⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA0471 [new locus tag: SA_RS02710 ]
pan locus tag^?: SAUPAN002255000
symbol: cysK
pan gene symbol^?: cysK
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0471 [new locus tag: SA_RS02710 ]
• symbol: cysK
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 544265..545197
• length: 933
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123262 NCBI
• RefSeq: NP_373723 NCBI
• BioCyc: see SA_RS02710
• MicrobesOnline: 102749 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
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  481
  541
  601
  661
  721
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  841
  901
  
  ATGGCACAAAAACCAGTAGATAATATTACTCAAATTATTGGCGGTACACCGGTAGTCAAA
  TTGAGAAATGTAGTAGATGACAATGCAGCAGATGTTTATGTAAAATTGGAATATCAAAAT
  CCAGGTGGTTCTGTAAAGGATAGAATTGCTTTAGCAATGATTGAAAAAGCAGAGCGAGAA
  GGCAAAATTAAACCTGGCGACACAATTGTAGAACCAACAAGTGGTAATACAGGTATCGGT
  TTAGCATTTGTATGTGCTGCTAAAGGATATAAAGCAGTATTTACTATGCCCGAAACAATG
  AGCCAAGAGCGTCGTAATTTATTAAAAGCATACGGTGCGGAATTAGTTTTAACGCCTGGA
  TCAGAAGCGATGAAAGGTGCAATTAAAAAAGCTAAAGAATTAAAAGAAGAACATGGTTAC
  TTCGAGCCACAACAATTTGAAAACCCTGCGAACCCTGAAGTTCATGAGTTAACTACAGGT
  CCTGAGTTATTACAACAATTTGAAGGGAAAACTATCGATGCGTTCCTAGCTGGTGTTGGT
  ACTGGTGGTACGTTATCTGGTGTAGGTAAAGTTCTGAAAAAAGAATATCCTAACATCGAA
  ATTGTTGCTATAGAGCCTGAGGCTTCTCCAGTATTGAGCGGTGGTGAGCCAGGTCCACAT
  AAATTACAAGGTTTAGGTGCTGGATTTATTCCAGGCACTTTGAATACAGAAATCTATGAC
  AGTATTATTAAAGTAGGAAATGATACAGCGATGGAAATGTCTCGTCGAGTTGCTAAAGAG
  GAAGGTATTTTAGCAGGTATTTCATCAGGTGCTGCGATTTATGCTGCCATTCAAAAAGCA
  AAAGAATTAGGAAAAGGTAAAACAGTAGTAACAGTATTGCCGAGTAATGGTGAACGCTAC
  TTATCAACACCTTTATATTCATTCGATGACTAA
  60
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  933
  

⊟Protein

⊟General

• locus tag: SA0471 [new locus tag: SA_RS02710 ]
• symbol: CysK
• description: hypothetical protein
• length: 310
• theoretical pI: 5.12885
• theoretical MW: 32975.4
• GRAVY: -0.237419

⊟Function

• ⊞⊞reaction:
  EC 2.5.1.47?  ExPASy
  Cysteine synthase O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
• ⊞⊞TIGRFAM:
  Metabolism Amino acid biosynthesis Serine family cysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 463.6)
  Metabolism Amino acid biosynthesis Serine family cysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 440.6)
  and 12 more
  Metabolism Amino acid biosynthesis Serine family cystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 326.6)
  Metabolism Amino acid biosynthesis Serine family cysteine synthase B (TIGR01138; EC 2.5.1.47; HMM-score: 293)
  Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnA (TIGR03945; HMM-score: 245.2)
  Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase, biosynthetic (TIGR01124; EC 4.3.1.19; HMM-score: 73.8)
  Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase (TIGR01127; EC 4.3.1.19; HMM-score: 73.7)
  Metabolism Amino acid biosynthesis Pyruvate family threonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 72.7)
  ectoine utilization protein EutB (TIGR02991; HMM-score: 48)
  Metabolism Amino acid biosynthesis Aspartate family threonine synthase (TIGR00260; EC 4.2.3.1; HMM-score: 41.3)
  Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, beta subunit (TIGR00263; EC 4.2.1.20; HMM-score: 33.4)
  Unknown function Enzymes of unknown specificity pyridoxal-phosphate dependent TrpB-like enzyme (TIGR01415; HMM-score: 33.4)
  diaminopropionate ammonia-lyase (TIGR03528; EC 4.3.1.15; HMM-score: 23.2)
  Metabolism Energy metabolism Other diaminopropionate ammonia-lyase family (TIGR01747; EC 4.3.1.15; HMM-score: 23.1)
• ⊞⊞TheSEED  :
  • Cysteine synthase (EC 2.5.1.47)
  Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Cysteine Biosynthesis  Cysteine synthase (EC 2.5.1.47)
  and 1 more
  Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  Cysteine synthase (EC 2.5.1.47)
• ⊞⊞PFAM:
  NADP_Rossmann (CL0063) PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 270.5)
  and 2 more
  ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 19.6)
  Thiolase (CL0046) ACP_syn_III_C; 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal (PF08541; HMM-score: 11)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: pyridoxal 5'-phosphate
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.992
  • Cytoplasmic Membrane Score: 0.0016
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0062
• ⊞⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003491
  • TAT(Tat/SPI): 0.000228
  • LIPO(Sec/SPII): 0.000529
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926190 NCBI
• RefSeq: NP_373723 NCBI
• UniProt: P63871 UniProt

⊟Protein sequence

• MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGSVKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTGPELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPGPHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAAIQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• ⊟⊟interaction partners:

  SA1984	(asp23)	alkaline shock protein 23   (data from MRSA252)
  SA0731	(eno)	phosphopyruvate hydratase   (data from MRSA252)
  SA0505	(fus)	elongation factor G   (data from MRSA252)
  SA0727	(gap)	glyceraldehyde-3-phosphate dehydrogenase   (data from MRSA252)
  SA2400	(mqo2)	malate:quinone oxidoreductase   (data from MRSA252)
  SA0943-1	(pdhA)	pyruvate dehydrogenase E1 component subunit alpha   (data from MRSA252)
  SA0944	(pdhB)	pyruvate dehydrogenase E1 component subunit beta   (data from MRSA252)
  SA0946	(pdhD)	dihydrolipoamide dehydrogenase   (data from MRSA252)
  SA1938	(pdp)	pyrimidine-nucleoside phosphorylase   (data from MRSA252)
  SA1520	(pykA)	pyruvate kinase   (data from MRSA252)
  SA0496	(rplA)	50S ribosomal protein L1   (data from MRSA252)
  SA0497	(rplJ)	50S ribosomal protein L10   (data from MRSA252)
  SA0506	(tuf)	elongation factor Tu   (data from MRSA252)
  SA1453		hypothetical protein   (data from MRSA252)

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• ⊞⊞regulator: CymR* (repression) regulon

  CymR*

  (TF)

  important in Cysteine metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications