PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_002386
• pan locus tag^?: SAUPAN005949000
• symbol: mdeA
• pan gene symbol^?: mdeA
• synonym:
• product: multidrug efflux MFS transporter MdeA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_002386
• symbol: mdeA
• product: multidrug efflux MFS transporter MdeA
• replicon: chromosome
• strand: -
• coordinates: 2382948..2384387
• length: 1440
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  
  ATGTCGAAGAAACAAAAATTAACGATGATTATTACTATGCTGATGGGTGGATTTTTTGGA
  TTATTAAATGAAACACTATTAGTGACGGCTTTACCAAGTATTATGAAAGATTTTGAAATT
  TCATATACACAAGTTCAATGGCTGACAACAGCTTTTTTATTGACTAATGGGATTGTTATT
  CCTTTGTCCGCGCTTGTTATACAACGTTATACAACAAGACAAGTGTTTTTAGTGGGTATT
  TCTATCTTTTTCTTAGGTACATTACTCGGCGGTTTGAGTCCGCACTTTGCAACATTATTA
  GTTGCTAGAATTATTCAGGCGTTAGGTGCAGGTATTATGATGCCATTGATGATGACAACG
  ATTTTGGATGTTTTCCAACCGCATGAACGCGGTAAATATATGGGGATATTTGGTTTGGTA
  ATTGGTTTAGCACCAGCTATTGGACCTACTCTTTCAGGTTACCTTGTTGAATATTTTAAC
  TGGAGATCGCTTTTCCATGTTGTCGCTCCAATTGCAGCTGTTACATTTTTAATTGGATTT
  AAAACGATAAAAAATGTTGGAACTACAATTAAAGTACCTATTGATTTTATTTCTGTCATT
  TTTTCTGTACTAGGTTTCGGCGGGTTATTGTATGGAACGAGTTCAATTTCAGAAAAAGGT
  TTTGATAATCCTATCGTATTAGTATCTATGATTGGAGGCGTTGTTTTAGTCGCATTATTT
  GTATTACGTCAATATCGGCTATCAACACCATTATTAAATTTTGCTGTATTTAAAAATAAA
  CAATTTACAGTTGGTATCATTATTATGGGTGTCACAATGGTATCGATGATTGGTTCGGAA
  ACGATTTTACCTATCTTTGTGCAAAATTTATTGCATCGTTCAGCTTTAGATTCTGGGTTA
  ACTTTATTACCAGGAGCAATTGTTATGGCATTTATGTCGATGACTTCGGGTGCTTTATAT
  GAAAAGTTTGGTCCTAGAAAGCTTGCTTTAGTCGGTATGGCGATTGTTGTTATTACTACG
  GCTTATTTTGTTGTAATGGATGAACAAACATCAACAATCATGTTGGCAACAGTTTATGCG
  ATTCGAATGGTTGGTATCGCGTTAGGATTAATTCCAGTAATGACCCATACGATGAATCAA
  TTAAAGCCAGAAATGAATGCACACGGTTCATCTATGACAAACACAGTACAACAAATTGCT
  GGTTCTATCGGAACAGCTGCATTAATTACTATTTTATCTCATGCAAGCAAAAACTTTTCT
  CCAACTATGTCAGACTATAATGGCATGAACAAAATTGATATGATGAATCAAATTAAAGTT
  GATACGATGTTGCATGGTTATCACGCTGGATTTTTATTTGCATTATTGATTACAGTTGTT
  AGTTTCTTTTGTTCATTTATGTTACAAGGGAAGAAAAAAGAAGTTGATTCTCGTCAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  

⊟Protein

⊟General

• locus tag: JSNZ_002386
• symbol: MdeA
• description: multidrug efflux MFS transporter MdeA
• length: 479
• theoretical pI: 10.0767
• theoretical MW: 52202.3
• GRAVY: 0.753862

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 339.5)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 339.5)
  and 16 more
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 94.1)
  multidrug resistance protein (TIGR00880; HMM-score: 81.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 50.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 48.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 48)
  Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 32.3)
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 28.7)
  oxalate/formate antiporter (TIGR04259; HMM-score: 26.5)
  Metabolism Transport and binding proteins Anions Na(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 25)
  Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 23.8)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids monocarboxylate transporter (TIGR00892; HMM-score: 22.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 20.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids polyol permease (TIGR00897; HMM-score: 15.5)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 15.4)
  sodium-independent organic anion transporter (TIGR00805; HMM-score: 5.8)
  synaptic vesicle protein SV2 (TIGR01299; HMM-score: 5.3)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 195.6)
  and 3 more
  MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 33.4)
  OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 16.7)
  GPCR_A (CL0192) DUF6962; Family of unknown function (DUF6962) (PF22285; HMM-score: 7.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 1
  • Cell wall & surface Score: 0
  • Extracellular Score: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.25134
  • TAT(Tat/SPI): 0.000869
  • LIPO(Sec/SPII): 0.010949
• predicted transmembrane helices (TMHMM): 14

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVARIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFNWRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRKLALVGMAIVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: Zur* (repression) regulon

  Zur*

  (TF)

  important in Zinc homeostasis; transcription unit predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications