PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS12660 [old locus tag: SACOL2413 ]
pan locus tag^?: SAUPAN005949000
symbol: SACOL_RS12660
pan gene symbol^?: mdeA
• synonym:
• product: drug:proton antiporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS12660 [old locus tag: SACOL2413 ]
• symbol: SACOL_RS12660
• product: drug:proton antiporter
• replicon: chromosome
• strand: -
• coordinates: 2472216..2473619
• length: 1404
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2472216..2473619) NCBI
• BioCyc: SACOL_RS12660 BioCyc
• MicrobesOnline: see SACOL2413

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  121
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  421
  481
  541
  601
  661
  721
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  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  
  ATGCTGATGGGTGGATTTTTTGGATTATTAAATGAAACACTATTAGTGACGGCTTTACCA
  AGTATTATGAAAGATTTTGAAATTTCATATACACAAGTTCAATGGCTGACAACAGCTTTT
  TTATTGACTAATGGGATTGTTATTCCTTTGTCCGCGCTTGTTATACAACGTTATACAACA
  AGACAAGTGTTTTTAGTGGGTATTTCTATCTTTTTCTTAGGTACATTACTCGGCGGCTTG
  AGTCCGCACTTTGCAACATTATTAGTTGCTAGAATTATTCAGGCGTTAGGCGCAGGTATT
  ATGATGCCATTGATGATGACAACGATTTTGGATGTTTTCCAACCACATGAACGCGGTAAA
  TATATGGGGATATTTGGTTTGGTAATTGGTTTAGCACCAGCTATTGGACCTACTCTTTCA
  GGTTACCTTGTTGAATATTTTAACTGGAGATCGCTTTTCCATGTTGTCGCTCCAATTGCA
  GCTGTGACATTTTTAATTGGATTTAAAACGATAAAAAATGTTGGAACTACAATTAAAGTA
  CCTATTGATTTTATTTCTGTCATTTTTTCTGTACTAGGTTTCGGCGGGTTATTGTATGGA
  ACGAGTTCAATTTCAGAAAAAGGTTTTGATAATCCTATCGTATTAGTATCTATGATTGGA
  GGCGTTGTTTTAGTCGCATTATTTGTATTACGTCAATATCGGCTATCAACACCATTATTA
  AATTTTGCTGTATTTAAAAATAAACAATTTACAGTTGGTATCATTATTATGGGTGTCACA
  ATGGTATCGATGATTGGTTCGGAAACGATTTTACCTATCTTTGTGCAAAATTTATTGCAT
  CGTTCAGCTTTAGATTCTGGATTAACTTTATTACCAGGAGCAATTGTTATGGCATTTATG
  TCGATGACTTCGGGTGCTTTATATGAAAAGTTTGGTCCTAGAAATCTTGCTTTAGTAGGT
  ATGGCGATTGTTGTTATTACTACGGCTTATTTTGTTGTAATGGATGAACAAACATCAACA
  ATCATGTTGGCAACAGTTTATGCGATTCGAATGGTTGGTATCGCGTTAGGATTAATTCCA
  GTAATGACCCATACGATGAATCAATTAAAGCCAGAAATGAATGCACATGGTTCATCTATG
  ACAAACACAGTACAACAAATTGCTGGTTCTATCGGAACAGCTGCATTAATTACTATTTTA
  TCCCATGCAAGCAAAAACTTTTCTCCAACTATGTCAGACTATAATGGCATGAACAAAATT
  GATATGATGAATCAAATTAAAGTTGATACGATGTTGCATGGTTATCACGCTGGATTTTTA
  TTTGCATTATTGATTACAGTTGTTAGTTTCTTTTGTTCATTTATGTTACAAGGTAAGAAA
  AAAGAAGTTGATTCTCGTCAATAA
  60
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  1260
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  1404
  

⊟Protein

⊟General

• locus tag: SACOL_RS12660 [old locus tag: SACOL2413 ]
• symbol: SACOL_RS12660
• description: drug:proton antiporter
• length: 467
• theoretical pI: 9.76001
• theoretical MW: 50784.4
• GRAVY: 0.775803

⊟Function

• ⊞TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 336.4)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 336.4)
  and 16 more
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 94.3)
  multidrug resistance protein (TIGR00880; HMM-score: 81.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 49.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 48.5)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 47.2)
  Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 34.1)
  Metabolism Transport and binding proteins Anions Na(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 26.5)
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 26.5)
  oxalate/formate antiporter (TIGR04259; HMM-score: 26.5)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids monocarboxylate transporter (TIGR00892; HMM-score: 23.3)
  Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 22.8)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 19.4)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 17.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids polyol permease (TIGR00897; HMM-score: 14.1)
  synaptic vesicle protein SV2 (TIGR01299; HMM-score: 7)
  sodium-independent organic anion transporter (TIGR00805; HMM-score: 6.5)
• TheSEED: see SACOL2413
• ⊞PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 194.8)
  and 2 more
  MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 35.4)
  OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 18)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• ⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 1
  • Cell wall & surface Score: 0
  • Extracellular Score: 0
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006867
  • TAT(Tat/SPI): 0.000203
  • LIPO(Sec/SPII): 0.010616
• predicted transmembrane helices (TMHMM): 13

⊟Accession numbers

• GI: see SACOL2413
• RefSeq: WP_072498116 NCBI
• UniProt: see SACOL2413

⊟Protein sequence

• MLMGGFFGLLNETLLVTALPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVARIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFNWRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMAIVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: see SACOL2413
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: Zur* see SACOL2413

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications