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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002386
  • pan locus tag?: SAUPAN005949000
  • symbol: mdeA
  • pan gene symbol?: mdeA
  • synonym:
  • product: multidrug efflux MFS transporter MdeA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002386
  • symbol: mdeA
  • product: multidrug efflux MFS transporter MdeA
  • replicon: chromosome
  • strand: -
  • coordinates: 2382948..2384387
  • length: 1440
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCGAAGAAACAAAAATTAACGATGATTATTACTATGCTGATGGGTGGATTTTTTGGA
    TTATTAAATGAAACACTATTAGTGACGGCTTTACCAAGTATTATGAAAGATTTTGAAATT
    TCATATACACAAGTTCAATGGCTGACAACAGCTTTTTTATTGACTAATGGGATTGTTATT
    CCTTTGTCCGCGCTTGTTATACAACGTTATACAACAAGACAAGTGTTTTTAGTGGGTATT
    TCTATCTTTTTCTTAGGTACATTACTCGGCGGTTTGAGTCCGCACTTTGCAACATTATTA
    GTTGCTAGAATTATTCAGGCGTTAGGTGCAGGTATTATGATGCCATTGATGATGACAACG
    ATTTTGGATGTTTTCCAACCGCATGAACGCGGTAAATATATGGGGATATTTGGTTTGGTA
    ATTGGTTTAGCACCAGCTATTGGACCTACTCTTTCAGGTTACCTTGTTGAATATTTTAAC
    TGGAGATCGCTTTTCCATGTTGTCGCTCCAATTGCAGCTGTTACATTTTTAATTGGATTT
    AAAACGATAAAAAATGTTGGAACTACAATTAAAGTACCTATTGATTTTATTTCTGTCATT
    TTTTCTGTACTAGGTTTCGGCGGGTTATTGTATGGAACGAGTTCAATTTCAGAAAAAGGT
    TTTGATAATCCTATCGTATTAGTATCTATGATTGGAGGCGTTGTTTTAGTCGCATTATTT
    GTATTACGTCAATATCGGCTATCAACACCATTATTAAATTTTGCTGTATTTAAAAATAAA
    CAATTTACAGTTGGTATCATTATTATGGGTGTCACAATGGTATCGATGATTGGTTCGGAA
    ACGATTTTACCTATCTTTGTGCAAAATTTATTGCATCGTTCAGCTTTAGATTCTGGGTTA
    ACTTTATTACCAGGAGCAATTGTTATGGCATTTATGTCGATGACTTCGGGTGCTTTATAT
    GAAAAGTTTGGTCCTAGAAAGCTTGCTTTAGTCGGTATGGCGATTGTTGTTATTACTACG
    GCTTATTTTGTTGTAATGGATGAACAAACATCAACAATCATGTTGGCAACAGTTTATGCG
    ATTCGAATGGTTGGTATCGCGTTAGGATTAATTCCAGTAATGACCCATACGATGAATCAA
    TTAAAGCCAGAAATGAATGCACACGGTTCATCTATGACAAACACAGTACAACAAATTGCT
    GGTTCTATCGGAACAGCTGCATTAATTACTATTTTATCTCATGCAAGCAAAAACTTTTCT
    CCAACTATGTCAGACTATAATGGCATGAACAAAATTGATATGATGAATCAAATTAAAGTT
    GATACGATGTTGCATGGTTATCACGCTGGATTTTTATTTGCATTATTGATTACAGTTGTT
    AGTTTCTTTTGTTCATTTATGTTACAAGGGAAGAAAAAAGAAGTTGATTCTCGTCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002386
  • symbol: MdeA
  • description: multidrug efflux MFS transporter MdeA
  • length: 479
  • theoretical pI: 10.0767
  • theoretical MW: 52202.3
  • GRAVY: 0.753862

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 339.5)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 339.5)
    and 16 more
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 94.1)
    multidrug resistance protein (TIGR00880; HMM-score: 81.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 50.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 48.3)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 48)
    Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 32.3)
    Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 28.7)
    oxalate/formate antiporter (TIGR04259; HMM-score: 26.5)
    Metabolism Transport and binding proteins Anions Na(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 25)
    Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 23.8)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids monocarboxylate transporter (TIGR00892; HMM-score: 22.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 20.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids polyol permease (TIGR00897; HMM-score: 15.5)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 15.4)
    sodium-independent organic anion transporter (TIGR00805; HMM-score: 5.8)
    synaptic vesicle protein SV2 (TIGR01299; HMM-score: 5.3)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 195.6)
    and 3 more
    MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 33.4)
    OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 16.7)
    GPCR_A (CL0192) DUF6962; Family of unknown function (DUF6962) (PF22285; HMM-score: 7.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 1
    • Cell wall & surface Score: 0
    • Extracellular Score: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.25134
    • TAT(Tat/SPI): 0.000869
    • LIPO(Sec/SPII): 0.010949
  • predicted transmembrane helices (TMHMM): 14

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVARIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFNWRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRKLALVGMAIVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Zur* (repression) regulon
    Zur*(TF)important in Zinc homeostasis;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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