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 14.2)
  AAA_PrkA; PrkA AAA domain (PF08298; HMM-score: 13.9)
  ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 13.5)
  DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 13.2)
  TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 13.2)
  AAA_17; AAA domain (PF13207; HMM-score: 13.2)
  Zeta_toxin; Zeta toxin (PF06414; HMM-score: 13.1)
  AAA_30; AAA domain (PF13604; HMM-score: 12.5)
  AAA_lid (CL0671) AAA_lid_7; Midasin AAA lid domain (PF17867; HMM-score: 12.5)
  P-loop_NTPase (CL0023) AAA_23; AAA domain (PF13476; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9898
  • Cytoplasmic Membrane Score: 0.0008
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0094
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006207
  • TAT(Tat/SPI): 0.000694
  • LIPO(Sec/SPII): 0.000454
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447108165 NCBI
• RefSeq: WP_001185421 NCBI
• UniProt: see NWMN_1322

⊟Protein sequence

• MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETLSEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYIDEINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEALKNRFVVIHVDYIDGDILKNVIKEQSLLQDDKQIEQIIKFNEDLRTMSKQGQISEEAASIRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications